Recent advances in molecular histochemical techniques: In situ hybridization and southwestern histochemistry

Journal of Electron Microscopy

Volumn 45, Issue 2, 1996, Pages 119-127

  Koji, Takehiko ^a   Nakane, Paul K ^a  

^a NAGASAKI UNIVERSITY   (Japan)

Author keywords

In situ hybridization; Oligo DNA; Southwestern histochemistry; T T dimer; Ultrastructural localization

Indexed keywords

DNA; MESSENGER RNA;

ANIMAL; CYTOCHEMISTRY; ELECTRON MICROSCOPY; GENETICS; IN SITU HYBRIDIZATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; REVIEW; SOUTHERN BLOTTING; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; BLOTTING, SOUTHERN; BLOTTING, WESTERN; DNA; HISTOCYTOCHEMISTRY; IN SITU HYBRIDIZATION; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; OLIGONUCLEOTIDE PROBES; RNA, MESSENGER;

BIOCHEMISTRY; DNA SEQUENCES; HISTOLOGY; SIGNAL ANALYSIS; TRANSCRIPTION;

CELLULARS; GENES EXPRESSION; IN-SITU HYBRIDIZATION; LOCALISATION; NONRADIOACTIVE; OLIGO-DNA; SOUTHWESTERN HISTOCHEMISTRY; SUB-CELLULAR; T-T DIMER; ULTRASTRACTURAL LOCALIZATION;

DIMERS;

EID: 0030118556     PISSN: 00220744     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023420     Document Type: Article

References (71)

