A light-limited continuous culture study of Emiliania huxleyi: Determination of coccolith detachment and its relevance to cell sinking

Journal of Experimental Marine Biology and Ecology

Volumn 207, Issue 1-2, 1996, Pages 127-147

  Fritz, Jennifer J ^a   Balch, William M ^b  

^a UNIVERSITY OF MIAMI   (United States)

^b BIGELOW LABORATORY FOR OCEAN SCIENCES   (United States)

Author keywords

Coccolith detachment rates; Continuous culture; Emiliania huxleyi; Growth rates; Sinking rates

Indexed keywords

CALCIUM CARBONATE; CARBON; CHLOROPHYLL; NITROGEN;

ALGA; ALKALINITY; ARTICLE; CELL DENSITY; CELL GROWTH; CONTINUOUS CULTURE; CULTURE MEDIUM; FLUORESCENCE MICROSCOPY; GROWTH RATE; NONHUMAN; PH; SPECIES; TOTAL ORGANIC CARBON;

EMILIANIA HUXLEYI;

COCCOLITH; COCCOLITHOPHORID; COCCOLITHS; DETACHMENT; GROWTH; SINKING; SINKING RATE;

EID: 0030589205     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-0981(96)02633-0     Document Type: Article

References (40)

1. Ackleson, S., W.M. Balch and P.M. Holligan, 1988. White waters of the Gulf of Maine. Oceanography, Vol. 1, pp. 18-22.
2. Balch, W.M., J.J. Fritz, and E. Fernandez, 1996a. Decoupling of calcification and photosynthesis in the coccolithophore Emiliania huxleyi under steady state growth. Mar. Ecol. Prog. Ser., in press
3. Balch, W.M., P.M. Holligan, S.G. Ackleson and K.J. Voss, 1991. Biological and optical properties of mesoscale coccolithophore blooms in the Gulf of Maine. Limnol. Oceanogr., Vol. 36, pp. 629-643.
4. Balch, W.M., P.M. Holligan and K.A. Kilpatrick, 1992. Calcification, photosynthesis and growth of the bloom-forming coccolithophore Emiliania huxleyi. Cont. Shelf. Res., Vol. 12, pp. 1353-1374.
5. Balch, W.M., K.A. Kilpatrick, P.M. Holligan and T. Cucci, 1993. Coccolith production and detachment by Emiliania huxleyi (Prymnesiophyceae). J. Phycol., Vol. 29, pp. 566-575.
6. Balch, W.M., K.A. Kilpatrick, P.M. Holligan, D. Harbour and E. Fernandez, 1996b. The 1991 coccolithophore bloom in the Central North Atlantic. II. Relating optics to coccolith concentration. Limnol. Oceanog., in press.
7. Brand, L.E., 1981. Genetic variability in reproduction rates in marine phytoplankton populations. Evolution, Vol. 35(6), pp. 1117-1127.
8. Cullen, J.J. and S.G. Horrigan, 1981. Effects of nitrate on the diurnal vertical migration, carbon to nitrogen ratio, and the photosynthetic capacity of the dinoflagellate Gymnodinium splendens. Mar. Biol., Vol. 62, pp. 81-89.
9. Eppley, R.W. and D.L. Dyer, 1965. Predicting production in light-limited continuous cultures of algae. Appl. Microbiol., Vol. 13, pp. 833-837.
10. Eppley, R.W., R.W. Holmes and J.D.H. Strickland, 1967. Sinking rates of marine phytoplankton measured with a fluorometer. J. Exp. Mar. Biol. Ecol., Vol. 1, pp. 191-208.
11. Eppley, R.W. and E.H. Renger, 1974. Nitrogen assimilation of an oceanic diatom in nitrogen-limited continuous culture. J. Phycol., Vol. 10, pp. 15-23.
12. Eppley, R.W., J.N. Rogers, J.J. McCarthy and A. Sournia, 1971. Light/dark periodicity in nitrogen assimilation of the marine phytoplankters Skeletonema costatum and Coccolithus huxleyi in N-limited chemostat culture. J. Phycol., Vol. 7, pp. 150-154.
13. Fernandez, E., W.M. Balch, E. Maranõn and P.M. Holligan, 1994. High rates of lipid biosynthesis in cultured, mesocosm and coastal populations of the coccolithophore Emiliania huxleyi. Mar. Ecol. Prog. Ser., Vol. 114, pp 13-22.
14. Fernández, E., P. Boyd, P.M. Holligan and D.S. Harbour, 1993. Production of organic and inorganic carbon within a large-scale coccolithophore bloom in the northeast Atlantic Ocean. Mar. Ecol. Prog. Ser., Vol. 97, pp. 271-285.
15. Fernández, E., J.J. Fritz and W.M. Balch, 1996. Chemical composition of the coccolithophorid Emiliania huxleyi under steady state growth. J. Exp. Mar. Biol. Ecol., Vol. 207, pp. 149-160.
16. Holligan, P.M., E. Fernández, J. Aiken, W.M. Balch, P Boyd, P.H. Burkill, M. Finch, S.B. Groom, G. Malin, K, Muller, D.A. Purdie, C. Robinson, C.C. Trees, S.M. Turner and P. van der Wal, 1993. A biogeochemical study of the coccolithophore Emiliania huxleyi in the North Atlantic. Global Biogeochemical Cycles, Vol. 7, pp. 879-900.
