A proton magnetic resonance spectroscopy study of aging and transformed human fibroblasts

Experimental Gerontology

Volumn 31, Issue 6, 1996, Pages 669-686

  Rutter, Allison ^a   Mackinnon, Wanda B ^a   Huschtscha, Lily I ^b   Mountford, Carolyn E ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b WESTMEAD HOSPITAL   (Australia)

Author keywords

aging; human lung fibroblasts; magnetic resonance spectroscopy; transformation

Indexed keywords

CHOLESTEROL; CHOLINE; LIPID;

AGING; ARTICLE; CELL IMMORTALIZATION; CELL MEMBRANE; CELL TRANSFORMATION; HUMAN; HUMAN CELL; LUNG FIBROBLAST; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; SENESCENCE; SIMIAN VIRUS 40;

CELL AGING; CELL LINE, TRANSFORMED; CHOLESTEROL; CHOLINE; FIBROBLASTS; HUMANS; MAGNETIC RESONANCE SPECTROSCOPY; MEMBRANE LIPIDS; SIMIAN VIRUS 40;

SIMIAE; SIMIAN VIRUS; SIMIAN VIRUS 40; SV40 VIRUS;

EID: 0030296621     PISSN: 05315565     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0531-5565(96)00076-9     Document Type: Article

References (52)