1. Koji T, Nakane PK: Localization in situ of specific mRNA using thymine-thymine dimerized DNA probes. Sensitive and reliable non-radioactive in situ hybridization. Acta Pathol Jpn 40: 793-807 (1990)
2. Ruizeveld de Winter JA, Trapman J, Vermey M, Mulder E, Zegers ND, van der Kwast, TH: Androgen receptor expression in human tissues: An immunohistochemical study. J Histochem Cytochem 39: 927-936 (1991)
3. Koji T, Kobayashi N, Nakanishi Y, Yoshii A, Hashimoto S, Shibata Y, Anjiki N, Yamamoto R, Aoki A, Ueda T, Kanazawa S, Nakane PK: Immunohistochemical localization of Fas antigen in paraffin sections with rabbit antibodies against human synthetic Fas peptides. Acta Histochem Cytochem 27: 459-463 (1994)
4. Slayden OD, Koji T, Brenner RM: Microwave-stabilization enhances immunocytochemical detection of estrogen receptor in frozen sections of macaque oviduct. Endocrinology 136: 4012-4021 (1995)
5. Schmitz GG, Walter T, Seibl R, Kessler C: Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase. Anal Biochem 192: 222-231 (1991)
6. Henderson GS, Conary JT, Davidson JM, Stewart SJ, House FS, McCurley TL: A reliable method for northern blot analysis using synthetic oligonucleotide probes. BioTechniques 10: 190-197 (1991)
7. Kerkhoff E, Bister K, Klempnauer K-H: Sequence-specific DNA binding by Myc proteins. Proc Natl Acad Sci USA 88: 4323-4327 (1991)
8. Curran T, Franza RB: Fos and Jun: The AP-1 connection. Cell 55: 395-397 (1988)
9. Meyer TE, Habener JF: Cyclic adenosine 3′,5′-monophosphate response element binding protein (CREB) and related transcription-activating deoxyribonucleic acid-binding proteins. Endocrine Rev 14: 269-290 (1993)
10. Beato M: Gene regulation by steroid hormones. Cell 56: 335-344 (1989)
11. Koji T, Yamada S, Kayashima K, Nakane PK: A new approach to localize glucocorticoid receptor using DNA probe containing glucocorticoid responsive element DNA consensus sequences. Acta Histochem Cytochem 25: 681-687 (1992)
12. Koji T, Komuta K, Nozawa M, Yamada S, Nakane PK: Localization of cyclic adenosine 3′,5′-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry. J Histochem Cytochem 42: 1399-1405 (1994)
13. Guitteny A-F, Fouque B, Mougin C, Teoule R, Bloch B: Histological detection of messenger RNAs with biotinylated synthetic oligonucleotide probes. J Histochem Cytochem 36: 563-571 (1988)
14. Larsson L-I, Christensen T, Dalboge H: Detection of proopiomelanocortin mRNA by in situ hybridization, using a biotinylated oligodeoxynucleotide probe and avidin-alkaline phosphatase histochemistry. Histochemistry 89: 109-116 (1988)
15. Jirikowski GF, Ramalho-Ortigao JF, Lindl T, Seliger H: Immunocytochemistry of 5-bromo-2′-deoxyuridine labelled oligonucleotide probes. A novel technique for in situ hybridization. Histochemistry 91: 51-53 (1989)
16. Koji T, Sugawara I, Kimura M, Nakane PK: In situ localization of c-myc mRNA in HL-60 cells using non-radioactive synthetic oligodeoxynucleotide probes. Acta Histochem Cytochem 22: 295-307 (1989)
17. Dirks RW, van Gijlswijk RPM, Tullis RH, Smit AB, van Minnen J, van der Ploeg M, Raap AK: Simultaneous detection of different mRNA sequences coding for neuropeptide hormones by double in situ hybridization using FITC- and biotin-labeled oligonucleotides. J Histochem Cytochem 38: 467-473 (1990)
18. Dirks RW, van Gijlswijk RPM, Vooijs MA, Smit AB, Bogerd J, van Minnen J, Raap AK, van der Ploeg M: 3′-Endo fluorochromized and haptenized oligonucleotides as in situ hybridization probes for multiple, simultaneous RNA detection. Exp Cell Res 194: 310-315 (1991)
19. Koji T, Brenner RM: Localization of estrogen receptor messenger ribonucleic acid in rhesus monkey uterus by nonradioactive in situ hybridization with digoxigenin-labeled oligodeoxynucleotides. Endocrinology 132: 382-392 (1993)
20. Razzaque MS, Koji T, Taguchi T, Harada T, Nakane PK: In situ localization of type III and type IV collagen-expressing cells in human diabetic nephropathy. J Pathol 174: 131-138 (1994)
21. Yoshii A, Koji T, Ohsawa N, Nakane PK: In situ localization of ribosomal RNAs is a reliable reference for hybridizable RNA in tissue sections. J Histochem Cytochem 43: 321-327 (1995)
22. Komuta K, Koji T, Izumi S, Matsumoto T, Kohara N, Motojima K, Kanematsu T, Nakane PK: Expression of epidermal growth factor receptor messenger RNA in human colorectal carcinoma assessed by non-radioactive in-situ hybridization. Eur J Surg Oncol 21: 269-275 (1995)
23. Koji T, Chedid M, Rubin JS, Slayden OD, Csaky KG, Aaronson SA, Brenner RM: Progesterone-dependent expression of keratinocyte growth factor mRNA in stromal cells of the primate endometrium: Keratinocyte growth factor as a progestomedin. J Cell Biol 125: 393-401 (1994)
