Hepatitis C virus infection and mixed cryoglobulinaemia: Assessment of HCV RNA copy numbers in supernatant, cryoprecipitate and non-liver cells

Journal of Viral Hepatitis

Volumn 3, Issue 6, 1996, Pages 285-292

  Manzin, A ^a   Solforosi, L ^a   Candela, M ^a   Cherubini, G ^a   Piccinini, G ^a   Brugia, M ^a   Gabrielli, A ^a   Clementi, M ^b  

^a UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

^b UNIVERSITY OF TRIESTE   (Italy)

Author keywords

Competitive reverse transcription polymerase chain reaction; HCV RNA copy number; Hepatitis C virus; Mixed cryoglobulinaemia

Indexed keywords

VIRUS RNA;

AGED; ARTICLE; BLOOD; BONE MARROW CELL; CRYOGLOBULINEMIA; FEMALE; GENE DOSAGE; GENETICS; GENOTYPE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; ISOLATION AND PURIFICATION; MALE; MIDDLE AGED; MONONUCLEAR CELL; PATHOLOGY; VIROLOGY;

AGED; BONE MARROW CELLS; CRYOGLOBULINEMIA; FEMALE; GENE DOSAGE; GENOTYPE; HEPACIVIRUS; HEPATITIS C; HUMANS; LEUKOCYTES, MONONUCLEAR; MALE; MIDDLE AGED; RNA, VIRAL;

EID: 0030278275     PISSN: 13520504     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2893.1996.tb00100.x     Document Type: Article

References (50)

