Regulation of NF-κB by cyclin-dependent kinases associated with the p300 coactivator

Science

Volumn 275, Issue 5299, 1997, Pages 523-527

  Perkins, Neil D ^a,c   Felzien, Lisa K ^a   Betts, Jonathan C ^a   Leung, Kwanyee ^a   Beach, David H ^b   Nabel, Gary J ^a  

^a UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; TRANSCRIPTION FACTOR; CDK2 PROTEIN, HUMAN; CDKN1A PROTEIN, HUMAN; CYCLIN DEPENDENT KINASE 2; CYCLIN DEPENDENT KINASE INHIBITOR 1A; CYCLINE; HISTONE H1 KINASE; NUCLEAR PROTEIN; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE; PROTEIN SERINE THREONINE KINASE; TRANSACTIVATOR PROTEIN; TRANSCRIPTION FACTOR RELA;

ARTICLE; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION; CELL CYCLE; CELL LINE; GENETIC TRANSFECTION; GENETICS; LEUKEMIA CELL LINE; METABOLISM; PHOSPHORYLATION; REPORTER GENE; SIGNAL TRANSDUCTION; TRANSACTIVATION;

CDC2-CDC28 KINASES; CELL CYCLE; CELL LINE; CYCLIN-DEPENDENT KINASE 2; CYCLIN-DEPENDENT KINASE INHIBITOR P21; CYCLIN-DEPENDENT KINASES; CYCLINS; GENES, REPORTER; HUMANS; JURKAT CELLS; NF-KAPPA B; NUCLEAR PROTEINS; PHOSPHORYLATION; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; SIGNAL TRANSDUCTION; TETRADECANOYLPHORBOL ACETATE; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTOR RELA; TRANSCRIPTION FACTORS; TRANSFECTION;

EID: 0030614504     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5299.523     Document Type: Article

References (67)