24. Koji T, Jinno A, Matsushime H, Shibuya M, Nakane PK: In situ hybridization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: Specific expression in germ cells at stages around meiotic cell division. Cell Biochem Funct 10: 273-279 (1992)
25. Ehara H, Koji T, Deguchi T, Yoshii A, Nakano M, Nakane PK, Kawata Y: Expression of estrogen receptor in diseased human prostate assessed by non-radioactive in situ hybridization and immunohistochemistry. Prostate: 27: 304-313 (1995)
26. Ueki T, Koji T, Tamiya S, Nakane PK, Tsuneyoshi M: Expression of both basic fibroblast growth factor and fibroblast growth factor receptor indicates a poor prognosis in human gastric carcinoma. J Pathol 177: 353-361 (1995)
27. Koji T, Moriuchi T, Nakane PK: Improved tissue preparation for in situ localization of specific mRNA using non-radioactive DNA probes: Effects of protease digestion and probe size on signal detection in frozen and paraffin sections of rat pituitary glands. Acta Histochem Cytochem 21: 187-200 (1988)
28. Adams JC: Heavy metal intensification of DAB-based HRP reaction products. J Histochem Cytochem 29: 775 (1981)
29. Razzaque MS, Koji T, Kawano H, Harada T, Nakane PK, Taguchi T: Glomerular expression of type III and type IV collagens in benign nephrosclerosis: Immunohistochemical and in situ hybridization study. Pathol Res Pract 190: 493-199 (1994)
30. Mullink H, Vos W, Jiwa M, Horstman A, van der Valk P, Walboomers JMM, Meijer CJLM: Application and comparison of silver intensification methods for the diaminobenzidine and diaminobenzidine-nickel endproduct of the peroxidation reaction in immunohistochemistry and in situ hybridization. J Histochem Cytochem 40: 495-504 (1992)
31. Teo CG, Griffin BE: Visualization of single copies of the Epstein-Barr virus genome by in situ hybridization. Anal Biochem 186: 78-85 (1990)
32. Miyazaki M, Nikolic-Paterson DJ, Endoh M, Nomoto Y, Sakai H, Atkins RC, Koji T: A sensitive method of non-radioactive in situ hybridization for mRNA localization within human renal biopsy specimens: Optimization of non-radioactive in situ hybridization with digoxigenin labeled oligonucleotides. Internal Med 33: 87-91 (1994)
33. Kerstens HMJ, Poddighe PJ, Hanselaar AGJM: A novel in situ hybridization signal amplification method based on the deposition of biotinylated tyramine. J Histochem Cytochem 43: 347-352 (1995)
34. Ried T, Baldini A, Rand TC, Ward DC: Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial flourescence and digital imaging microscopy. Proc Natl Acad Sci USA 89: 1388-1392 (1992)
35. Speel EJM, Jansen MPHM, Ramackers FCS, Hopman AHN: A novel triple-color detection procedure for brightfield microscopy, combining in situ hybridization with immunocytochemistry. J Histochem Cytochem 42: 1299-1307 (1994)
36. Durrant I, Brunning S, Eccleston L, Chadwick P, Cunningham M: Fluorescein as a label for non-radioactive in situ hybridization. Histochem J 27: 94-99 (1995)
37. Lorimier P, Lamarcq L, Labat-Moleur F, Bethler GR, Stoebner P: Enhanced chemiluminescence: A high-sensitivity detection system for in situ hybridization and immunohistochemistry. J Histochem Cytochem 41: 1591-1597 (1993)
38. Bijl JJ, Rieger E, van Oostveen JW, Meijer CJLM, Oudejans CBM, Walboomers JMM: Quantification of biotinylated RNA probes for in situ hybridization using chemiluminescence. Histochemistry 102: 77-82 (1994)
39. Golden JF, West SS, Echols CK. Shingleton HM: Quantitative flourescence spectrophotometry of acridine orange-stained unfixed cells. Potential for automated detection of human uterine cancer. J Histochem Cytochem 24: 315-321 (1976)
40. Schulte EKW, Lyon HO, Hoyer PE: Simultaneous quantification of DNA and RNA in tissue sections: A comparative analysis of the Methyl Green-Pyronin technique with Gallacyanin chromalum and Feulgen procedures using image cytometry. Histochem J 24: 305-310 (1992)
41. Chan Y-L, Olvera J, Wood IG: The structure of rat 28S ribosomal ribonucleic acid inferred from the sequence of nucleotides in a gene. Nucleic Acids Res 11: 7819-7831 (1983)
42. Kordylewski L, Ambler SK, Doyle DD: Quantification of ANP mRNA in primary cultures of adult rat atrial myocytes by image processing: In situ hybridization to multiple parallel sample using single-stranded cDNA probes. J Histochem Cytochem 43: 481-488 (1995)
43. Nakane PK, Koji T, Shin M: In situ hybridization using T-T dimerized non-radioactive probes. In: Cancer chemotherapy: Challenges for the future, vol 6. Tokyo: Excerpta Medica, 46-53 (1991)
44. Larsson L-I, Traasdahl B, Hougaard DM: Quantitative non-radioactive in situ hybridization. Model studies on pituitary proopiomelanocortin cells after adrenalectomy. Histochemistry 95: 209-215 (1991)
45. Sundell CL, Singer RH: Requirement of microfilaments in sorting of actin messenger RNA. Science 253: 1275-1277 (1991)
