Phospholipase A2 engineering. Structural and functional roles of the highly conserved active site residue aspartate-99

Biochemistry

Volumn 36, Issue 11, 1997, Pages 3104-3114

  Sekar, Kanagaraj ^a   Yu, Bao Zhu ^b   Rogers, Joseph ^b   Lutton, John ^c   Liu, Xiaohong ^a   Chen, Xin ^a   Tsai, Ming Daw ^a   Jain, Mahendra K ^b   Sundaralingam, Muttaiya ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

^c KENYON COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHOSPHOLIPASE A2; ASPARTIC ACID; PHOSPHOLIPASE A; RECOMBINANT PROTEIN;

ARTICLE; DENATURATION; ELECTRON TRANSPORT; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME KINETICS; PRIORITY JOURNAL; PROTEIN ANALYSIS; STRUCTURE ACTIVITY RELATION; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; COMPARATIVE STUDY; COMPUTER SIMULATION; ENZYME STABILITY; ENZYMOLOGY; HYDROGEN BOND; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; NUCLEOTIDE SEQUENCE; PANCREAS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; BINDING SITES; CATTLE; COMPUTER SIMULATION; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; HYDROGEN BONDING; KINETICS; MODELS, MOLECULAR; PANCREAS; PHOSPHOLIPASES A; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 0030615005     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi961576x     Document Type: Article

References (48)