1. R. W. King, P. K. Jackson, M. W. Kirschner, Cell 79, 563 (1994); K. A. Heichman and J. M. Roberts, ibid., p. 557; C. J. Sherr, ibid., p. 551; P. Nurse, ibid., p. 547.
2. R. W. King, P. K. Jackson, M. W. Kirschner, Cell 79, 563 (1994); K. A. Heichman and J. M. Roberts, ibid., p. 557; C. J. Sherr, ibid., p. 551; P. Nurse, ibid., p. 547.
3. R. W. King, P. K. Jackson, M. W. Kirschner, Cell 79, 563 (1994); K. A. Heichman and J. M. Roberts, ibid., p. 557; C. J. Sherr, ibid., p. 551; P. Nurse, ibid., p. 547.
4. R. W. King, P. K. Jackson, M. W. Kirschner, Cell 79, 563 (1994); K. A. Heichman and J. M. Roberts, ibid., p. 557; C. J. Sherr, ibid., p. 551; P. Nurse, ibid., p. 547.
5. T. Hunter and J. Pines, ibid., p. 573; M. Peter and I. Herskowitz, ibid., p. 181.
6. T. Hunter and J. Pines, ibid., p. 573; M. Peter and I. Herskowitz, ibid., p. 181.
7. Y. Gu, C. W. Turck, D. O. Morgan, Nature 366, 707 (1993); J. W. Harper, G. R. Adami, N. Wei, K. Keyomarsi, S. J. Bledge, Cell 75, 805 (1993); A. Noda, Y. Ning, S. F. Venable, O. M. Pereira-Smith, J. R. Smith, Exp. Cell Res. 211, 90 (1994).
8. Y. Gu, C. W. Turck, D. O. Morgan, Nature 366, 707 (1993); J. W. Harper, G. R. Adami, N. Wei, K. Keyomarsi, S. J. Bledge, Cell 75, 805 (1993); A. Noda, Y. Ning, S. F. Venable, O. M. Pereira-Smith, J. R. Smith, Exp. Cell Res. 211, 90 (1994).
9. Y. Gu, C. W. Turck, D. O. Morgan, Nature 366, 707 (1993); J. W. Harper, G. R. Adami, N. Wei, K. Keyomarsi, S. J. Bledge, Cell 75, 805 (1993); A. Noda, Y. Ning, S. F. Venable, O. M. Pereira-Smith, J. R. Smith, Exp. Cell Res. 211, 90 (1994).
10. Y. Xiong et al., Nature 366, 701 (1993).
11. W. S. El-Deiry et al., Cell 75, 817 (1993); Y. Li, C. W. Jenkins, M. A. Nichols, Y. Xiong, Oncogene 9, 2261 (1994).
12. W. S. El-Deiry et al., Cell 75, 817 (1993); Y. Li, C. W. Jenkins, M. A. Nichols, Y. Xiong, Oncogene 9, 2261 (1994).
13. W. S. El-Deiry et al., Cancer Res. 54, 1169 (1994); A. Di Leonardo, S. P. Linke, K. Clarkin, G. M. Wahl, Genes Dev. 8, 2540 (1994); V. Dulic et al., Cell 76, 1013 (1994).
14. W. S. El-Deiry et al., Cancer Res. 54, 1169 (1994); A. Di Leonardo, S. P. Linke, K. Clarkin, G. M. Wahl, Genes Dev. 8, 2540 (1994); V. Dulic et al., Cell 76, 1013 (1994).
15. W. S. El-Deiry et al., Cancer Res. 54, 1169 (1994); A. Di Leonardo, S. P. Linke, K. Clarkin, G. M. Wahl, Genes Dev. 8, 2540 (1994); V. Dulic et al., Cell 76, 1013 (1994).
16. H. Jiang et al., Oncogene 9, 3397 (1994); R. A. Steinman et al., ibid., p. 3389.
17. H. Jiang et al., Oncogene 9, 3397 (1994); R. A. Steinman et al., ibid., p. 3389.
18. P. Michieli et al., Cancer Res. 54, 3391 (1994).
19. R. Sen and D. Baltimore, Cell 47, 921 (1986); C. Thevenin et al., New Biol. 2, 793 (1990); A. S. Baldwin, J. C. Azizkhan, D. E. Jensen, A. A. Beg, L. R. Coodly, Mol. Cell. Biol. 11, 4943 (1991); Y. Devary, C. Rosette, J. A. DiDonato, M. Karin, Science 261, 1442 (1993).
20. R. Sen and D. Baltimore, Cell 47, 921 (1986); C. Thevenin et al., New Biol. 2, 793 (1990); A. S. Baldwin, J. C. Azizkhan, D. E. Jensen, A. A. Beg, L. R. Coodly, Mol. Cell. Biol. 11, 4943 (1991); Y. Devary, C. Rosette, J. A. DiDonato, M. Karin, Science 261, 1442 (1993).
21. R. Sen and D. Baltimore, Cell 47, 921 (1986); C. Thevenin et al., New Biol. 2, 793 (1990); A. S. Baldwin, J. C. Azizkhan, D. E. Jensen, A. A. Beg, L. R. Coodly, Mol. Cell. Biol. 11, 4943 (1991); Y. Devary, C. Rosette, J. A. DiDonato, M. Karin, Science 261, 1442 (1993).
