Chemical composition of the coccolithophorid Emiliania huxleyi under light-limited steady state growth

Journal of Experimental Marine Biology and Ecology

Volumn 207, Issue 1-2, 1996, Pages 149-160

  Fernández, Emilio ^a   Fritz, Jennifer J ^b   Balch, William M ^c  

^a UNIVERSITY OF VIGO   (Spain)

^b UNIVERSITY OF MIAMI   (United States)

^c BIGELOW LABORATORY FOR OCEAN SCIENCES   (United States)

Author keywords

Biochemical composition; Carbon partitioning; Coccolithophorid; Emiliania huxleyi

Indexed keywords

CARBOHYDRATE; CARBON; CHLOROPHYLL A; LIPID; PROTEIN;

ALGA; ARTICLE; CARBOHYDRATE ANALYSIS; CELL LEVEL; CHEMICAL COMPOSITION; CONTINUOUS CULTURE; FLUOROMETRY; GROWTH RATE; LIPID COMPOSITION; NONHUMAN; PHOTOSYNTHESIS; PROTEIN ANALYSIS; TOTAL ORGANIC CARBON;

EMILIANIA HUXLEYI;

EID: 0030589535     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-0981(96)02657-3     Document Type: Article

References (48)

1. Balch, W.M., Holligan, P.M., Ackleson, S.G. and Voss, K.J., 1991. Biological and optical properties of mesoscale coccolithophore blooms in the Gulf of Maine. Limnol. Oceanogr., Vol. 36, pp. 629-643.
2. Balch, W.M., Holligan, P.M. and Kilpatrick, K.A., 1992. Calcification, photosynthesis and growth of the bloom-forming coccolithophore, Emiliania huxleyi. Cont. Shelf Res., Vol. 12, pp. 1353-1374.
3. Balch, W.M., Kilpatrick, K.A., Holligan, P.M. and Cucci, T., 1993. Coccolith production and detachment by Emiliania huxleyi (Prymnesiophyceae). J. Phycol., Vol. 29, pp. 566-575.
4. Bratbak, G., Egge, J.K. and Heldal, M., 1993. Viral mortality of the marine alga Emiliania huxleyi (Haptophyceae) and termination of algal blooms. Mar. Ecol. Prog. Ser., Vol. 93. pp. 39-48.
5. Bratbak, G., Levasseur, M., Michaud, S., Cantin, G., Fernandez, E., Heimdal, B.R. and Heldal, M., 1995. Viral activity in relation to Emiliania huxleyi blooms: a mechanism of DMSP released? Mar. Ecol. Prog. Ser., Vol. 128a, pp. 133-142.
6. Chan, A.T., 1978. Comparative physiological study of marine diatoms and dinoflagellates in relation to irradiance and cell size. I. Growth under continuous light. J. Phycol., Vol. 14, pp. 396-402.
7. Claustre, H. and Gostan, J., 1987. Adaptation of biochemical composition and cell size to irradiance in two microalgae: possible ecological implications. Mar. Ecol Prog. Ser., Vol. 40, pp. 167-174.
8. Embadi, D. and Berland, B., 1989. Variation in lipid class composition during batch growth of Nannochloropsis salina and Pavlova lutheri. Mar. Chem., Vol. 26, pp. 215-225.
9. Fernández, E., Boyd, P., Holligan, P.M. and Harbour, D.S., 1993. Production of organic and inorganic carbon within a large scale coccolithophore bloom in the North Atlantic ocean. Mar. Ecol. Prog. Ser., Vol. 97, pp. 271-285.
10. Fernández, E., Balch, W.M., Mararion, E. and Holligan, P.M., 1994a. High rates of lipid biosynthesis in cultured, mesocosm and coastal populations of the coccolithophore Emiliania huxleyi. Mar. Ecol. Prog. Ser., Vol. 114a, pp. 13-22.
11. Fernández, E., Maranón, E., Harbour, D.S. and Pingree, R.D., 1994b. Phytoplankton carbon incorporation patterns and biochemical composition of paniculate matter in the eastern north-Atlantic subtropical region. J. Plankton Res., Vol. 16, pp. 1627-1644.
12. Fernández, E., Maranón, E. and Balch, W.M., 1996a. Intracellular carbon partitioning in the coccolithophorid Emiliania huxleyi. J. Mar. Sys., (in press).
13. Fernández, E., Maranón, E., Harbour, D.S., Kristiansen, S. and Heimdal, B.R. 1996b. Patterns of carbon and nitrogen uptake during blooms of Emiliania huxleyi in two Norwegian fjords. J. Plankton Res., (in press).
