Purification and characterization of the hexokinase from Schistosoma mansoni expressed in Escherichia coli

Protein Expression and Purification

Volumn 8, Issue 3, 1996, Pages 374-380

  Armstrong, Robert L ^a,c   Wilson, John E ^a   Shoemaker, Charles B ^b  

^a Michigan State University ^*  (United States)

^b HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

^c ALBION COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030293976     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0113     Document Type: Article

References (30)

1. 1. Wilson, J. E. (1995) Hexokinases. Rev. Physiol. Biochem. Pharmacol. 126, 65-198.
2. 2. Bork, P., Sander, C., and Valencia, A. (1993) Convergent evolution of similar enzymatic function on different protein folds: The hexokinase, ribokinase, and galactokinase families of sugar kinases. Prot. Sci. 2, 31-40.
3. 3. Colowick, S. P. (1973) The hexokinases, in "The Enzymes" (Boyer, P. D., Ed.), Vol. 9, pp. 1-48, Academic Press, New York.
4. 4. Mochizuki, Y. (1981) Evolutionary aspects of lower deuterostom-ian hexokinases. Comp. Biochem. Physiol. 70B, 745-751.
5. 5. Yanagawa, H.-A. (1978) Tissue distribution, purifications, and properties of multiple forms of hexokinase in the silkworm, Bombyx mori. Insect Biochem. 8, 293-305.
6. 6. Tielens, A. G. M., van den Heuvel, J. M., van Mazijk, H. J., Wilson, J. E., and Shoemaker, C. B. (1994) The 50-kDa glucose 6-phosphate-sensitive hexokinase of Schistomsoma mansoni. J. Biol. Chem. 269, 24736-24741.
7. 7. Katzen, H. M., and Schimke, R. T. (1965) Multiple forms of hexokinase in the rat: Tissue distribution, age dependency, and properties. Proc. Natl. Acad. Sci. USA 54, 1218-1225.
8. 8. Gonzalez, C. Ureta, T., Babul, J., Rabajille, E., and Niemeyer, H. (1967) Characterization of isoenzymes of adenosine triphosphate D-hexose 6-phosphotransferase from rat liver. Biochemistry 6, 460-468.
9. 9. White, T. K, and Wilson, J. E. (1989) Isolation and characterization of the discrete N-and C-terminal halves of rat brain hexokinase: Retention of full catalytic activity in the isolated C-terminal half. Arch. Biochem. Biophys. 259, 402-411.
10. 2-and COOH-terminal halves. J. Biol. Chem. 271, 1849-1852.
11. 11. Tsai, H., and Wilson, J. E. (1996) Functional organization of mammalian hexokinases: Both N-and C-terminal halves of the rat Type II isozyme possess catalytic sites. Arch. Biochem. Biophys. 329, 7-13.
12. 12. Shoemaker, C. B., Reynolds, S. R., Wei, G., Tielens, A. G. M., and Harn, D. A. (1995) Schistosoma mansoni hexokinse: cDNA cloning and immunogenicity studies. Exp. Parasitol. 80, 36-45.
13. 13. Ferrari, R. A., Mandelstam, P., and Crane, R. K. (1959) 1,5-Anhydro-D-glucitol 6-phosphate and its use for the specific inhibition of the hexokinase reaction in tissue homogenates. Arch. Biochem. Biophys. 80, 372-377.
14. 14. Wilson, J. E. (1989) Rapid purification of mitochondrial hexokinase from rat brain by a single affinity chromatography step on Affi-Gel Blue. Prep. Biochem. 19, 13-21.
15. 15. Perrella, F. W. (1988) EZ-Fit: A practical curve-fitting microcomputer program for the analysis of enzyme kinetic data on IBM-PC compatible computers. Anal. Biochem. 174, 437-447.
16. 16. Brown, R. E., Jarvis, K. L., and Hyland, K. J. (1989) Protein measurement using bicinchoninic acid: Elimination of interfering substances. Anal. Biochem. 180, 136-139.
17. 17. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
18. f-measurement, and optimized procedure. Anal. Biochem. 78, 459-482.
19. 19. Towbin, H., Staehelin, T., and Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
20. 20. Taketa, K., Ichikawa, E., and Hanada, T. (1986) A tetrazolium method for staining peroxidase labels in blotting assays. J. Immunol. Methods 95, 71-77.
21. 21. Chou, A. C., and Wilson, J. E. (1972) Purification and properties of rat brain hexokinase. Arch. Biochem. Biophys. 151, 48-55.
22. 22. Needels, D. L., and Wilson, J. E. (1983) The identity of hexoki-nase activities from mitochondrial and cytoplasmic fractions of rat brain homogenates. J. Neurochem. 40, 1134-1143.
23. 23. Qadri, S. S., and Easterby, J. S. (1980) Purification of skeletal muscle hexokinase by affinity elution chromatography. Anal. Biochem. 105, 299-303.
24. 24. Wilson, J. E. (1985) Regulation of mammalian hexokinase activity, in "Regulation of Carbohydrate Metabolism" (Beitner, R., Ed.), pp. 45-85, CRC Press, Boca Raton, FL.
25. 25. Crane, R. K., and Sols, A. (1954) The non-competitive inhibition of brain hexokinase by glucose-6-phosphate and related compounds. J. Biol. Chem. 210, 597-606.
26. 26. Wilson, J. E. (1979) Ligand-induced conformational changes of rat brain hexokinase: Their role in determining the substrate specificity for hexoses and inhibitory effectiveness of hexose 6-phosphates. Arch. Biochem. Biophys. 196, 79-87.
27. 27. Chothia, C., and Lesk, A. M. (1986) The relation between the divergence of sequence and structure in proteins. EMBO J. 5, 823-826.
28. 28. Chou, A. C., and Wilson, J. E. (1974) A study of the sulfhydryl groups of rat brain hexokinase. Arch. Biochem. Biophys. 163, 191-199.
29. 29. Redkar, V. D., and Kenkare, U. W. (1975) Effect of ligands on the reactivity of essential sulfhydryls in brain hexokinase. Possible intereaction between substrate binding sites. Biochemistry 14, 4704-4712.
30. 30. Harrison, R. (1985) Crystallographic Refinement of Two Isozymes of Yeast Hexokinase and the Relationship of Structure to Function, Ph.D. Thesis, Yale University, New Haven, CT.