Purification of 5-hydroxytryptamine3 receptors from porcine brain

British Journal of Pharmacology

Volumn 122, Issue 4, 1997, Pages 655-662

  Fletcher, Stephanie ^a   Barnes, Nicholas M ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

5 HT3 receptor; 5 Hydroxytryptamine; Pig brain; Purification; Receptor subunits; Solubilization

Indexed keywords

BRAIN RECEPTOR; DETERGENT; GR 119566X; RADIOLIGAND; RECEPTOR SUBUNIT; SEROTONIN 3 RECEPTOR; TRITON X 100; UNCLASSIFIED DRUG; ZACOPRIDE; BENZAMIDE DERIVATIVE; FUSED HETEROCYCLIC RINGS; SEROTONIN ANTAGONIST; SEROTONIN RECEPTOR;

ANIMAL TISSUE; ARTICLE; BINDING SITE; BRAIN; BRAIN CORTEX; COMPETITION; DOT HYBRIDIZATION; HOMOGENATE; IMMUNOBLOTTING; LIGAND BINDING; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; RECEPTOR BINDING; RECEPTOR SOLUBILIZATION; SILVER STAINING; SWINE; AFFINITY CHROMATOGRAPHY; ANIMAL; BINDING COMPETITION; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN BINDING; RADIOASSAY; WESTERN BLOTTING;

ANIMALS; BENZAMIDES; BICYCLO COMPOUNDS, HETEROCYCLIC; BINDING, COMPETITIVE; BLOTTING, WESTERN; CEREBRAL CORTEX; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; PROTEIN BINDING; RADIOLIGAND ASSAY; RECEPTORS, SEROTONIN; RECEPTORS, SEROTONIN, 5-HT3; SEROTONIN ANTAGONISTS; SWINE;

EID: 0030609119     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0701439     Document Type: Article

References (41)