17. Holligan, P.M., M. Viollier, D.S. Harbour, P. Camus and M. Champagne-Philippe, 1983. Satellite and ship studies of coccolithophore production along a continental shelf edge. Nature, Vol. 304, pp. 339-342.
18. Holm-Hansen, O., C.J. Lorenzen, R.W. Holmes and J.D.H. Strickland, 1965. Fluorometric determination of chlorophyll. J. Cons. Perm. Int. Explor. Mer., Vol. 30, pp. 3-15.
19. Honjo, S., 1976. Coccoliths: Production, transportation and sedimentation. Mar Micropaleont, Vol. 1, pp. 65-79.
20. Keller, M.D., R.C. Selvin, W. Claus and R.R.L. Guillard, 1987. Media for the culture of oceanic ultraphytoplankton. J.Phycol., Vol. 23, pp. 633-638.
21. Laws, E.A. and T.T. Bannister, 1980. Nutrient-and light-limited growth of Thalassiosira fluviatilis in continuous culture, with implications for phytoplankton growth in the ocean. Limnol. Oceanog., Vol. 25, pp. 457-473.
22. Meyer, S.L., 1975. Data Analysis for Scientists and Engineers. John Wiley and Sons, Inc. New York.
23. Monod, J., 1950. La technique de culture continue: theorie et applications. Ann. Inst. Pasteur, Paris, Vol. 79, pp. 390-410.
24. Morel, A. and A. Bricaud, 1981. Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton. Deep Sea Res., Vol. 28A, pp. 1375-1393.
25. Munk, W.H. and G.A. Riley, 1952. Absorption of nutrients by aquatic plants. J. Mar. Res., Vol. 11, pp. 215-240.
26. Nimer, N.A. and M.J. Merrett, 1993. Calcification rate in Emiliania huxleyi Lohmann in response to light, nitrate and availability of inorganic carbon. New Phytol., Vol. 123, pp. 673-677.
27. Novick, A. and S. Szilard, 1950. Description of the chemostat. Science, Vol. 112, pp. 715-716.
28. Paasche, E., 1962. Coccolith formation. Nature, Vol. 193, pp. 1094-1095.
29. Paasche, E., 1964. A tracer study of the inorganic carbon uptake during coccolith formation and photosynthesis in the coccolithophorid Coccolithus huxleyi. Physiol. Plant., Supp. III.
30. Paasche, E., 1968. Biology and physiology of coccolithophorids. Ann. Rev. Microbiol., Vol. 22, pp. 71-86.
31. Paasche, E. and S. Brubak, 1994. Enhanced calcification in the coccolithophorid Emiliania huxleyi (Haptophyceae) under phosphorus limitation. Phycologia, Vol. 33, pp. 324-330.
32. Paasche, E. and D. Klaveness, 1970. A physiological comparison of coccolith-forming and naked cells of Coccolithus huxleyi. Arch. Mikrobiol., Vol 73, pp. 143-152.
33. Parsons, T.R., Y. Maita and C.M. Lalli, 1984. A Manual of Chemical and Biological Methods for Seawater Analysis. Pergamon Press, New York.
34. Rhee, G.Y., 1978. Effects of N/P atomic ratios and nitrate limitation on algal growth, cell composition, and nitrate uptake: a study of dual nutrient limitation. Limnol. Oceanogr., Vol. 23, pp. 10-25.
35. Rhee, G.Y., 1980. Continuous culture in phytoplankton ecology. In. Advances in Aquatic Microbiology, edited by M.R. Droop and H.W. Jannasch, Volume 2. Academic Press, London, pp. 151-203.
36. Richardson. K., J. Beardall and J.A. Raven, 1983. Adaptation of unicellular algae to irradiance: an analysis of strategies. New Phytol., Vol. 93, pp. 157-191.
37. Sokal, R.R. and F.J. Rohlf, 1981. Biometry. 2nd edition. W.H. Freeman and Co., New York.
38. Westbroek, P., C.W. Brown, J. van Bleijswijk, C. Brownlee. G.J. Brummer, M. Conte, J. Egge, E. Fernández, R. Jordan, M. Knappertsbusch, J. Stefels, M. Veldhuis, P van der Wal and J. Young, 1993. A model system approach to biological climate forcing. The example of Emiliania huxleyi. Global and Planetary Change, Vol. 8, pp. 27-46.
39. Westbroek, P., E.W. de Jong, P van der Wal, A.H. Borman, J.P.M. de Vrind, D. Kok, W.C. de Bruijn and S.B. Parker, 1984. Mechanism of calcification in the marine alga Emiliania huxleyi. Phil. Trans. R. Soc. Lond., Vol. B304, pp. 435-444.
40. Yentsch C.S. and D.W. Menzel, 1963. A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence. Deep Sea Res., Vol. 10, pp. 221-231.