14. Fernández, E., Serret, P., Madariaga, I., Harbour, D.S. and Davies, A.G., 1992. Photosynthetic carbon metabolism and biochemical composition of spring phytoplankton assemblages enclosed in microcosms: the diatom-Phaeocystis sp. succession. Mar. Ecol. Prog. Ser., Vol. 90, pp. 89-102.
15. Fritz, J.J. and Balch, W.M. A light-limited continuous culture study of Emiliania huxleyi: determination of coccolith detachment and its relevance to cell sinking. J. Exp. Mar. Biol. Ecol., Vol. 207, pp. 127-147.
16. Geider, R.J., Platt, T. and Raven, R.A., 1986. Size dependence of growth and photosynthesis in diatoms. Mar. Ecol. Prog. Ser., Vol. 30, pp. 93-104.
17. Harris, R.P., 1994,. Zooplankton grazing on the coccolithophore Emiliania huxleyi and its role in inorganic carbon flux. Mar. Biol., Vol. 119, pp. 431-439.
18. Holligan, P.M., Fernández, E., Aiken, J., Balch, W.M., Boyd, P., Burkill, P.H., Finch, M., Groom, S.B., Malin, G., Muller, K., Purdie, D.A., Robinson, C., Trees, C., Turner, S.M. and van der Wal. P.A., 1993. A biogeochemical study of the coccolithophore Emiliania huxleyi in the North Atlantic. Global Biogeochem. Cycles, Vol. 7, pp. 879-900.
19. Holligan, P.M., Viollier, M., Harbour, D.S., Camus, P. and Champagne-Philippe, M., 1983. Satellite and ship studies of coccolithophore production along a continental shelf-edge. Nature, Vol. 304, pp. 339-342.
20. Keller, M.D., Selvin, R.C., Claus, W. and Guillard, R.R.L., 1987. Media for the culture of oceanic ultraphytoplankton. J. Phycol., Vol. 23, pp. 633-638.
21. Klaveness, D. and Paasche, E., 1970. Physiology of coccolithophorids. In, Biochemistry and Physiology of Protozoa, Vol. 1, edited by Levandowski, M. and Hutner, S.H., Academic Press, London, pp. 191-213.
22. Konopka, A., 1983. The effect of nutrient limitation and its interaction with light upon the products of photosynthesis in Merismopedia tenuissima (Cyanophyceae). J. Phycol., Vol. 19, pp. 403-409.
23. Laws, H.A., 1991. Photosynthetic quotients, new production and net community production in the open ocean. Deep-Sea Res., 38(I): 143-167.
24. Laws, E.A. and Wong, D.C.L., 1978. Studies of carbon and nitrogen metabolism by three marine phytoplankton species in nitrate-limited continuous culture. J. Phycol., Vol. 14, pp. 406-416.
25. Mague, T.H., Friberg, E., Hughes, D.J. and Morris, I., 1980. Extracellular release of carbon by marine phytoplankton: a physiological approach. Limnol. Oceanogr., Vol. 25, pp. 262-279.
26. Malin, G., Turner, S., Liss, P., Holligan, P.M. and Harbour, D.S., 1993. Dimethylsulphoniopropionate in the north east Atlantic during the summer coccolithophore bloom. Deep-Sea Res., Vol. 40, pp. 1487-1508.
27. Maranón, E., Fernández, E., Harris, R.P. and Harbour, D.S., 1996. Effects of the diatom-Emiliania huxleyi succession on photosynthesis, calcification and carbon metabolism by size-fractionated phytoplankton. Hydrobiologia., 317: 189-199.
28. Morris, I., 1981. Photosynthetic products, physiological state and phytoplankton growth. Can. Bull. Fish. Aquat. Sci., Vol. 210, pp. 83-102.
29. Nimer, N.A., Brownlee, C. and Merret, M.J., 1994. Carbon dioxide availability, intracellular pH and growth rate of the coccolithophore Emiliania huxleyi. Mar. Ecol. Prog. Ser., Vol. 109, pp. 257-262.
30. Nimer, N.A. and Merret, M.J., 1993. Calcification rate in Emiliania huxleyi Lohman in response to light, nitrate and availability of inorganic carbon. New Phytol., Vol. 123, pp. 673-677.