46. Wilhelm JE, Vale RD: RNA on the move: The mRNA localization pathway. J Cell Biol 123: 269-274 (1993)
47. Rotundo RL: Nucleus-specific translation and assembly of acetylcholinesterase in multinucleated muscle cells. J Cell Biol 110: 715-719 (1990)
48. Larsson L-I, Hougaard DM: Combined non-radioactive detection of peptide hormones and their mRNAs in endocrine cells. Histochemistry 96: 375-380 (1991)
49. Hesketh JE, Pryme IF: Interaction between mRNA, ribosomes and the cytoskeleton. Biochem J 277: 1-10 (1991)
50. Taneja KL, Lifshitz LM, Fay FS, Singer RH: Poly(A) RNA codistribution with microfilaments: Evaluation by in situ hybridization and quantitative digital imaging microscopy. J Cell Biol 119: 1245-1260 (1992)
51. Geuskens M, May E: Ultrastructural localization of SV40 viral DNA in cells, during lytic infections, by in situ molecular hybridization. Exp Cell Res 87: 175-185 (1974)
52. Hutchison NJ, Langer-Safer PR, Ward DC, Hamkalo BA: In situ hybridization at the electron microscope level: Hybrid detection by autoradiography and colloidal gold. J Cell Biol 95: 609-618 (1982)
53. Thiry M, Thiry-Blaise L: Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy. Nucleic Acids Res 19: 11-15 (1991)
54. Jimenez-Garcia LF, Segura-Valdez ML, Ochs RL, Echeverria OM, Vazquez-Nin GH, Busch H: Electron microscopic localization of ribosomal DNA in rat liver nucleoli by nonisotopic in situ hybridization. Exp Cell Res 207: 220-225 (1993)
55. Dirks RW, van Dorp AGM, van Minnen J, Fransen JAM, van der Ploeg M, Raap AK: Electron microscopic detection of RNA sequences by non-radioactive in situ hybridization in the mollusk Lymnaca stagnalis. J Histochem Cytochem 40: 1647-1657 (1992)
56. Yi J, Michel O, Sassy-Prigent C, Chevalier J: Electron microscopic location of mRNA in the rat kidney: Improved post-embedding in situ hybridization. J Histochem Cytochem 43: 801-809 (1995)
57. Bassell GJ, Powers CM, Taneja KL, Singer RH: Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts. J Cell Biol 126: 863-876 (1994)
58. Trembleau A, Bloom FE: Enhanced sensitivity for light and electron microscopic in situ hybridization with multiple simultaneous non-radioactive oligodeoxynucleotide probes. J Histochem Cytochem 43: 829-841 (1995)
59. Dorries U, Bartsch U, Nolte C, Roth J, Schachner M: Adaptation of a non-radioactive in situ hybridization method to electron microscopy: Detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies. Histochemistry 99: 251-262 (1993)
60. Egger D, Troxler M, Bienz K: Light and electron microscopic in situ hybridization: Non-radioactive labeling and detection, double hybridization, and combined hybridization-immunocytochemistry. J Histochem Cytochem 42: 815-822 (1994)
61. Sibon OCM, Cremers FFM, Humbel BM, Boonstra J, Verkleij AJ: Localization of nuclear RNA by pre- and post-embedding in situ hybridization using different gold probes. Histochem J 27: 35-45 (1995)
62. Nakane PK, Matsumura H, Koji T: In situ hybridization using T-T dimerized non-radioactive probes. In: Tabuchi K, ed. Proceedings of the 8th Nikko brain tumor conference. Tokyo: Springer-Verlag, 52-62 (1990)
63. Liscia DS, Doberty PJ, Smith GH: Localization of α-casein gene transcription in sections of epoxy resin-embedded mouse mammary tissues by in situ hybridization. J Histochem Cytochem 36: 1503-1510 (1988)
64. Binder M, Tourmente S, Roth J, Renaud M, Gehring WJ: In situ hybridization at the electron microscope level: Localization of transcripts on ultrathin sections of Lowicryl K4M-embedded tissue using biotinylated probes and protein A-gold complexes. J Cell Biol 102: 1646-1653 (1986)
65. Nakane PK, Koji T, Terasaki T, Izumi S: In situ localization of mRNA for peptide hormones. In: Hoshino K, ed. Prolactin gene family and its receptor. Tokyo: Elsevier Sci Publisher BV. 299-305 (1988)
66. Tully DB, Cidlowski JA: pBR322 contains glucocorticoid regulatory element DNA consensus sequences. Biochem Biophys Res Commun 144: 1-10 (1987)
67. Evans RM: The steroid and thyroid hormone receptor. Science 240: 889-895 (1988)
68. Miskimins WK, Roberts M, McClelland A, Ruddle FH: Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene. Proc Natl Acad Sci USA 82: 6741-6744 (1985)
69. Kato Y, Kato T, Ezashi T, Inoue K: In situ binding for detection of proteins that bind to regulatory elements. Biochem Biophys Res Commun 195: 963-968 (1993)
70. Gentilomi G, Musiani M, Zerbini M, Gibellini G, Gallinella G, Venturoli S: Double in situ hybridization for detection of Herpes simplex virus and Cytomegalovirus DNA using non-radioactive probes. J Histochem Cytochem 40: 421-425 (1992)
71. Martinez A, Miller M, Quinn K, Unswoth EJ, Ebina M, Cuttitta F: Non-radioactive localization of nucleic acids by direct in situ PCR and in situ RT-PCR in paraffin-embedded sections. J Histochem Cytochem 43: 739-747 (1995)