22. R. Sen and D. Baltimore, Cell 47, 921 (1986); C. Thevenin et al., New Biol. 2, 793 (1990); A. S. Baldwin, J. C. Azizkhan, D. E. Jensen, A. A. Beg, L. R. Coodly, Mol. Cell. Biol. 11, 4943 (1991); Y. Devary, C. Rosette, J. A. DiDonato, M. Karin, Science 261, 1442 (1993).
23. G. P. Nolan and D. Baltimore, Curr. Opin. Genet. Dev. 2, 211 (1992); M. Grilli, J. J. Chiu, M. J. Lenardo, Int. Rev. Cytol. 143, 1 (1993); U. Siebenlist, G. Franzoso, K. Brown, Annu. Rev. Cell Biol. 10, 405 (1994).
24. G. P. Nolan and D. Baltimore, Curr. Opin. Genet. Dev. 2, 211 (1992); M. Grilli, J. J. Chiu, M. J. Lenardo, Int. Rev. Cytol. 143, 1 (1993); U. Siebenlist, G. Franzoso, K. Brown, Annu. Rev. Cell Biol. 10, 405 (1994).
25. G. P. Nolan and D. Baltimore, Curr. Opin. Genet. Dev. 2, 211 (1992); M. Grilli, J. J. Chiu, M. J. Lenardo, Int. Rev. Cytol. 143, 1 (1993); U. Siebenlist, G. Franzoso, K. Brown, Annu. Rev. Cell Biol. 10, 405 (1994).
26. G. E. Griffin, K. Leung, T. M. Folks, S. Kunkel, G. J. Nabel, Nature 339, 70 (1989).
27. S. L. Eck, N. D. Perkins, D. P. Carr, G. J. Nabel, Mol. Cell. Biol. 13, 6530 (1993).
28. G. Nabel and D. Baltimore, Nature 326, 711 (1987).
29. C. S. Duckett et al., Mol. Cell. Biol. 13, 1315 (1993).
30. M. Serrano, G. J. Hannon, D. Beach, Nature 366, 704 (1993).
31. G. J. Hannon and D. Beach, ibid. 371, 257 (1994); T. Suzuki, S. Kitao, H. Matsushime, M. Yoshida, EMBO J. 15, 1607 (1996).
32. G. J. Hannon and D. Beach, ibid. 371, 257 (1994); T. Suzuki, S. Kitao, H. Matsushime, M. Yoshida, EMBO J. 15, 1607 (1996).
33. N. D. Perkins, L. K. Felzien, G. J. Nabel, unpublished data.
34. K. Leung and G. J. Nabel, Nature 333, 776 (1988).
35. L. Osborn, S. Kunkel, G. J. Nabel, Proc. Natl. Acad. Sci. U.S.A. 86, 2336 (1989).
36. W. Lin and L. A. Culp, Biotechniques 11, 344 (1991); N. D. Perkins, A. B. Agranoff, C. S. Duckett, G. J. Nabel, J. Virol. 68, 6820 (1994).
37. W. Lin and L. A. Culp, Biotechniques 11, 344 (1991); N. D. Perkins, A. B. Agranoff, C. S. Duckett, G. J. Nabel, J. Virol. 68, 6820 (1994).
38. 32P]adenosine triphosphate (ATP) and 2 μg of purified histone H1 (Boehringer Mannheim) at 37°C for 15 min with frequent agitation. The reaction was terminated by the addition of SDS gel loading buffer, and a sample was resolved on an SDS-polyacrylamide (15%) gel, which was then dried and subjected to autoradiography. Cyclin-Cdk immunoprecipitations were done as described above for RelA, using 100 or 600 μg of nuclear extract and 10 μg of agarose-conjugated antibodies from Santa Cruz [sc-163AC (Cdk2), sc-601 AC (Cdk4), sc-054AC (Cdc2), sc-239AC (cyclin A), sc-245AC (cyclin B1), sc-246AC (cyclin D1), and sc-248AC (cyclin E)]. All antibodies to cyclins were mouse monoclonals (IgG1), the antibodies to Cdk2 and Cdk4 were rabbit polyclonal antibodies to Cdk peptides, and the antibody to Cdc2 was a mouse monoclonal (IgG2a). Protein immunoblotting of RelA was done with a rabbit polyclonal antibody (sc-109, Santa Cruz).
39. S. van den Heuvel and E. Harlow, Science 262, 2050 (1993).
40. Z. Arany, W. R. Sellers, D. M. Livingston, R. Eckner, Cell 77, 799 (1994).
41. 35S-labeled proteins (TNT rabbit coupled reticulocyte lysate system, Promega). Purified mouse IgG2b was used as the control. Protein association experiments, Bluescript RelA, RelA/VP16, Sp1, NF-κB1(p50), and pET RelA(1-306) plasmids used for in vitro transcription-translation have been described [N. D. Perkins, A. B. Agranoff, E. Pascal, G. J. Nabel, Mol. Cell. Biol. 14, 6570 (1994)].