1. AGRIS, P.F. and CAMPBELL, I.D. Proton nuclear magnetic resonance of intact Friend Leukemia cells: Phosphorylcholine increase during differentiation. Science 216, 1325-1327, 1982.
2. AURELI, T., CAPUANI, G., DI COCCO, M. E., RICCIOLINI, R., GIRARDI, O. RAMACCI, T., and MICCHELI, A. Changes in brain lipid composition during aging: Effect of long-term acetyl-L-carnitine treatment. Q. Magn. Res. Bio. Med. 1, 47-52, 1994.
3. BLONDAL, J.A., DICK, J.E., and WRIGHT, J.A. Membrane glycoprotein changes during the senescence of normal human diploid fibroblasts in culture. Mech. Ageing Dev. 30, 273-283, 1985.
4. BUSSE, S.C., MANN, P.L., and GRIFFEY, R.H. Senescence-induced alteration in cell surface carbohydrates correlated using proton NMR spectroscopy and a lectin-based affinity-binding assay. Biochim. Biophys. Acta 984, 183-187, 1989.
5. 1H NMR spectra of a myeloma cell line correlates with the induced formation of cytoplasmic lipid droplets. Magn. Reson. Med. 29, 546-550, 1993.
6. CAMPISI, J. Replicative senescence - An old live's tale. Cell 84, 497-500, 1996.
7. CHEN, X., HAN, X., and GROSS, R.W. Dynamics of binary mixtures of plasmenycholine/arachidonic acid and phosphatidyl choline/arachidonic acid - A study using fluorescence and NMR spectroscopy. Biochim. Biophys. Acta 1149, 241-248, 1993.
8. CRISTOFALO, V.J. and PIGNOLO, R.J. Molecular markers of senescence in firboblast-like cultures. Exp. Genont. 31, 111-123, 1996.
9. 13P NMR spectroscopy. J. Biol. Chem. 262, 14875-14878, 1987.
10. DELIKATNY, E.J., HULL, W.E., and MOUNTFORD, C.E. The effect of altering time domains and window functions in two-dimensional proton COSY spectra of biological specimens. J. Magn. Res. 94, 563-573, 1991.
11. DELIKATNY, E.J., RUSSELL, P., HUNTER, J.C., HANCOCK, R., ATKINSON, K.H., van HAAFTEN-DAY, C., and MOUNTFORD, C.E. Proton MR and human cervical neoplasia: Ex vivo spectroscopy allows distinction of invasive carcinoma of the cervix from carcinoma in situ and other preinvasive lesions. Radiology 188, 791-796, 1993.
12. ETHIER, M.F., HICKLER, R.B., and SAUNDERS, R.H. Cholesterol and cholesteryl ester concentration in different size classes of cultured human fibroblasts: Effect of in vitro aging. Mech. Ageing Dev. 20, 165-174, 1982.
13. FREITAS, I., PORTIGGIA, P., BARNI, S., BERTONE, U., PARENTE, M., NAVARINA A., RAVETA G., GERZELI, G., and STOWARD, P. Histochemical probes for the detection of hypoxic tumour cells. Anticancer Res. 10, 613-622, 1990.
14. GALONS, J.P., FANTINI, J., VION-DURY, J., COZZONE, P.J., and CANIONI, P. Metabolic changes in undifferentiated and differentiated human colon adenocarcinoma cells studied by multinuclear magnetic resonance spectroscopy. Biochimie 71, 949-961, 1989.
15. GILL, S.S., THOMAS, D.G.T., VAN BRUGGEN, N., GADIAN, D.G., PEDEN, C.J., BELL, J.D., COX, I.J., MENON, D.K., ILES, R.A., BRYANT, D.J., and COUTTS, G.A. Proton MR spectroscopy of intracranial tumours: In vivo and in vitro studies. J. Comput. Assist. Tomogr. 14(4), 497-504, 1990.
16. GIRARDI, A.J., JENSEN, F.C., and KOPROWSKI, H. SV40-induced transformation of human diploid cells: Crisis and recovery. J. Cell. Comp. Physiol. 65, 69-84, 1965.
17. HAYFLICK, L. The limited in vitro lifetime of human diploid cell strains. Exp. Cell Res. 37, 614-636, 1965.
18. HAYFLICK, L. Recent advances in the cell biology of ageing. Mech. Ageing Dev. 14, 59-79, 1980.
19. HAYFLICK, L. Cell aging. In: Annual Review of Gerontology and Geriatrics, Eisdorfer, C. (Editor), Chapter 2, Vol. 1, pp.26-67, Springer Publishing Company, New York, 1980.
20. HAYFLICK, L., and MOORHEAD, P.S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25, 585-621, 1961.
21. HIRAKAWA, T., MARUYAMA, K., KOHL, N.E., KODAMA, T., and RULEY, H.E. Massive accumulation of neutral lipids in cells conditionally transformed by an activated H-RAS oncogene. Oncogene 6, 289-295, 1991.
22. HOLLIDAY, R. The limited proliferation of cultured human diploid cells: Regulation of senescence? J. Gerontol. 45, 36-41, 1990.
23. HOLLIDAY, R., and TARRANT, G.M. Altered enzymes in ageing human fibroblasts. Nature 238, 26-30, 1972.
24. 1H MR spectrum in Murine T Cells and B Cells occurs in G1 phase of the cell cycle. Magon. Reson. Med. 16, 1-8, 1990.
25. HOWARD, B.V. and KRITCHEVSKY, D. The lipids of normal diploid (W1-38) and SV40-transformed human cells. Int. J. Cancer 4, 393-402, 1969.
