Overexpression of the TGFβ-regulated zinc finger encoding gene, TIEG, induces apoptosis in pancreatic epithelial cells

Journal of Clinical Investigation

Volumn 99, Issue 10, 1997, Pages 2365-2374

  Tachibana, Issei ^a   Imoto, Mami ^a   Adjei, Philip N ^b   Gores, Gregory J ^b   Subramaniam, Malayannan ^c   Spelsberg, Thomas C ^c   Urrutia, Raul ^a,c  

^a ST MARY S HOSPITAL   (United States)

^b Center for Basic Research in Digestive Diseases   (United States)

^c MAYO GRADUATE SCHOOL   (United States)

Author keywords

apoptosis; pancreas; transcription factor; transforming growth factor ; zinc finger

Indexed keywords

TRANSCRIPTION FACTOR; TRANSFORMING GROWTH FACTOR BETA; ZINC FINGER PROTEIN; DNA BINDING PROTEIN; HYBRID PROTEIN; TIEG PROTEIN, HUMAN; TIEG PROTEIN, RAT;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CELL GROWTH; CELL POPULATION; CELL PROLIFERATION; CELL TRANSFORMATION; CONTROLLED STUDY; EPITHELIUM CELL; ETIOLOGY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; IMMEDIATE EARLY GENE; NONHUMAN; PANCREAS CARCINOMA; PANCREAS CELL; PANCREAS DUCT; PRIORITY JOURNAL; RAT; ADULT; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY SPECIFICITY; BIOSYNTHESIS; CELL CULTURE; CELL DIVISION; CELL LINE; CHEMISTRY; CONSENSUS SEQUENCE; CYTOLOGY; DRUG EFFECT; EPITHELIUM; GENE EXPRESSION; GENE LIBRARY; GENETIC TRANSFECTION; GENETICS; MALE; MOLECULAR GENETICS; PANCREAS; PANCREAS TUMOR; PHYSIOLOGY; SEQUENCE HOMOLOGY;

ADULT; AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; CELL DIVISION; CELL LINE; CONSENSUS SEQUENCE; DNA-BINDING PROTEINS; EPITHELIAL CELLS; EPITHELIUM; GENE EXPRESSION; GENE LIBRARY; HUMANS; MALE; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; PANCREAS; PANCREATIC NEOPLASMS; RATS; RECOMBINANT FUSION PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRANSCRIPTION FACTORS; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA; TUMOR CELLS, CULTURED; ZINC FINGERS;

EID: 0030610532     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119418     Document Type: Article

References (52)