42. J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. L. Harter, R. H. Goodman, Nature 374, 85 (1995); A. J. Bannister and T. Kouzarides, EMBO J. 14, 4758 (1995); R. Janknect and A. Nordheim, Oncogene 12, 1961 (1996); P. Dai et al., Genes Dev. 10, 528 (1996).
43. J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. L. Harter, R. H. Goodman, Nature 374, 85 (1995); A. J. Bannister and T. Kouzarides, EMBO J. 14, 4758 (1995); R. Janknect and A. Nordheim, Oncogene 12, 1961 (1996); P. Dai et al., Genes Dev. 10, 528 (1996).
44. J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. L. Harter, R. H. Goodman, Nature 374, 85 (1995); A. J. Bannister and T. Kouzarides, EMBO J. 14, 4758 (1995); R. Janknect and A. Nordheim, Oncogene 12, 1961 (1996); P. Dai et al., Genes Dev. 10, 528 (1996).
45. J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. L. Harter, R. H. Goodman, Nature 374, 85 (1995); A. J. Bannister and T. Kouzarides, EMBO J. 14, 4758 (1995); R. Janknect and A. Nordheim, Oncogene 12, 1961 (1996); P. Dai et al., Genes Dev. 10, 528 (1996).
46. P. Yaciuk and E. Moran, Mol. Cell. Biol. 11, 5389 (1991); S. E. Abraham, S. Lobo, P. Yaciuk, H. H. Wang, E. Moran, Oncogene 8, 1639 (1993); R. Eckner et al., Genes Dev. 8, 869 (1994); A. C. Banerjee et al., Oncogene 9, 1733 (1994); C. Missero et al., Proc. Natl. Acad. Sci. U.S.A. 92, 5451 (1995).
47. P. Yaciuk and E. Moran, Mol. Cell. Biol. 11, 5389 (1991); S. E. Abraham, S. Lobo, P. Yaciuk, H. H. Wang, E. Moran, Oncogene 8, 1639 (1993); R. Eckner et al., Genes Dev. 8, 869 (1994); A. C. Banerjee et al., Oncogene 9, 1733 (1994); C. Missero et al., Proc. Natl. Acad. Sci. U.S.A. 92, 5451 (1995).
48. P. Yaciuk and E. Moran, Mol. Cell. Biol. 11, 5389 (1991); S. E. Abraham, S. Lobo, P. Yaciuk, H. H. Wang, E. Moran, Oncogene 8, 1639 (1993); R. Eckner et al., Genes Dev. 8, 869 (1994); A. C. Banerjee et al., Oncogene 9, 1733 (1994); C. Missero et al., Proc. Natl. Acad. Sci. U.S.A. 92, 5451 (1995).
49. P. Yaciuk and E. Moran, Mol. Cell. Biol. 11, 5389 (1991); S. E. Abraham, S. Lobo, P. Yaciuk, H. H. Wang, E. Moran, Oncogene 8, 1639 (1993); R. Eckner et al., Genes Dev. 8, 869 (1994); A. C. Banerjee et al., Oncogene 9, 1733 (1994); C. Missero et al., Proc. Natl. Acad. Sci. U.S.A. 92, 5451 (1995).
50. P. Yaciuk and E. Moran, Mol. Cell. Biol. 11, 5389 (1991); S. E. Abraham, S. Lobo, P. Yaciuk, H. H. Wang, E. Moran, Oncogene 8, 1639 (1993); R. Eckner et al., Genes Dev. 8, 869 (1994); A. C. Banerjee et al., Oncogene 9, 1733 (1994); C. Missero et al., Proc. Natl. Acad. Sci. U.S.A. 92, 5451 (1995).
51. A. M. Ventura, M. Q. Arens, A. Srinivasan, G. Chinnadurai, Proc. Natl. Acad. Sci. U.S.A. 87, 1310 (1990); H. G. Wang et al., J. Virol. 67, 476 (1993); C. Song, P. M. Loewenstein, M. Green, ibid. 69, 2907 (1995); S. F. Parker, L. K. Felzien, N. D. Perkins, M. J. Imperiale, G. J. Nabel, J. Virol., in press.
52. A. M. Ventura, M. Q. Arens, A. Srinivasan, G. Chinnadurai, Proc. Natl. Acad. Sci. U.S.A. 87, 1310 (1990); H. G. Wang et al., J. Virol. 67, 476 (1993); C. Song, P. M. Loewenstein, M. Green, ibid. 69, 2907 (1995); S. F. Parker, L. K. Felzien, N. D. Perkins, M. J. Imperiale, G. J. Nabel, J. Virol., in press.
