The spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body

Journal of Cell Science

Volumn 109, Issue 9, 1996, Pages 2229-2237

  Spang, Anne ^a   Grein, Katrin ^a   Schiebel, Elmar ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

Calmodulin; Spc110p; Spindle pole body; Yeast

Indexed keywords

CALMODULIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CELL ORGANELLE; CELL ULTRASTRUCTURE; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; YEAST;

AMINO ACID SEQUENCE; BINDING SITES; CALCIUM; CALMODULIN; CENTROSOME; DNA PRIMERS; FUNGAL PROTEINS; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PEPTIDES; PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0029837818     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Adams, A. E. M. and Pringle, J. (1989). Relationship of actin and tubulin to budgrowth in wild-type and morphogenetic-mutant Saccharomyces cerevisiae. J. Cell Biol. 108, 934-945.
2. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. (1994). Current Protocols in Molecular Biology. John Wiley and Sons, New York.
3. Brockerhoff, S. E., and Davis, T. N. (1992). Calmodulin concentrates at regions of cell growth in Saccharomyces cerevisiae. J. Cell Biol. 118, 619-629.
4. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
5. Byers, B. (1981). Cytology of the Yeast Life Cycle. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Byers, B. and Goetsch, L. (1975). Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae. J. Bacteriol. 124, 511-523.
7. Cohen, P. and Klee, C. B. (1988). Calmodulin. Elsevier Science Publishers BV, Amsterdam, The Netherlands.
8. Davis, T. N. (1992). Mutational analysis of calmodulin in Saccharomyces cerevisiae. Cell Calcium 13, 435-444.
9. Davis, T. N., Urdea, M. S., Masiarz, F. R. and Thorner, J. (1986). Isolation of the yeast calmodulin gene: Calmodulin is an essential protein. Cell 47, 423-431.
10. Donaldson, A. D. and Kilmartin, J. V. (1996). Spc42p: Phosphorylated component of the S. cerevisiae spindle pole body (SPB) with an essential function during SPB duplication. J. Cell Biol. 132, 887-901.
11. Geiser, J. R., Sundberg, H. A., Chang, B. H., Muller, E. G. D. and Davis, T. N. (1993). The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 7913-7924.
12. Geiser, J. R., Tuinen, V. D., Brockerhoff, S. E., Neff, M. M. and Davis, T. N. (1991). Can calmodulin function without binding calcium? Cell 65, 949-959.
13. Geissler, S., Pereira, G., Spang, A., Knop, M., Souès, S., Kilmartin, J. and Schiebel, E. (1996). The spindle pole body component Spc98p interacts with the γ-tubulin-like Tub4p of S. cerevisiae at the sites of microtubule attachment. EMBO J. (in press).
14. Guthrie, C. and Fink, G. R. (1991). Guide to Yeast Genetics and Molecular Biology. Academic Press, New York.
15. Harlow, E. and Lane, D. (1988). Antibodies. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Kilmartin, J. V., Dyos, S. L., Kershaw, D. and Finch, J. T. (1993). A spacer protein in the Saccharomyces cerevisiae spindle pole body whose transcription is cell-cycle regulated. J. Cell Biol. 123, 1175-1184.
17. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
18. Mirzayan, C., Copeland, C. S. and Snyder, M. (1992). The NUFl gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeleton. J. Cell Biol. 116, 1319-1332.
19. Ohya, Y. and Botstein, D. (1994). Diverse essential functions revealed by complementing yeast calmodulin mutants. Science 263, 963-966.
20. O'Neil, K. T., Wolfe, H. R., Erickson-Viitanen, J. S. and Degrado, W. F. (1987). Fluorescence properties of calmodulin-binding peptides reflect alpha-helical periodicity. Science 236, 1454-1456.
21. Preuss, D., Mulholland, J., Kaiser, C. A., Orlean, P., Albright, C., Rose, M. D., Robbins, P. W. and Botstein, D. (1991). Structure of the yeast endoplasmic reticulum: Localization of ER proteins using immunofluorescence and immunoelectron microscopy. Yeast 7, 891-911.
22. Rothstein, R. J. (1983). One-step gene disruption in yeast. Meth. Enzymol. 101, 202-211.
23. Rout, M. P. and Kilmartin, J. V. (1990). Components of the yeast spindle and spindle pole body. J. Cell Biol. 111, 1913-1927.
24. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor.
25. Scatchard, G. (1949). The attractions of proteins for small molecules ard ions. Ann. NY Acad. Sci. 51, 660-672.
26. Schulman, H., Hanson, P. I. and Meyer, T. (1992). Decoding calcium signals by multifunctional CAM kinase. Cell Calcium 13, 401-411.
27. Sikorski, R. S. and Hieter, P. (1989). A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27.
28. Spang, A., Courtney, I., Fackler, U., Matzner, M. and Schiebel, E. (1993). The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole body. J. Cell Biol. 123, 405-416.
29. Spang, A., Courtney, I., Grein, K., Matzner, M. and Schiebel, E. (1995). The Cdc31 p-binding protein Kar1p is a component of the half-bridge of the yeast spindle pole body. J. Cell Biol. 128, 863-877.
30. Spang, A., Geissler, S., Grein, K. and Schiebel, E. (1996). The γ-tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation. J. Cell Biol., in press.
31. Stirling, D. A., Welch, K. A. and Stark, M. J. R. (1994). Interaction with calmodulin is required for the function of Spc110p, an essential component of the yeast spindle pole body. EMBO 13, 4329-4342.
32. Studier, F. W. and Moffat, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes. J. Mol. Biol. 189, 113-130.
33. Tabor, S. and Richardson, C. C. (1985). A bacteriophage T7 RNA polymerase/promotor system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. 82, 1074-1078.
34. Yano, K., Araki, Y., Hales, S. J., Tanaka, M. and Ikebe, M. (1993). Boundary of the autoinhibitory region of smooth-muscle myosin light-chain kinase. Biochemistry 32, 12054-12061.