Novel steroids from Trichilia hirta as identified by nanoprobe INADEQUATE 2D-NMR spectroscopy

Tetrahedron Letters

Volumn 37, Issue 44, 1996, Pages 7875-7878

  Chauret, Denise C ^a   Durst, Tony ^a   Arnason, John T ^a   Sanchez Vindas, Pablo ^b   San Roman, Lorena ^b   Poveda, Luis ^b   Keifer, Paul A ^c  

^a UNIVERSITY OF OTTAWA   (Canada)

^b UNIVERSIDAD NACIONAL   (Costa Rica)

^c Varian Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 HYDROXYANDROSTA 1,4 DIENE 3,16 DIONE; 3 HYDROXYPREGNANE 2,16 DIONE; ANDROSTANE DERIVATIVE; PREGNANE DERIVATIVE; STEROID; UNCLASSIFIED DRUG;

ARTICLE; DRUG IDENTIFICATION; DRUG ISOLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NUCLEAR MAGNETIC RESONANCE; PHYTOCHEMISTRY; PLANT;

TRICHILIA HIRTA;

EID: 0030605099     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(96)01803-5     Document Type: Article

References (21)

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16. 1H Nanoprobe were used to determine the structures of microgram quantities of novel carbohydrates isolated from tissues (Manzi, A.; Salimath, P. V.; Spiro, R. C.; Keifer, P. A.; Freeze, H. H. J. Biol. Chem. 1995, 270, 9154-9163).
17. 1H Nanoprobe were used to determine the structures of microgram quantities of novel carbohydrates isolated from tissues (Manzi, A.; Salimath, P. V.; Spiro, R. C.; Keifer, P. A.; Freeze, H. H. J. Biol. Chem. 1995, 270, 9154-9163).
18. 1 was 6.2 sec for C-16. The repetition rate was 9.0 sec (including a 136 msec acquisition time) for 128 scans for each of 92 × 2 increments (States-TPPI). Apodizations and zero-filling do not influence the FRED analysis (output shown in Fig. 1) and were not used. The C3-C4 bond was presumably below the detection limit, while the near-coincidental C-10 and C-13 resonances caused methyl-group assignments to be ambiguous.
19. 3) d 217.19 (C-16), 181.46 (C-3), 172.06 (C-5), 146.23 (C-2), 123.66 (C-1), 121.30 (C-4), 55.46 (C-17), 53.38 (C-8), 50.68 (C-14), 44.04 (C-10*), 39.29 (C-13*), 39.25 (C-15), 37.76 (C-11), 34.84 (C-9), 34.05 (C-7), 32.62 (C-6), 22.84 (C-12), 19.74 (C-19), and 18.09 (C-18), *Assignment tentative.
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