1. 3 receptors. Eur. J. Pharmacol., 296, 227-230.
2. s receptor subunit. Mol. Pharmacol., 48, 1054-1062.
3. 3 antagonists in neuropsychiatric disorders. CNS Drugs, 3, 364-392.
4. 3 receptor type binding sites purified from NG108-15 cells. J. Neurochem., 59, 1692-1701.
5. BOESS, F.G. & MARTIN, I.L. (1994). Molecular biology of 5-HT receptors. Neuropharmacology, 33, 275-317.
6. 3H]RS-42358-197: evidence for receptor heterogeneity. J. Neurochem., 61, 1927-1932.
7. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
8. BRENNAN, C. SCOTLAND, P.B., FROEHNER, S.C. & HENDERSON, L.P. (1992). Functional properties of acetylcholine receptors coexpressed with the 43K protein in heteroglogous cell systems. Develop. Biol., 149, 100-111.
9. 3 receptors in three isolated preparations derived from guinea-pig tissues. Br. J. Pharmacol., 101, 591-598.
10. CHEN, Y., FOURNIER, A., COUVINEAU, A., LABURTHE, M. & AMIRANOFF, B. (1993). Purification of a galanin receptor from pig brain. Proc. Natl. Acad. Sci. U.S.A., 90, 3845-3849.
11. COCKCROFT, V.B., OSGUTHORPE, D.J., BARNARD, E.A., FRIDAY, A.E. & LUNT, G.G. (1990). Ligand gated ion channels, homology and diversity. Mol. Neurobiol., 4, 129-169.
12. 3 receptors are membrane ion channels. Nature, 339, 706-709.
13. 3 receptor recognition site expressed in pig cerebral cortex. Br. J. Pharmacol., 116, 154P.
14. 3 receptors. Br. J. Pharmacol., 118, 30P.
15. 3 receptor in pig forebrain. Brain Res., 729, 281-284.
16. 3 receptor complex purified from pig brain. Br. J. Pharmacol., 120, 122P.
17. FROEHNER, S.C., LUETJE, C.W., SCOTLAND, P.B. & PATRICK, J. (1990). The postsynaptic 43K protein clusters muscle nicotinic acetylcholine receptors in Xenopus oocytes. Neuron, 5, 403-410.
18. GRAHAM, D., PFEIFFER, F., SIMLER, R. & BETZ, H. (1985). Purification and characterization of the glycine receptor of pig spinal cord. Biochemistry, 24, 990-994.
19. 3 receptors provides new insights into their maturation and structure. J. Biol. Chem., 270, 6056-6061.
20. 3 receptor A subunit. Eur. J. Pharmacol., 245, 187-192.
21. HOYER, D., CLARKE, D.E., FOZARD, J.R., HARTIG, P.R., MARTIN, GR., MYELCHARANE, E.J., SAXENA, P.R. & HUMPHREY, P.P.A. (1994). VII. International union of pharmacology classification of receptors for 5-hydroxytryptamine (serotonin). Pharmacol. Rev., 46, 157-203.
22. HUSSY, N., LUKAS, W. & JONES, K.A. (1994). Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors. J. Physiol., 481, 311-323.
23. ISENBERG, K.E., UKHUN, I.A., HOLSTAD, S.G., JAFRI, S., UCHIDA, U., ZORUMSKI, C.F. & YANG, J. (1993). Partial cDNA cloning and NGF regulation of a rat 5-HT3 receptor subunit. Mol. Neurosci., 5, 121-124.
24. 3 receptor antagonists, YM060, YM114 (KAE-393), granisetron and ondansetron in rat vagus nerve and cerebral cortex. Neuropharmacology, 34, 631-637.
25. 3 receptor reveals species specific sensitivity to curare antagonism. Soc. Neurosci. Abstr., 18, 249.
26. 3 receptors. Naunyn-Schmiedeberg's Arch. Pharmacol., 342, 22-30.
27. KILPATRICK, G.J. & TYERS, M.B (1992). Inter-species variants of the 5-HT3 receptor. Biochem. Soc. Trans., 20, 118-121.
28. KIRSCH, J., LANGOSCH, D., PRIOR, P., LITTAUER, U.Z., SCMITT, B. & BETZ, H. (1991). The 93-kDa glycine receptor-associated protein hinds to tubulin. J. Biol. Chem., 266, 22242-22245.
29. 3 receptors from N1E-115 neuroblastoma cells. Mol. Pharmacol., 41, 18-23.
30. 3 receptor, a serotonin-gated ion channel. Science, 254, 432-437.
31. 3 receptor from NCB20 cells. J. Biol. Chem., 265, 13572-13577.
32. 3 receptor: heterogeneity in distribution and function among species. Mol. Pharmacol., 48, 407-416.
33. MORALES, M., BATTENBERG, E., DE LECEA, L., SANNA, P.P. & BLOOM, F.E. (1996). Cellular and subcellular immunolocalization of the type 3 serotonin receptor in the rat central nervous system. Mol. Brain Res., 36, 251-260.
34. 3 receptors by functional epitope tagging. J. Neurochem., 66, 1027-1032.
35. NEUGEBAUER, J. (1992). A Guide to the Properties and Uses of Detergents in Biology and Biochemistry, printed by Calbiochem-Novabiochem Corporation.
36. 3H]-granisetron binding sites in brain and peripheral tissues of the mouse. Naunyn-Schmiedeberg's Arch. Pharmacol., 351, 221-228.
37. 3 receptors. Trends Pharmacol. Sci., 13, 391-397.
38. PHILLIPS, W.D., KOPTA, C., BLOUNT, P., GARDNER, P.D., STEINBACH, J.H. & MERLIE, J.P. (1991). ACh receptor-rich membrane domains organized in fibroblasts by recombinant 43-kilodalton protein. Science, 251, 568-570.
39. STRANGE, P.G. (1988). The structure and mechanism of neurotransmitter receptors. Implications for the structure and function of the central nervous system. Biochem. J., 249, 309-318.
40. 3-A receptor identify a 54 kDa protein affinity-purified from NCB20 cells. Mol. Neuropharmacol., 3, 167-171.
41. YU, X.M., ASKALAN, R., KEIL, G.J. & SALTER, M.W. (1997). NMDA channel regulation by channel-associated protein tyrosine kinase Src. Science, 275, 674-678.