Phospholipase A2 engineering. Deletion of the C-terminus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic interface

Biochemistry

Volumn 35, Issue 37, 1996, Pages 12164-12174

  Huang, Baohua ^a,c   Yu, Bao Zhu ^b   Rogers, Joseph ^b   Byeon, In Ja L ^a   Sekar, Kanagaraj ^a   Chen, Xin ^a   Sundaralingam, Muttaiya ^a   Tsai, Ming Daw ^a   Jain, Mahendra K ^b  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF DELAWARE   (United States)

^c ABBOTT LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPHOLYTE; PHOSPHOLIPASE A2; CALCIUM; PHOSPHOLIPASE A; PRIMER DNA; RECOMBINANT PROTEIN;

ARTICLE; BINDING SITE; CALCIUM BINDING; CARBOXY TERMINAL SEQUENCE; DELETION MUTANT; DISSOCIATION CONSTANT; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SPECIFICITY; GENE DELETION; GENETIC ENGINEERING; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CALORIMETRY; CATTLE; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER GRAPHICS; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; METABOLISM; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PANCREAS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; SENSITIVITY AND SPECIFICITY; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CALCIUM; CALORIMETRY; CATTLE; CLONING, MOLECULAR; COMPUTER GRAPHICS; DNA PRIMERS; ENZYME STABILITY; ESCHERICHIA COLI; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PANCREAS; PHOSPHOLIPASES A; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE DELETION; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

EID: 0029661921     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi960234o     Document Type: Article

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