1. Annand, R. R., Kontoyianni, M., Penzoth, J. E., Dudler, T., Leybrand, T. P., & Gelb, M. H. (1996) Biochemistry 35, 4591-4601.
2. Berg, O. G., Yu, B.-Z., Rogers, J., & Jain, M. K. (1991) Biochemistry 30, 7283-7297.
3. Brunger, A. T. (1992) X-PLOR Manual, Yale University, New Haven, CT.
4. Cajal, Y., Rogers, J., Berg, O. G., & Jain, M. K. (1996) Biochemistry 35, 299-308.
5. DeHaas, G. H., Bonsen, P. P. M., Pieterson, W. A., & Van Seenen, L. L. M. (1971) Biochem. Biophys. Acta 239, 252-266.
6. Dijkstra, B. W., Drenth, J., & Kalk, K. H. (1981a) Nature 289, 604-606.
7. Dijkstra, B. W., Kalk, K. H., Hol, W. G. J., & Drenth, J. (1981b) J. Mol. Biol. 147, 97-123.
8. Dijkstra, B. W., Renetseder, R., Kalk, K. H., Hol, W. G. J., & Drenth, J. (1983) J. Mol. Biol. 1681, 63-179.
9. Dupureur, C. M., Yu, B.-Z., Jain, M. K., Noel, J. P., Deng, T., Li, Y., Byeon, I. L., & Tsai, M.-D. (1992a) Biochemistry 31, 6402-6413.
10. Dupureur, C. M., Yu, B.-Z., Memone, J. A., Jain, M. K., & Tsai, M-D. (1992b) Biochemistry 31, 10576-10583.
11. Huang, B., Yu, B.-Z., Rogers, J., Byeon, I.-J. L., Sekar. K., Chen. K., Sundaralingam, M., Jain, M. K., & Tsai, M.-D. (1996) Biochemistry 35, 12164-12174.
12. Jain, M. K., & Vaz, W. L. C. (1987) Biochim. Biophys. Acta 905, 1-8.
13. Jain, M. K., & Maliwal, B. P. (1993) Biochemistry 32, 11838-11846.
14. Juin, M. K., Egmond, M. R., Verheij, H. M., Apitz-Castro, R. J., Dijkman, R., & de Haas, G. H. (1982) Biochim. Biophys. Acta 688, 341-348.
15. Jain, M. K., Rogers, J., Jahagirdar, D. V., Marecek, J. F., & Ramirez, F. (1986a) Biochim. Biophys. Acta 860, 435-447.
16. Jain, M. K., Maliwal, B. P., de Haas, G. H., & Slotboom, A. J. (1986b) Biochim. Biophys. Acta 860, 448-461.
17. Jain, M. K., Rogers, J., Marecek, J. F., Ramirez, F., & Eibl, H. (1986c) Biochim. Biophys. Acta 860, 462-474.
18. Jain, M. K., Rogers, J., & de Haas, G. H. (1988) Biochim. Biophys. Acta 940, 51-62.
19. Jain, M. K., Yu, B.-Z., Rogers, J., Ranadive, G. N., & Bera, O. G. (1991a) Biochemistry 30, 7306-7317.
20. Jain, M. K., Rogers, J., Berg, O., & Gelb, M. H. (1991b) Biochemistry 30, 7340-7348.
21. Jain, M. K., Tao, W., Rogers, J., Arenson, C., Eibl, H., & Yu, B.-Z. (1991c) Biochemistry 30, 10256-10268.
22. Jain, M. K., Yu, B.-Z., & Bers, O. G. (1993) Biochemistry 32, 11319-11329.
23. Jain, M. K., Gelb, M. H., Rogers, J., & Berg, O. G. (1995) Methods Enzymol. 249, 567-614.
24. Jain, M. K., Rogers, J., Yu, B.-Z., Yao, J., & Romsted, L. S. (1997) (to be submitted for publication).
25. Jones, T. A. (1985) Methods Enzymol, 115, 157-171.
26. Kuipers, O. P., Franken, P. A., Hendricks, R., Verheij, H. M., & de Haas, G. H. (1990) Protein Eng. 4, 199-204.
27. Kuipers, O. P., Vincent, M., Brochon, J., Verheij, H. M., de Haas, G. H., & Gallay, J. (1991) Biochemistry 30, 8771-8785.
28. Kumar, A., Sekharudu, Y. C., Ramakrishnan, B., Dupureur, C. M., Zhu, H., Tsai, M.-D., & Sundaralingam, M. (1994) Protein Sci. 3, 2082-2088.
29. Laskowski, R. A., MacArthur, M. W., Moss, D. S., & Thornton, J. M. (1993) J. Appl. Crystallogr. 26, 283-291.
30. Li, Y., & Tsai, M.-D. (1993) J. Am. Chem. Soc. 115, 8523-8326.
31. Li, Y., Yu, B.-Z., Zhu, H., Jain, M. K., & Tsai, M.-D. (1994) Biochemistry 33, 14714-14722.
32. Liu, X., Zhu, H., Huang, B., Yu, B.-Z., Rogers, J., Kumar, A., Jain, M. K., Sundaralingam, M., & Tsai, M.-D. (1995) Biochemistry 34, 7322-7334.
33. Luzzati, V. (1952) Acta Crystallogr. 5, 802-807.
34. Maliwal, B. P., Yu, B. Z., Szacinski, H., Squier, T., van Binsbergen, J., Slotboom, A. J., & Jain, M. K. (1994) Biochemistry 33, 4509-4516.
35. Noel, J. P., Bingman, C., Deng, T., Dupureur, C. M., Hamilton, K. J., Jiang, R.-T., Kwak, J.-G., Sekharudu, Y. C., Sundaralingam, M., & Tsai, M.-D. (1991) Biochemistry 30, 11801-11811.
36. Pieterson, W. A., Vidal, J. C., Volwerk, J. J., & de Haas, G. H. (1974) Biochemistry 13, 1455-1460.
37. Ramirez, F., & Jain, M. K. (1991) Proteins 9, 229-239.
38. Rogers, J., Yu, B. Z., Serves, S. V., Tsivgoulis, G. M., Sotivopoulos, D. N., Ioannou, P. V., & Jain, M. K. (1996) Biochemistry 35, 9375-9385.
39. Scott, D. L., White, S. P., Otwinowski, Z., Yuan, W., Gelb, M. H., & Sigler, P. B. (1990a) Science 250, 1541-1546.
40. Scott, D. L., Otwinowsi, Z., Gelb, M. H., & Sigler, P. B. (1990b) Science 250, 1563-1566.
41. Sekharudu, Y. C., Ramakrishnan, B., Huang, B., Jiang, R.-T., Dupureur, C. M., Tsai, M.-D., & Sundaralingam. M. (1992) Protein Sci. 1, 1585-1594.
42. Seshadri, K., Vishveshwara, S., & Jain, M. K. (1994) Proc. Indian Acad. Sci. 106, 1177-1189.
43. Thunnissen, M. M. G. M., Ab, E., Kalk, K. H., Drench, J., Dijkstra, B. W., Kuipers, O. P., Dijkman, R., de Haas, G. H., & Verheij, H. M. (1990) Nature 347, 689-691.
44. Van den Berg, B., Tessari, M., Boelens, R., Dijkman, R., Kaptein, R., de Haas, G. H., & Verheij, H. M. (1995) J. Biomol. NMR 5, 110-121.
45. Verheij, H. M., Slotboom, A. J., & de Haas, G. H. (1981) Rev. Physiol. Biochem. Pharmacol. 91, 91-203.
46. Volwerk, J. J., & de Haas, G. H. (1982) in Lipid Protein Interactions (Jost, P. C., & Griffith, O. H., Eds.) Vol. 1, pp 69-149, John Wiley & Sons, New York.
47. Weis, W. I., Drikamer, K., & Hendrickson, W. A. (1992) Nature 360, 127-134.
48. Yu, B.-Z., Berg, O. G., & Jain, M. K. (1993) Biochemistry 32, 6485-6492.