53. A. M. Ventura, M. Q. Arens, A. Srinivasan, G. Chinnadurai, Proc. Natl. Acad. Sci. U.S.A. 87, 1310 (1990); H. G. Wang et al., J. Virol. 67, 476 (1993); C. Song, P. M. Loewenstein, M. Green, ibid. 69, 2907 (1995); S. F. Parker, L. K. Felzien, N. D. Perkins, M. J. Imperiale, G. J. Nabel, J. Virol., in press.
54. A. M. Ventura, M. Q. Arens, A. Srinivasan, G. Chinnadurai, Proc. Natl. Acad. Sci. U.S.A. 87, 1310 (1990); H. G. Wang et al., J. Virol. 67, 476 (1993); C. Song, P. M. Loewenstein, M. Green, ibid. 69, 2907 (1995); S. F. Parker, L. K. Felzien, N. D. Perkins, M. J. Imperiale, G. J. Nabel, J. Virol., in press.
55. R. Roy et al., Cell 79, 1093 (1994); R. Shiekhattar et al., Nature 374, 283 (1995); S.-M. Liao et al., ibid., p. 193; H.-P. Gerber et al., ibid., p. 660.
56. R. Roy et al., Cell 79, 1093 (1994); R. Shiekhattar et al., Nature 374, 283 (1995); S.-M. Liao et al., ibid., p. 193; H.-P. Gerber et al., ibid., p. 660.
57. R. Roy et al., Cell 79, 1093 (1994); R. Shiekhattar et al., Nature 374, 283 (1995); S.-M. Liao et al., ibid., p. 193; H.-P. Gerber et al., ibid., p. 660.
58. R. Roy et al., Cell 79, 1093 (1994); R. Shiekhattar et al., Nature 374, 283 (1995); S.-M. Liao et al., ibid., p. 193; H.-P. Gerber et al., ibid., p. 660.
59. T. D. Gilmore and H. M. Temin, Cell 44, 791 (1986); A. Neri et al., ibid. 67, 1075 (1991).
60. T. D. Gilmore and H. M. Temin, Cell 44, 791 (1986); A. Neri et al., ibid. 67, 1075 (1991).
61. F. Petrij et al., Nature 376, 348 (1995); M. Muraoka et al., Oncogene 12, 1565 (1996).
62. F. Petrij et al., Nature 376, 348 (1995); M. Muraoka et al., Oncogene 12, 1565 (1996).
63. J. He et al., J. Virol. 69, 6705 (1995); J. B. M. Jowett et al., ibid., p. 6304; S. R. Bartz, M. E. Rogel, M. Emerman, ibid. 70, 2324 (1996).
64. J. He et al., J. Virol. 69, 6705 (1995); J. B. M. Jowett et al., ibid., p. 6304; S. R. Bartz, M. E. Rogel, M. Emerman, ibid. 70, 2324 (1996).
65. J. He et al., J. Virol. 69, 6705 (1995); J. B. M. Jowett et al., ibid., p. 6304; S. R. Bartz, M. E. Rogel, M. Emerman, ibid. 70, 2324 (1996).
66. II130, TFIIH p89 subunit, Cdk2, cyclin A, cyclin B1, and cyclin E were from Santa Cruz (sc-369, sc-204, sc-735, sc-736, sc-293, sc-163, sc-239AC, sc-245AC, and sc-248AC, respectively). The p300 cDNAs were obtained by polymerase chain reaction (PCR) amplified with the EXPAND PCR kit (Boehringer) from polyadenylated RNA (0.5 μg) from human spleen (Clontech) with oligo(dT) as a primer and reverse transcriptase (Stratagene). A 5′ fragment (sense, 5′-GCTAAGCTTCACCATGGCCGAGAATGTGGTGGAACCGGGGCCG-3′; antisense, 5′-CACAGATCTGATGCATCTTTCTTCCGCACTCTGTAC-3′), as well as a 3′ p300 fragment overlapping the internal Bgl II site (sense, 5′-ATCAGATCTGTGTCCTTCACCATGAGATCATCTGGC-3′; antisense, 5′-GCTAGATCTCTAGTGTATGTCTAGTGTACTCTGTGAGAGG-3′) were isolated and subcloned into pBluescript downstream of the T7 promoter.
67. We thank D. Gschwend for secretarial assistance; N. Barrett and K. Carter for preparation of figures; G. Hannon for p21 and p16 plasmids; P. Hobart (Vical Inc.) for the VCL-1012 CMV expression plasmid; G. Enders, J. LaBaer, and E. Harlow for the dominant negative Cdk2 plasmid; and W. Kong, Z. Yang, S. Parker, and other members of the Nabel lab for their advice and comments. N.D.P. is the recipient of a Scholar Award from the American Foundation for AIDS Research (AmFAR) in memory of James Hoese. Supported in part by NIH grant RO1 AI29179.