Proteolytic processing of human lactase-phlorizin hydrolase is a two- step event: Identification of the cleavage sites

Archives of Biochemistry and Biophysics

Volumn 336, Issue 1, 1996, Pages 27-34

  Wüthrich, Marcel ^a   Grünberg, Jürgen ^a   Hahn, Dagmar ^a   Jacob, Ralf ^b   Radebach, Ilona ^b   Naim, Hassan Y ^b   Sterchi, Erwin E ^a  

^a UNIVERSITY OF BERN   (Switzerland)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Caco 2; COS; lactase phlorizin hydrolase; MDCK; microvillus membrane; proteolytic processing; trypsin

Indexed keywords

GLYCOSYLCERAMIDASE; LACTASE; MUTANT PROTEIN; PROTEIN PRECURSOR; TRYPSIN;

ARTICLE; ENZYME SYNTHESIS; HUMAN; INTESTINE BRUSH BORDER; MICROVILLUS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROCESSING;

EID: 0030459575     PISSN: 00039861     EISSN: None     Source Type: Journal    
DOI: 10.1006/abbi.1996.0528     Document Type: Article

References (33)

1. 1. Mantei, N., Villa, M., Enzler, T., et al. (1988) EMBO J. 7, 2705-2713.
2. 2. Skovbjerg, H., Sjostrom, H., and Noren, O. (1981) Eur. J. Biochem. 114, 653-661.
3. 3. Danielsen, E. M., Skovbjerg, H., Noren, O., and Sjostrom, H. (1984) Biochem. Biophys. Res. Commun. 122, 82-90.
4. 4. Hauri, H. P., Sterchi, E. E., Bienz, D., Fransen, J. A., and Marxer, A. (1985) J. Cell. Biol. 101, 838-851.
5. 5. Naim, H. Y., Sterchi, E. E., and Lentze, M. J. (1987) Biochem. J. 241, 427-434.
6. 6. Naim, H. Y. (1992) Biochem. J. 285, 13-16.
7. 7. Lottaz, D., Oberholzer, T., Bähler, P., Semenza, G., and Sterchi, E. E. (1992) FEBS Lett. 313, 270-276.
8. 8. Buller, H. A., Montgomery, R. K., Sasak, W. V., and Grand, R. J. (1987) J. Biol. Chem. 262, 17206-17211.
9. 9. Quan, R., Santiago, N. A., Tsuboi, K. K., and Gray, G. M. (1990) J. Biol. Chem. 265, 15882-15888.
10. 10. Yeh, K. Y., Yeh, M., Pan, P. C., and Holt, P. R. (1991) Gastroenterology 101, 312-318.
11. 11. Naim, H. Y., Lacey, S. W., Sambrook, J. F., and Gething, M. J. (1991) J. Biol. Chem. 266, 12313-12320.
12. 12. Smeekens, S. P. (1993) Biotechnology 11, 182-186.
13. 13. Barr, P. J. (1991) Cell 66, 1-3.
14. 14. Schalken, J. A., Roebroek, A. J. M., Oomen, P. P. C. A., et al. (1987) J. Clin. Invest. 80, 1545-1549.
15. 15. Kiefer, M. C., Tucker, J. E., Joh, R., Landsberg, K. E., Saltman, D., and Barr, P. J. (1991) DNA Cell Biol. 10, 757-769.
16. 16. Smeekens, S. P., and Steiner, D. F. (1990) J. Biol. Chem. 265, 2997-3000.
17. 17. Smeekens, S. P., Avruch, A. S., LaMendola, J., Chan, S. J., and Steiner, D. F. (1991) Proc. Natl. Acad. Sci. USA 88, 340-344.
18. 18. Seidah, N. G., Gaspar, L., Mion, P., Marcinkiewicz, M., Mbikay, M., and Chretien, M. (1990) DNA Cell Biol. 9, 415-424.
19. 19. Creemers, J. W. M., Roebroek, A. J. M., and Van de Ven, W. J. M. (1992) FEBS Lett. 300, 82-88.
20. 20. Nakayama, K., Kim, W. S., Torii, S., et al. (1992) J. Biol. Chem. 267, 5897-5900.
21. 21. Seidah, N. G., Day, R., Hamelin, J., Gaspar, A. M., Collard, M. W., and Chretien, M. (1992) Mol. Endocrinol. 6, 1559-1570.
22. 22. Torii, S., Yamagishi, T., Murakami, K., and Nakayama, K. (1993) FEBS Lett. 316, 12-16.
23. 23. Nakagawa, T., Murakami, K., and Nakayama, K. (1993) FEBS Lett. 327, 165-171.
24. 24. Keller, P., Zecca, L., Boukamel, R., Zwicker, E., Gloor, S., and Semenza, G. (1995) J. Biol. Chem. 270, 25722-25728.
25. 25. Wüthrich, M., Creemers, J., Van de Ven, W., and Sterchi, E. (1996) Biochim. Biophys. Acta 1311, 199-203.
26. 26. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. USA 82, 488-492.
27. 27. Kunkel, T. A., Roberts, J. D., and Zakour, R. A. (1987) Methods Enzymol. 154, 367-382.
28. 28. Grünberg, J., Luginbuehl, U., and Sterchi, E. E. (1992) FEBS Lett. 314, 270-276.
29. 29. Cullen, B. R. (1987) Methods Enzymol. 152, 684-704.
30. 30. Schmidt, C., Grünberg, J., and Kruppa, J. (1992) J. Virol. 66, 2792-2797.
31. 31. Laemmli, U. K. (1970) Nature 227, 680-685.
32. 32. Naim, H. Y., Jacob, R., Naim, H., Sambrook, J. F., and Gething, M. J. (1994) J. Biol. Chem. 269, 26933-26943.
33. 33. Jacob, R., Radebach, I., Wüthrich., M., Grünberg, J., Sterchi, E., and Naim, H. (1996) Eur. J. Biochem. 236, 789-795.