Autoclave antigen retrieval technique for immunohistochemical staining of androgen receptor in formalin-fixed paraffin sections of human prostate

Acta Histochemica et Cytochemica

Volumn 29, Issue 4, 1996, Pages 311-318

  Ehara, Hidetoshi ^a   Deguchi, Takashi ^a   Koji, Takehiko ^b   Yang, Muzheng ^a   Ito, Shin Ichi ^a   Kawada, Yukimichi ^a   Nakane, Paul K ^b  

^a GIFU UNIVERSITY   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

Author keywords

Androgen receptor; Autoclave antigen retrieval; Immunohistochemistry; Prostate; Steroid hormone receptor

Indexed keywords

EID: 0030439289     PISSN: 00445991     EISSN: None     Source Type: Journal    
DOI: 10.1267/ahc.29.311     Document Type: Article

References (23)

1. van Aubel, O., Bolt-deVries, J., Blankenstein, M. A. and Schroder, F. H.: Prediction of time to progression after orchiectomy by the nuclear androgen receptor content from multiple biopsy specimens in patients with advanced prostate cancer. Prostate 12; 191-198, 1988.
2. Bankfalvi, A., Navabi, H., Bier, B., Bocker, W., Jasani, B. and Schmid, K. W.: Wet autoclave pretreatment for antigen retrieval in diagnostic immunohistochemistry. J. Pathol. 174; 223-228, 1994.
3. Barrack, E. R., Brendler, C. B. and Walsh, P. C.: Steroid receptor and biochemical profiles in prostatic cancer: Correlation with response to hormonal treatment. Prog. Clin. Biol. Res. 243A; 79-97, 1987.
4. Deguchi, T., Doi, T., Ehara, H., Ito, S., Takahashi, Y., Nishino, Y., Fujihiro, S., Kawamura, T., Komeda, H., Horie, M., Kaji, H., Shimokawa, K., Tanaka, T. and Kawada, Y.: Detection of micrometastatic prostate cancer cells in lymph nodes by reverse transcriptase-polymerase chain reaction. Cancer Res. 53; 5350-5354, 1993.
5. Ehara, H., Koji, T., Deguchi, T., Yoshii, A., Nakano, M., Nakane, P. K. and Kawada, Y.: Expression of estrogen receptor in diseased human prostate assessed by nonradioactive in situ hybridization and immunohistochemistry. Prostate 27; 304-313, 1995.
6. Horoszewicz, J. S., Leong, S. S., Ming, C. T., Wajsman, Z. L., Friedman, M., Papsidero, L., Kim, U., Chai, L. S., Kakati, S., Arya, S. K. and Sandberg, A. A.: The LNCaP cell line - A new model for studies on human prostatic carcinoma. Prog. Clin. Biol. Res. 37; 115-132, 1980.
7. Janssen, P. J., Brinkmann, A. O., Boersma, W. J. and van der Kwast, T. H.: Immunohistochemical detection of the androgen receptor with monoclonal antibody F39.4 in routinely processed, paraffin-embedded human tissues after microwave pre-treatment. J. Histochem. Cytochem. 42; 1169-1175, 1994.
8. Koji, T. and Nakane, P. K.: Recent advances in molecular histochemical techniques: In situ hybridization and southwestern histochemistry. J. Electron. microsc. 45; 119-127, 1996.
9. Kwok, S.: Procedures to minimize PCR product carry-over. In "PCR Protocol: A Guide to Methods and Applications," ed. by M. A. Innis, D. H. Gelfand, J. J. Sminsky and T. J. White, Academic Press, San Diego, 1990, pp. 142-145.
10. Login, G. R. and Dvorak, A. M.: Microwave fixation provides excellent preservation of tissue, cells and antigens for light and electron microscopy. Histochem. J. 20; 373-387, 1988.
11. Masai, M., Sumiya, H., Akimoto, S., Yatani, R., Chang, C., Liao, S. and Shimazaki, J.: Immunohistochemical study of androgen receptor in benign hyperplastic and cancerous human prostate. Prostate 17; 293-300, 1990.
12. Mayers, C. P.: Histological fixation by microwave heating. J. Clin. Pathol. 23; 273-275, 1970.
13. Morgan, J. M., Navabi, H., Schmid, K. W. and Jasani, B.: Possible role of tissue-bound calcium ions in citrate-mediated high-temperature antigen retrieval. J. Pathol. 174; 301-307, 1994.
14. Norton, A. J., Jordan, S. and Yeomans, P.: Brief, high-temperature heat denaturation (pressure cooking): a simple and effective method of antigen retrieval for routinely processed tissues. J. Pathol. 173; 371-379, 1994.
15. Sadi, M. V. and Barrack, E. R.: Image analysis of androgen receptor immunostaining in metastatic prostate cancer. Cancer 71; 2574-2580, 1993.
16. Shi, S-R., Key, M. E. and Kalra, K. L.: Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J. Histochem. Cytochem. 39; 741-748, 1991.
17. Shi, S-R., Chaiwun, B., Young, L., Cote, R. J. and Taylor, C. R.: Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections. J. Histochem. Cytochem. 41; 1599-1604, 1993.
18. Shin, R. W., Iwaki, T., Kitamoto, T. and Tateishi, J.: Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues. Methods Lav. Invest. 64; 693-702, 1991.
19. Slayden, O. D., Koji, T. and Brenner, R. M.: Microwave-stabilization enhances immunocytochemical detection of estrogen receptor in frozen sections of macaque oviduct. Endocrinology 136; 4012-4021, 1995.
20. Takeda, H., Chodak, G., Mutchnik, S., Nakamoto, T. and Chang, C.: Immunohistochemical localization of androgen receptors with mono- and polyclonal antibodies to androgen receptor. J. Endocrinol. 126; 17-25, 1990.
21. Taylor, C. R., Shi, S-R., Chaiwun, B., Young, L., Imam, S. A. and Cote, R. J.: Strategies for improving the immunohistochemical staining of various intranuclear prognostic markers in formalin-paraffin sections: androgen receptor, estrogen receptor, progesterone receptor, p53 protein, proliferating cell nuclear antigen, and Ki-67 antigen revealed by antigen retrieval techniques. Hum. Pathol. 25; 263-270, 1107-1109, 1994.
22. Tilley, W. D., Marcelli, M., Wilson, J. D. and McPhaul, M. J.: Characterization and expression of a cDNA encoding the human androgen receptor. Proc. Natl. Acad. Sci. U.S.A. 86; 327-331, 1989.
23. de Winter, J. A. R., Janssen, P. J. A., Sleddens, H. M. E. B., Verleun-Mooijman, M. C. T., Trapman, J., Brinkmann, A. O., Santerse, A. B., Schrode, F. H. and van der Kwast, T. H.: Androgen receptor status in localized and locally progressive hormone refractory human prostate cancer. Am. J. Pathol. 144; 735-746, 1994.