31. Paasche, E., 1968a. Biology and physiology of Coccolithophorids. Ann. Rev. Microbiol., Vol. 22, pp 71-86.
32. Paasche, E., 1968b. The effect of temperature, light intensity, and photoperiod on coccolith formation. Limnol. Oceanogr., Vol. 13, pp. 178-181.
33. Paasche, E. and Brubak, S., 1994. Enhanced calcification in the coccolithophorid Emiliania huxleyi under phosphorus limitation. Phycologia., 33(5): 324-330.
34. Paasche, E. and Klaveness, D., 1970. A physiological comparison of coccolith-forming and naked cells of Coccolithus huxleyi. Arch. Mikrobiol., Vol. 73, pp. 143-152.
35. Pond, D.W. and Harris, R.P. The lipid composition of the coccolithophore Emiliania huxleyi and its possible ecophysiological significance. J. Mar. Biol. Ass. U.K., (submitted).
36. Redalje, D.G. and Laws, E.A., 1983. The effects of environmental factors on growth and the biochemical composition of marine diatoms. I. Light and temperature effects. J. Exp. Mar. Biol. Ecol., Vol. 68, pp. 59-79.
37. Rivkin, R.B., 1989. Influence of irradiance and spectral quality on the carbon metabolism of phytoplankton. I. Photosynthesis, chemical composition and growth. Mar. Ecol. Prog. Ser., Vol. 55, pp. 291-304.
38. Schofield, O., Bidigare, R.R. and Prézelin, B.B., 1990. Spectral photosynthesis, quantum yield and blue-green light enhancement of productivity rates in the diatom Chaetoceros gracile and the prymnesiophyte Emiliania huxleyi. Mar. Ecol. Prog. Ser., Vol. 64, pp. 175-186.
39. Sharp, J.H., Underhill, P.A. and Frake, A.C., 1980. Carbon budgets in batch and continuous cultures: how can we understand natural physiology of manne phytoplankton? J. Plankton Res., Vol. 2, pp. 213-222.
40. Shifrin, N.S. and Chisholm, S.W., 1981. Phytoplankton lipids: interspecific differences and effects of nitrate. silicate and light-dark cycles. J. Phcol. 17: 374-384.
41. Sikes, C.S., Roer, R.D. and Wilbur, K.M., 1980. Photosynthesis and coccolith formation: Inorganic carbon sources and net inorganic reaction of deposition. Limnol. Oceanogr., Vol. 25, pp. 248-261.
42. Sekino, K. and Shiraiwa, Y., 1994. Accumulation and utilization of dissolved inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi. Plant Cell Physiol. Vol. 35, pp. 353-361.
43. Smith, R.E.H. and Geider, R.J., 1985. Kinetics of intracellular carbon allocation in a marine diatom. J. Exp. Mar. Biol. Ecol., Vol. 93, pp. 191-210.
44. Terry, K.L., Hirata, J. and Laws, E.A., 1983. Light-limited growth of two strains of the manne diatom Phaeodactylum tricornutum Bohlin: Chemical composition, carbon partitioning and the diel peridicity of physiological processes. J. Exp. Mar. Biol. Ecol., Vol. 68, pp. 209-227.
45. Thompson, P.A., Guo, M. and Harrison, P.J., 1993. The influence of irradiance on the biochemical composition of three phytoplankton species and their nutritional value for larvae of the Pacific Oyster (Crassostrea gigas). Mar. Biol., Vol. 117, pp. 259-268.
46. Thompson, P.A., Harrison, P.J. and Parslow, J.S., 1991. Influence of irradiance on cell volume and carbon quota for ten species of marine phytoplankton. J. Pycol., Vol. 27, pp. 351-360.
47. Van Bleijswijk, J.D.L., Kempers, R.S., Veldhuis, M.J. and Westbroek, P., 1994. Cell and growth characteristics of types A and B of Emiliania huxleyi (Prymnesiophyceae) as determined by flow cytometry and chemical analyses. J. Phycol., Vol. 30, pp. 230-241.
48. Volkman, J.K., Eglinton, G., Corner, E.D.S. and Forsberg, T.E.V, 1980. Long-chain alkenes and alkenones in the marine coccolithophorid Emiliania huxleyi. Phytochemistry, Vol. 19, pp. 2619-2622.