Purification and functional characterization of PecS, a regulator of virulence-factor synthesis in Erwinia chrysanthemi

Molecular Microbiology

Volumn 20, Issue 2, 1996, Pages 391-402

  Praillet, Thierry ^a   Nasser, William ^a   Robert Baudouy, Janine ^a   Reverchon, Sylvie ^a  

^a CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; DNA BINDING; DNA SEQUENCE; ERWINIA; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DEOXYRIBONUCLEASE I; DNA FOOTPRINTING; DNA, BACTERIAL; ERWINIA; MOLECULAR SEQUENCE DATA; PECTOBACTERIUM CHRYSANTHEMI; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; VIRULENCE;

EID: 0029930138     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02626.x     Document Type: Article

References (41)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1987) Current Protocols in Molecular Biology. New York: Wiley-Interscience.
2. Aymeric, J.L., Guiseppi, A., Pascal, M.C., and Chippaux, M. (1988) Mapping and regulation of the cel genes in Erwinia chrysanthemi. Mol Gen Genet 211: 95-101.
3. Bachmann, B.S. (1983) Linkage map of Escherichia coli K12. Edition 7. Microbiol Rev 47: 180-230.
4. Bading, H. (1988) Determination of the molecular mass of DNA bound protein(s) responsible for gel electrophoresis mobility shift of linear DNA fragments examplified with purified viral myb protein. Nucl Acids Res 16: 5241-5248.
5. Barras, F., Guiseppi, A., Py, B., Creuzet, N., Chambost, J.P., and Chippaux, M. (1990) Endoglucanases of Erwinia chrysanthemi strain 3937. In Plant Pathogenic Bacteria. Klement, Z. (ed.). Budapest: Akademiai Kiado, pp. 685-694.
6. Beaulieu, C., Boccara, M., and Van Gijsegem, F. (1993) Pathogenic behavior of pectinase-defective Erwinia chrysanthemi mutants on different plants. Mol Plant-Microbe Interact 6: 197-202.
7. Bourson, C., Favey, S., Reverchon, S., and Robert-Baudouy, J. (1993) Regulation of the expression of a pelA::uidA fusion in Erwinia chrysanthemi and demonstration of synergistic action of plant extract with polygalacturonate on pectate lyase synthesis. J Gen Microbiol 139: 1-9.
8. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
9. Carey, J. (1988) Gel retardation at low pH resolves trp repressor-DNA complexes for quantitative study. Proc Nat Acad Sci USA 85: 975-979.
10. Chatterjee, A.K., Thurn, K.K., and Tyrell, D.J. (1985) Isolation and characterization of Tn5 insertion mutants of Erwinia chrysanthemi that are deficient in polygalacturonate catabolic enzymes oligogalacturonate lyase and 3-deoxy-D-glycero-2,5-hexodiulosonic deshydrogenase. J Bacteriol 162: 708-714.
11. Collmer, A., and Keen, N.T. (1986) The role of pectic enzymes in plant pathogenesis. Annu Rev Phytopath 24: 383-409.
12. Condemine, G., and Robert-Baudouy, J. (1986) Isolation of Erwinia chrysanthemi kduD mutants altered in pectin degradation. J Bacteriol 165: 937-941.
13. Condemine, G., Dorel, C., Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1992) Some of the out genes involved in the secretion of pectate lyase in Erwinia chrysanthemi are regulated by KdgR. Mol Microbiol 6: 3199-3211.
14. Expert, D., and Gill, P.R.J. (1992) Iron: a modulator in bacterial virulence and symbiotic nitrogen-fixation. In Molecular Signals in Plant-microbe Communications. Verma, D.P.S. (ed.). London: CRC Press, pp. 234-236.
15. Galas, D.J., and Schmitz, A. (1981) DNase I footprinting: a simple method for the detection of protein-DNA binding specificity. Nucl Acids Res 5: 3157-3170.
16. Hugouvieux-Cotte-Pattat, N., Reverchon, S., Condemine, G., and Robert-Baudouy, J (1986) Regulatory mutations affecting the synthesis of pectate lyases in Erwinia chrysanthemi. J Gen Microbiol 132: 2099-2106.
17. Hugouvieux-Cotte-Pattat, N., and Robert-Baudouy, J. (1987) Hexuronate catabolism in Erwinia chrysanthemi. J Bacteriol 169: 1223-1231.
18. Hugouvieux-Cotte-Pattat, N., Dominguez, H., and Robert-Baudouy, J. (1992) Environmental conditions affect the transcription of the pectinase genes of Erwinia chrysanthemi 3937. J Bacteriol 174: 7807-7818.