26. HUSCHTSCHA, L.I. and HOLLIDAY, R. Limited and unlimited growth of SV40-transformed cells from human diploid MRC-5 fibroblasts. J. Cell. Sci. 63, 77-99, 1983.
27. KING, N.J.C., WARD, M.H., and HOLMES, K.T. Magnetic resonance studies of murine macrophages. Proliferation is not a prerequisite for acquisition of an 'activated' high resolution spectrum. FEBS J. 287, 97-101, 1991.
28. KOLI, K. and KESKI-OJA, J. Cellular senescence. Ann. Med. 24, 313-318, 1992.
29. LEAN, C.L., MACKINNON, W.B., DELIKATNY, E.J., WHITEHEAD, R.H., and MOUNTFORD, C.E. Cell-surface fucosylation and magnetic resonance spectroscopy charcterization of human malignant colorectal cell. Biochemistry 31, 11095-11105, 1992.
30. 1H MRS: Correlation with histopathology. MRM 30, 525-533, 1993.
31. MACKINNON, W.B., MAY, G.L., and MOUNTFORD, C.E. Esterified cholesterol and triglyceride are present in plasma membranes of Chinese hamster ovary cells. Eur. J. Biochem. 205, 827-839, 1992.
32. 1H magnetic resonance spectroscopy. Int. J. Cancer 59, 248-261, 1994.
33. MALONE, M.J. and SZOKE, M.C. Neurochemical studies in aging brain. I. Structural changes in myelin lipids. J. Gerontol. 37, 262-267, 1982.
34. MARTIN, G.M., SPRAGUE, C.A., and EPSTEIN, C.J. Replicative life-span of cultivated human cells. Effects of donor's age, tissue, and genotype. Lab. Invest. 23, 86-92, 1970.
35. MAY, G.L., WRIGHT, L.C., HOLMES, K.T., WILLIAMS, P.G., SMITH, I.C.P., WRIGHT, P.E., FOX, R.M., and MOUNTFORD, C.E. Assignment of methylene proton resonances in NMR spectra of embryonic and transformed cells to plasma membrane triglyceride. J. Biol. Chem. 261, 3048-3053, 1986.
36. MAY, G.L., WRIGHT, L.C., DYNE, M., MACKINNON, W.B., FOX, R.M., and MOUNTFORD, C.E. Plasma membrane lipid composition of vinblastine sensitive and resistant human leukaemic lymphoblasts. Int. J. Cancer 42, 728-733, 1988.
37. MAY, G.L., DUNLOP, L.C., SZYELLMA K., BERNDT, M.C., and SORRELL, T.C. GMP-140 p-selectin inhibits human neutrophil activation by lipopolysaccharide: Analysis by proton magnetic resonance spectroscopy. Biochem. Biophys. Res. Commun. 183, 1062-1069, 1992.
38. MIRANDA, A.F., BABISS, L.E., and FISHER, P.B. Transformation of human skeletal muscle cells by Simian virus 40. Proc. Natl. Acad. Sci. USA 80, 6581-6585, 1983.
39. MOUNTFORD, C.E., GROSSMAN, G., REID, G., and FOX, R.M. Characterization of transformed cells and tumors by proton nuclear magnetic resonance spectroscopy. Cancer Res. 42, 2270-2276, 1982a.
40. MOUNTFORD, C.E., GROSSMAN, G., HAMPSON, A.W., and HOLMES, K.T. Influenza virus: An NMR study of mechanisms involved in infection. Biochim. Biophys. Acta 720, 65-72, 1982b.
41. MOUNTFORD, C.E., MACKINNON, W.B., BLOOM, M., BURNELL, E.E., and SMITH, I.C.P. NMR methods for characterizing the state of the surfaces of complex mammalian cells. J. Biochem. Biophys. Methods 9, 323-330, 1984.
42. MOUNTFORD, C.E. and WRIGHT, L.C. Organisation of lipids in the plasma membranes of malignant and stimulated cells: A new model. Trends Biosci. 13, 172-177, 1988.
43. MOUNTFORD, C.E., LEAN, C.L., MACKINNON., W.B., and RUSSELL, P. The use of proton MR in cancer pathology. In: Annual Reports on NMR Spectroscopy, Webb, G.A. (Editor), pp. 173-215, Academic Press, New York, 1993.
44. 13C nuclear magnetic resonance spectra of the lipids in normal and SV40 virus-transformed hamster embryo fibroblast membranes. Biochim. Biophys. Acta 382, 311-321, 1975.
45. PEREIRA-SMITH, O.M., FISHER, F., and SMITH, J.R. Senescent and quiescent cell inhibitors of DNA synthesis: Membrane-associated proteins. Exp. Cell Res. 160, 297-306, 1985.
46. POLGAR, P., TAYLOR, L., and BROWN, L. Plasma membrane associated metabolic parameters and the aging of human diploid fibroblasts. Mech. Ageing Dev. 7, 151-160, 1978.
47. ROSENBERGER, R.F., GOUNARIS, E., and KOLETTAS, E. Mechanisms responsible for the limited lifespan and immortal phenotypes in cultured mammalian cells. J. Theor. Biol. 148, 383-392, 1991.
48. SCHROEDER, F., GOETZ, I., and ROBERTS, E. Age-related alterations in cultured human fibroblast membrane structure and function. Mech. Ageing Dev. 25, 365-389, 1984.
49. SMITH, J.R. DNA synthesis inhibitors in cellular senescence. J. Gerontol. 45, 32-35, 1990.
50. STANULIS-PRAEGER, B.M. Cellular senescence revisited: A review. Mech. Ageing Dev. 38, 1-48, 1987.
51. 1H-NMR. Biochim. Biophys. Acta 1054, 189-206, 1990a.
52. 1H-NMR of acid extracts. Biochim. Biophys. Acta 1054, 181-197, 1990b.