1. Guan, K.L. 1994. The mitogen activated protein kinase signal transduction pathway: from the cell surface to the nucleus. Cell. Signalling. 6:581-589.
2. Hill, C.S., and R. Treisman. 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell. 80:199-211.
3. Ruddon, R.W. 1995. Cell-cycle regulation and apoptosis. In Cancer Biology. Oxford University Press, New York. 386-401.
4. Raijman, I., and B. Levin. 1993. Exocrine tumors of the pancreas. In The Pancreas; Biology, Pathology, and Disease. V.L.W. Go, E.P. DiMagno, J.D. Gardner, E. Lebenthal, H.A. Reber, G.A. Scheele, editors. Raven Press, New York. 899-912.
5. Savito, F., P.L. Herrera, J. Huarte, A. Nichols, R. Montesano, L. Orci, and J.D. Vassalli. 1994. TGFβ1 influences the relative development of the exocrine and endocrine pancreas in vitro. Development (Camb.). 120:3451-3462.
6. Sarner, M. 1993. Pancreatitis definitions and classifications. In The Pancreas; Biology, Pathology, and Disease. V.L.W. Go, E.P. DiMagno, J.D. Gardner, E. Lebenthal, H.A. Reber, G.A. Scheele, editors. Raven Press, New York. 575-580.
7. Sanvito, F., A. Nichols, P.L. Herrera, J. Huarte, A. Wohlwend, J.D. Vassalli, and L. Orci. 1995. TGF-beta 1 overexpression in murine pancreas induces chronic pancreatitis and, together with TNF-alpha, triggers insulin-dependent diabetes. Biochem. Biophys. Res. Commun. 217:1279-1286.
8. Slater, S.D., R.C. Williamson, and C.S. Foster. 1995. Expression of transforming growth factor beta 1 in chronic pancreatitis. Digestion. 56:237-241.
9. Lee, M.S, D. Gu, L. Feng, S. Curriden, M. Arnush, T. Krahl, D. Gurushanthaiah, C. Wilson, D.L. Loskutoff, H. Fox, et al. 1995. Accumulation of extracellular matrix and developmental dysregulation in the pancreas by transgenic production of transforming growth factor-beta 1. Am. J Pathol. 147:42-52.
10. van Laethem, JL., J. Deviere, A. Resibois, F. Rickaert, P. Vertongen, H. Ohtani, M. Cremer, K. Miyazono, and P. Robberecht. 1995. Localization of transforming growth factor beta 1 and its latent binding protein in human chronic pancreatitis. Gastroenterology. 108:1873-1881.
11. Gress, T., F. Muller-Pillasch, H.P. Elsasser, M. Bachem, C. Ferrara, H. Weidenbach, M. Lerch, and G. Adler. 1994. Enhancement of transforming growth factor beta 1 expression in the rat pancreas during regeneration from caerulein-induced pancreatitis. Eur. J. Clin. Invest. 24:679-685.
12. Friess, H., Y. Yamanaka, M. Buchler, M. Ebert, H.G. Berger, L.I. Gold, and M. Korc. 1993. Enhanced expression of transforming growth factor beta isoforms in pancreatic cancer correlates with decreased survival. Gastroenterology. 105:1846-1856.
13. Friess, H., Y. Yamanaka, M. Buchler, H.G. Berger, M.S. Kobrin, R.L. Baldwin, and M. Korc. 1993. Enhanced expression of the type II transforming growth factor beta receptor in human pancreatic cancer cells without alteration of type III receptor expression. Cancer Res. 53:2704-2707.
14. Hahn, S.A., M. Schutte, A.T.M.S. Hoque, C.A. Moskaluk, L.T. da Costa, E. Rozenblum, C.L. Weinstein, A. Fischer, C.J. Yeo, R.H. Hruban, and S.E. Kern. 1996. DPC-4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science (Wash. DC). 271:350-353.
15. Mesa, K., B. Gebelein, T. Cook, and R. Urrutia. 1996. Identification and characterization of zinc finger encoding genes from the tumoral exocrine pancreatic cell line AR42J. Cancer Lett. 103:143-149.
16. Gebelein, B., K. Mesa, and R. Urrutia. 1996. A novel profile of expressed sequence tags for zinc finger encoding genes from the poorly differentiated exocrine pancreatic cell line AR4IP. Cancer Lett. 105:225-231.
17. Mesa, K., B. Gebelein, and R. Urrutia. 1996. A novel krüppel-like zinc finger encoding gene isolated from pancreas is regulated by cholecystokinin. Pancreas. 13:500a. (Abstr.)
18. Gebelein, B., K. Mesa, M. Imoto, and R. Urrutia. 1996. Characterization of MAZF: a Mitogen-Activated Zinc Finger gene encoding a novel member of the Krüppel-associated box subfamily of transcriptional represser proteins. Pancreas. 13:438a. (Abstr.)
19. Kreidberg, J.A., H. Sariola, J.M. Loring, M. Maeda, J. Pelletier, D. Housman, and R. Jaenisch. 1993. WT-1 is required for early kidney development. Cell. 74:679-691.
20. Miyagawa, K., J. Kent, A. Schedl, V. van Heyningen, and N.D. Hastie. 1994. Wilms' tumor - a case of disrupted development. J. Cell. Sci. 18:(Suppl.):1-5.
21. Subramaniam, M., S.A. Harris, M.J. Oursler, K. Rasmussen, B.L. Riggs, and T.C. Spelsberg. 1995. Identification of a novel TGFβ-regulated gene encoding a putative zinc finger protein in human osteoblasts. Nucleic Acids. Res. 23: 4907-4912.
22. Yingling, J.M., X.F. Wang, and C.H. Bassing. 1995. Signalling by the transforming growth factor-β receptors. Biochem. Biophys. Acta. 1242:115-136.
23. Cook, T., K. Mesa, and R. Urrutia. 1996. Three dynamin-encoding genes are differentially expressed in the developing rat brain. J. Neurochem. 67: 927-931.
24. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
25. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.). 227:680-685.
26. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:4350-4354.
27. Cook,T.A., K.J. Mesa, B.A. Gebelein, and R. Urrutia. 1996. Up-regulation of dynamin II expression during the acquisition of a mature pancreatic acinar phenotype. J. Histochem. Cytochem. 44:1373-1378.
28. Huet, C. 1994. Production of polyclonal antibodies in rabbits. In Cell Biology: A Laboratory Handbook. J.E. Celis, editor. Academic Press, San Diego, CA. 257-268.
29. Huet, C. 1994. Purification of immunoglobulins. In Cell Biology: A Laboratory Handbook. J.E. Cells, editor. Academic Press, San Diego, CA. 291-296.
30. Oberhammer, F.A., M. Pavelka, S. Sharma, R. Tiefenbacher, A.F. Rurchio, W. Bursch, and R. Schulte-Hermann. 1992. Induction of apoptosis in cultured hepatocytes and in regressing liver by transforming growth factor beta 1. Proc. Natl. Acad. Sci. USA. 89:5408-5412.
31. Patel, T., S.F. Bronk, and G.J. Gores. 1994. Increase of intracellular magnesium promotes glycodeoxychoiate-induced apoptosis in rat hepatocytes. J. Clin. Invest. 94:2183-2192.
32. Kwo, P., T. Patel, S.F. Bronk, and G.J. Gores. 1995. Nuclear serine protease activity contributes to bile acid-induced apoptosis in hepatocytes. Am. J. Physiol. 268:G613-G621.
33. Chen, Y.T, C. Holcomb, and H.P. Moore. 1993. Expression and localization of two low molecular weight GTP-binding proteins. Rab8 and Rab10. by epitope tag. Proc. Natl. Acad. Sci. USA. 90:6508-6512.
34. Urrutia, R., M. A. McNiven, J.P. Albanesi, D.B. Murphy, and B. Kachar. 1991. Purified kinesin promotes vesicle motility and induces active sliding between microtubules in vitro. Proc. Natl. Acad. Sci. USA. 88:6701-6705.
35. Baldwin, R.L., and M. Korc. 1993. Growth inhibition of human pancreatic carcinoma cells by transforming growth factor beta-1. Growth Factors. 8: 23-34.
36. Freeman, J.W., C.A. Mattingly, and W.E. Strodel. 1995. Increased tumorigenicity in the human pancreatic cell line MIAPaCa-2 is associated with an aberrant regulation of an IGF-1 autocrine loop and lack of expression of the TGF-beta type II receptor. J. Cell Physiol. 165:155-163.
37. Chesnut, J.D., A.R. Baytan, M. Russell, M.P. Chang, A. Bernard, and I.H. Maxwell 1996. Selective isolation of transiently transfected cells from a mammalian cell population with vectors expressing a membrane anchored single-chain antibody. J. Immunol. Methods. 193:17-27.
38. Krapivinsky, G., E.A. Gordon, K. Wickman, B. Velimirovic, L. Krapivinsky, and D.E. Clapham. 1995. The G-protein-gated atrial K+ channel IKACh is a heteromultimer of inwardly rectifying K(+)-channel proteins. Nature. (Lond). 374:135-141.
39. Gashler, A., and V.P. Sukhatme. 1995. Early growth response protein 1 (Egr-1): prototype of a zinc finger family of transcription factors. Prog. Nucleic Acid Res. 50:191-224.
40. Meisenholder, G.W., S.J. Martin, D.R. Green., J. Nordberg, and B.M. Babior. 1996. Events in apoptosis. Acidification is downstream of protease activation and BCL-2 protection. J. Biol. Chem. 271:16260-16262.
41. Chinnaiyan, A.M., K. O'Rourke, M. Tewari, and V.M. Dixit. 1995. FADD. a novel death domain-containing protein, interacts with the death domain of Fas and intitiates apoptosis. Cell. 81:505-512.
42. Sporn, M.B., and A.B. Robberts. 1992. Transforming growth factor-β: recent progress and new challenges. J. Cell Biol. 119:1017-1021.
43. Martikainen, P., N. Kyprianou, and J.T. Isaacs. 1990. Effect of transforming growth factor-β1 on proliferation and death of rat prostatic cells. Endocrinology. 127:2963-2968.
44. Rotello, R.J., R.C. Lieberman, A.F. Purchio, and L.E. Gerschenson. 1991. Coordinated regulation of apoptosis and cell proliferation by transforming growth factor β1 in cultured uterine epithelial cells. Proc. Natl. Acad. Sci. USA. 88:3412-3415.
45. Sekelsky, J.J., S.J. Newfeld, L.A. Raftery, E.H. Chartoff, W.M. Gelbart. 1995. Genetic characterization and cloning of mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster. Genetics. 139: 1347-1358.
46. Hoodless, P.A., T. Haerry, S. Abdollah, M. Stapleton, M.B. O'Connor, L. Attisano, and J.L. Wrana. 1996. MADRI, a MAD-related protein that functions in BMP2 signaling pathways. Cell. 85:489-500.
47. Zhang, Y., X. Feng, R. We, and R. Derynck. 1996. Receptor-associated MAD homologues synergize as effectors of the TGF-beta response. Nature (Lond). 383:168-172.
48. Centrella, M., T.L. McCarthy, and E. Canalis. 1991. Transforming growth factor-beta and remodeling of bone. J. Bone Jt. Surg. Am. Vol. 73:1418-1428.
49. INK4B through an Sp1 consensus site. J. Biol. Chem. 270: 26750-26753.
50. Alexandrow, M.G., and H.L. Moses. 1995. Transforming growth factor β1 and cell cycle regulation. Cancer Res. 55:1452-1457.
51. Datto, M.B., Y. Yu, and X.F. Wang. 1995. Functional analysis of the transforming growth factor beta responsive elements in the WAF1/Cip1/p21 promoter. J. Biol. Chem. 270:28623-28628.
52. McConkey, D.J., and S. Orrenius. 1994. Signal transduction pathways to apoptosis. Trends Cell Biol. 4:370-374.