19. Kelemu, S., and Collmer, A. (1993) Erwinia chrysanthemi EC16 produces a second set of plant-inducible pectate lyase isozymes. Appl Environ Microbiol 59: 1759-1761.
20. Kim, J., Zwieb., Wu, C., and Adhya, S. (1989) Bending of DNA by gene-regulatory proteins: construction and use of a DNA bending vector. Gene 85: 15-23.
21. Lammli, M., Paci, M., Pon, C.L., Losso, M.A., Miano, A., Pawlik, R.T., Gianfrancesschi, G.L., and Gualerzi, C.O. (1984) Proteins from the prokaryotic nucleoid: biochemical and H-NMR studies on three bacterial histon-like proteins. Adv Exp Med Biol 179: 467-477.
22. Libbi, S.J., Goebel, W., Ludwig, A., Buchmeier N., Bowe, F., Fang, F., Guiney, D., Songer, J.G., and Heffron, F. (1994) A cytolysin encoded by Salmonella is required for survival within macrophages. Proc Natl Acad Sci USA 91: 489-493.
23. Lojkowska, E., Masclaux, C., Boccara, M., Robert-Baudouy, J., and Hugouvieux-Cotte-Pattat, N. (1995) Characterization of the pelL gene encoding a novel pectate lyase of Erwinia chrysanthemi 3937. Mol Microbiol 16: 1183-1195.
24. Lomovskaya, O., Lewis, K., and Matin, A. (1995) EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB. J Bacteriol 177: 2328-2334.
25. Martin, G.M., and Rosner, J.L. (1995) Binding of purified multiple antibiotic-resistance repressor protein MarR to mar operator sequences. Proc Natl Acad Sci USA 92: 5456-5460.
26. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
27. Nasser, W., Condemine, G., Plantier, R., Anker, D., and Robert-Baudouy, J. (1991) Inducing properties of analogs of 2-keto-3-deoxygluconate on the expression of pectinase genes of Erwinia chrysanthemi. FEMS Microbiol Lett 81: 73-78.
28. Nasser, W., Awadé, A.C., Reverchon, S., and Robert-Baudouy, J. (1993) Pectate lyase from Bacillus subtilis: molecular characterization of the gene, and properties of the cloned enzyme. FEBS Lett 335: 319-326.
29. Nasser, W., Reverchon, S., Condemine, G., and Robert-Baudouy, J. (1994) Special interactions of the Erwinia chrysanthemi KdgR repressor with different operators genes involved in pectinolysis. J Mol Biol 236: 427-440.
30. Pabo, C.O., and Sauer, R.T. (1992) Transcription factors: structural families and principle of DNA recognition. Annu Rev Biochem 61: 1053-1095.
31. Reverchon, S., Nasser, W., and Robert-Baudouy, J. (1994) pecS: a locus controlling pectinase, cellulase and blue pigment production in Erwinia chrysanthemi. Mol Microbiol 11: 1127-1139.
32. Roper, O.I., Fawcett, T., and Cooper, R.A. (1993) The Escherichia coli C homoprotechatechuate degradative operon: hpc gene order, direction of transcription and control of expression. Mol Gen Genet 237: 241-250.
33. Russel, M., and Model, P. (1984) Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid. J Bacteriol 159: 1034-1039.
34. Sakakibara, H., Watanabe, M., Hase, T., and Sugiyama, T. (1991) Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthetase in maize leaf. J Biol Chem 226: 2028-2035.
35. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
36. Spassky, A., Rimsky, S., Garreau, H, and Buc, H. (1984) H1a, an E. coli DNA binding protein which accumulates in stationary phase, strongly compacts DNA in vitro. Nucl Acids Res 12: 5321-5340.
37. Studier, F.W., Rosenberg, A.H., Dunn, J.J., and Dubendorff, J.W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Meth Enzymol 185: 60-89.
38. Tabor, S., and Richardson, C.C. (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Nat Acad Sci USA 82: 1074-1078.
39. Tippner, D., Afflerbach, H., Bradaczek, C., and Wagner, R. (1994) Evidence for a regulatory function of the histon-like Escherichia coli protein H-NS in ribosomal RNA synthesis. Mol Microbiol 11: 589-604.
40. Ussery, D.W., Hinton, J.C.D., Jordi, B.J.A.M., Granum, P.E., Seirafi, A., Stephen, R.J., Tupper, A.E., Berridge G., Sidebothan, J.M., and Higgins, C.F. (1994) The chromatin-associated protein H-NS. Biochimie 76: 968-980.
41. Wang, Q., and Calvo J.M. (1993) Lrp, a major regulatory protein in Escherichia coli, bends DNA and can organize the assembly of a higher-order nucleoprotein structure. EMBO J 12: 2495-2501.