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10
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Baca, M.1
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0029140496
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L. E. Canne, A. R. Ferre-D'Amare, S. K. Burley, and S. B. H. Kent, J. Am. Chem. Soc. 117, 2998 (1992).
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Canne, L.E.1
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12
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0027944205
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P. E. Dawson, T. W. Muir, I. Clark-Lewis, and S. B. H. Kent, Science 266, 776 (1994).
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Dawson, P.E.1
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84939246733
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T. Wieland, E. Bokelmann, L. Bauer, H. U. Lang, and H. Lau, Liebigs Ann. Chem. 583, 129 (1953).
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Wieland, T.1
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Lau, H.5
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14
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0030973396
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P. E. Dawson, M. Churchill, R. M. Ghadiri, and S. B. H. Kent, J. Am. Chem. Soc. 119, 4325 (1997).
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Dawson, P.E.1
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Kent, S.B.H.4
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15
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85030300443
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note
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12
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16
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0029559773
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Phosphines have also been added during ligation as a reducing agent. J. P. Tam, Proc. Natl. Acad. Sci. U.S.A. 92, 12485 (1995).
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(1995)
Proc. Natl. Acad. Sci. U.S.A.
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Tam, J.P.1
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17
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0030037155
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A promising extension has been reported that permits Gly-Xaa and Xaa-Gly sites to be utilized. This extension has not yet been applied to proteins [L. E. Canne, S. J. Bark, and S. B. H. Kent, J. Am. Chem. Soc. 118, 55891 (1996)].
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J. Am. Chem. Soc.
, vol.118
, pp. 55891
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Canne, L.E.1
Bark, S.J.2
Kent, S.B.H.3
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21
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85030305272
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poster presented San Francisco, California, October
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M. A. Siani, D. A. Thompson, L. E. Canne, G. M. Figliozzi, B. Robson, S. B. H. Kent, and R. J. Simon, poster presented at the IB3 Chemokines 3 Conference, San Francisco, California, October 1996
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(1996)
IB3 Chemokines 3 Conference
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Siani, M.A.1
Thompson, D.A.2
Canne, L.E.3
Figliozzi, G.M.4
Robson, B.5
Kent, S.B.H.6
Simon, R.J.7
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22
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0026486811
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M. Schnolzer, P. Alewood, D. Alewood, and S. B. H. Kent, Int. J. Pept. Protein Res. 85, 180 (1992).
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Schnolzer, M.1
Alewood, P.2
Alewood, D.3
Kent, S.B.H.4
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23
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85030301979
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note
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Fmoc chemistry has not been utilized because of the susceptibility of thioester peptides to aminolysis by piperidine. Use of a resin designed to be cleaved by nucleophiles following synthesis may allow thioester peptides to be synthesized by Fmoc protocols.
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-
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24
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85030306605
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note
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Small amounts of alkylation of the tryptophan side chain are observed during synthesis. In principle, the formyl protecting group could be removed either following or during ligation.
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25
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12644270357
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personal communication
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T. Hackeng, personal communication (1996).
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(1996)
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Hackeng, T.1
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28
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0000162728
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A. R. Mitchell, S. B. H. Kent, B. W. Erickson, and R. B. Merifield, Tetrahedron Lett. 42, 3795 (1976).
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(1976)
Tetrahedron Lett.
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Mitchell, A.R.1
Kent, S.B.H.2
Erickson, B.W.3
Merifield, R.B.4
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30
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0019627519
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V. K. Sarin, S. B. H. Kent, J. P. Tam, and R. B. Merrifield, Anal. Biochem. 117, 147 (1981).
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(1981)
Anal. Biochem.
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Sarin, V.K.1
Kent, S.B.H.2
Tam, J.P.3
Merrifield, R.B.4
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31
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85030301869
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note
-
The alkylation reaction can also be performed using benzyl bromide as the alkylating agent, producing a benzyl thioester peptide. Complications can arise owing to the poor solubility of benzyl mercaptan in aqueous solutions and its greater reactivity toward the peptide side chains.
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32
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0001462324
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H. Hojo, Y. Kwon, Y. Kakuta, S. Tsuda, I. Tanaka, K. Hikichi, and S. Aimoto, Bull. Chem. Soc. Jpn. 66, 2700 (1993).
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Bull. Chem. Soc. Jpn.
, vol.66
, pp. 2700
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Hojo, H.1
Kwon, Y.2
Kakuta, Y.3
Tsuda, S.4
Tanaka, I.5
Hikichi, K.6
Aimoto, S.7
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33
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85030306547
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note
-
During ligation, a white precipitate may form in the ligation mixture. This is a result of oxidation of thiophenol and benzyl mercaptan and can be removed after ligation by filtration.
-
-
-
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34
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12644298818
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personal communication
-
M. C. Fitzgerald, personal communication (1996).
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(1996)
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Fitzgerald, M.C.1
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