1. Alter HJ, Purcell RH, Shih JW et al. Detection of antibody to hepatitis C virus in prospectively followed transfusion recipients with acute and chronic non-A, non-B hepatitis. N Engl J Med 1989; 321: 1494-1500.
2. Saito I, Miyamura T, Ohbayashi A et al. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci USA 1990; 87: 2059-2061.
3. Simons JN, Tami J, Pilot-Matias TJ et al. Identification of two flavivirus-like genomes in the GB hepatitis agent. Proc Natl Acad Sci USA 1995; 92: 3401-3405.
4. Yoshiba M, Okamoto H, Mishiro S. Detection of the HBV-C hepatitis virus genome in serum from patients with fulminant hepatitis of unknown aetiology. Lancet 1995; 346: 1131-1132.
5. Johnson RJ, Gretch DR, Yamabe H et al. Membranoproliferative glomerulonephritis associated with hepatitis C virus infection. N Engl J Med 1993; 328: 465-470.
6. Lenzi M, Ballardini G, Fusconi M et al. Type 2 autoimmune hepatitis and hepatitis C virus infection. Lancet 1990; ii: 258-259.
7. Pascual M, Perrin L, Giostra E, Schifferli JA. Hepatitis C virus in patients with cryoglobulinemia type II. J Infect Dis 1990; 162: 569-570.
8. Lo Spalluto J, Dorward B, Miller Jr W, Ziff M. Cryoglobulinemia based on interaction between a gamma macroglobulin and 7S gamma globulin. Am J Med 1962; 32: 142-147.
9. Meltzer M, Franklin EC, Elias K, McCluskey M, Cooper N. Cryoglobulinemia - A clinical and laboratory study. II. Cryoglobulins with rheumatoid factor activity. Am J Med 1966; 40: 837-856.
10. Riethmuller G, Meltzer M, Franklin EC, Miescher PA. Serum complement levels in patients with mixed (IgM-IgG) cryoglobulinemia. Clin Exp Immunol 1966; 1: 337-339.
11. Fiorini G, Bernasconi RA, Sinico R, Chianese R, Pozzi F, D'Amico G. Increased frequency of antibodies to ubiquitous viruses in essential mixed cryoglobulinemia. Clin Exp Immunol 1986; 65: 65-70.
12. Levo Y, Gorevic PD, Kassab HJ, Zucker-Franklin D, Franklin EC. Association between hepatitis B virus and essential mixed cryoglobulinemia. N Engl J Med 1977; 296; 1501-1504.
13. Wager O, Rasanen JA, Hagman A, Klemola E. Mixed cryoglobulinemia in infectious mononucleosis and cytomegalovirus mononucleosis. Int Arch Aller 1968; 34: 345-349.
14. Casato M, Taliani G, Pucillo LP, Goffredo F, Lagana B, Bonomo L. Cryoglobulinemia and hepatitis C virus. Lancet 1991; 337: 1047-1048.
15. Misiani R, Bellavita P, Fenili D et al. Hepatitis C virus infection in patients with essential mixed cryoglobulinemia. Ann Int Med 1992; 117: 573-577.
16. Lunel F, Musset L, Cacoub P et al. Cryoglobulinemia in chronic liver diseases: role of hepatitis C virus and liver damage. Gastroenterology 1994; 106: 1291-1300.
17. Gabrielli A, Manzin A, Candela M et al. Active hepatitis C virus infection in bone marrow and peripheral blood mononuclear cells from patients with mixed cryoglobulinemia. Clin Exp Immunol 1994; 97: 87-93.
18. Galli M, Zehender G, Monti G et al. Hepatitis C RNA in the bone marrow of patients with mixed cryoglobulinemia and in subjects with noncryoglobulinemic chronic hepatitis type C. J Infect Dis 1995; 171: 672-675.
19. Bouffard P, Hayashi PH, Acevedo R, Levy N, Zeldis JB. Hepatitis C virus infection is detected in a monocyte/macrophage subpopulation of peripheral blood mononuclear cells. J Infect Dis 1992; 166: 1276-1280.
20. Ferri C, Monti M, La Civita L et al. Infection of peripheral blood mononuclear cells by hepatitis C virus in mixed cryoglobulinemia. Blood 1993; 82: 3701-3704.
21. Cacoub P, Lunel Fabiani F, Musset L et al. Mixed cryoglobulinemia and hepatitis C virus. Am J Med 1994; 40: 124-132.
22. Munoz-Fernandez S, Barbado FJ, Martin-Mola E et al. Evidence of hepatitis C virus antibodies in the cryoprecipitate of patients with mixed cryoglobulinemia. J Rheumatol 1994; 21: 229-233.
23. Bichard P, Ounanian A, Girard M et al. High prevalence of hepatitis C virus RNA in the supernatant and the cryoprecipitate of patients with essential and secondary type II mixed cryoglobulinemia. J Hepatol 1994; 21: 58-63.
24. Agnello V. The aetiology of mixed cryoglobulinaemia associated with hepatitis C virus infection. Scand J Immunol 1995; 24: 179-184.
25. Pozzato G, Mazzaro C, Crovatto M et al. Low-grade malignant lymphoma, hepatitis C virus infection, and mixed cryoglobulinemia. Blood 1994; 9: 3047-3053.
26. Mazzaro C, Tulissi P, Moretti M et al. Clinical and virological findings in mixed cryoglobulinemia. J Int Med 1995; 238: 153-160.
27. Agnello VR, Chung T, Kaplan M. A role for hepatitis C virus infection in type II cryoglobulinemia. N Engl J Med 1992; 327: 1490-1495.
28. Chomczynski P, Sachi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 16: 156-159.
29. Manzin A, Bagnarelli P, Menzo S et al. Quantitation of hepatitis C virus genome molecules in plasma samples. J Clin Microbiol 1994; 32: 1939-1944.
30. Clementi M, Menzo S, Bagnarelli P, Manzin A, Valenza A, Varaldo PE. Quantitative PCR and RT-PCR in virology. PCR Methods Appl 1993; 2: 191-196.
31. Okamoto H, Sugiyama Y, Okada S et al. Typing hepatitis C virus by polymerase chain reaction with type-specific primers: application to clinical surveys and tracing infectious sources. J Gen Virol 1992; 73: 673-679.
32. Silini E, Bono F, Cerino A et al. Virological features of hepatitis C virus infection in hemodialysis patients. J Clin Microbiol 1993; 31: 2913-2917.
33. Simmonds P, Holmes EC, Cha TA et al. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol 1993; 74: 2391-2399.
34. Ferri C, Greco F, Longobardo G et al. Association between hepatitis C virus and mixed cryoglobulinemia. Clin Exp Rheumatol 1991; 9: 621-624.
35. Invernizzi F. Type II and III mixed cryoglobulinemias, rheumatoid factors, hepatitis C virus. Semin Clin Immunol 1993; 6: 21-37.
36. Bagnarelli P, Valenza A, Menzo S et al. Dynamics of molecular parameters of human immunodeficiency virus type 1 activity in vivo. J Virol 1994; 68: 2495-2502.
37. Clementi M, Menzo S, Manzin A, Bagnarelli P. Quantitative molecular methods in virology. Arch Virol 1995; 140: 1523-1539.
38. Piatak M, Saag MS, Yang LC et al. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science 1993; 259: 1749-1754.
39. Besnard NC, André PM. Automated quantitative determination of hepatitis C virus viremia by reverse transcription PCR. J Clin Microbiol 1994; 32: 1887-1893.
40. Gretch D, Corey L, Wilson J et al. Assessment of hepatitis C virus RNA levels by quantitative competitive RNA polymerase chain reaction: high-titer viremia correlates with advanced stage of disease. J Infect Dis 1994; 169: 1219-1225.
41. Hagiwara H, Hayashi N, Mita E et al. Quantitative analysis of hepatitis C virus in serum during interferon alpha therapy. Gastroenterology 1993; 104: 877-883.
42. Kato N, Yokosuka O, Hosoda K, Ito Y, Ohoto M, Omata M. Quantification of hepatitis C virus by competitive reverse transcription-polymerase chain reaction: increase of the virus in advanced liver disease. Hepatology 1993; 18: 16-20.
43. Kumar U, Thomas HC, Monjardino J. Serum HCV RNA levels in chronic HCV hepatitis measured by quantitative PCR assay: correlation with serum ALT. J Virol Methods 1994; 47: 95-102.
44. Manzin A, Solforosi L, Bianchi D et al. Viral load in samples from hepatitis C virus (HCV)-infected patients with various clinical conditions. Res Virol 1995; 146: 279-284.
45. Werner C, Joller-Jemelka HI, Fontana A. Hepatitis C virus and cryglobulinemia. N Engl J Med 1993; 328: 1122-1123.
46. Sallberg M, Ruden U, Wahren B, Magnius LO. Antigenic regions within the hepatitis C virus envelope 1 and non structural proteins: identification of an IgG 3-restricted recognition site within the envelope 1 protein. Clin Exp Immunol 1993; 91: 489-194.
47. Gabrielli A, Zhang ZX, Cherubini G et al. Differential humoral immune response against hepatitis C virus antigenic synthetic peptides in infected patients with and without mixed cryoglobulinemia. Clin Exp Immunol 1996; 105: 59-64.
48. Muller HM, Pfaff E, Goeser T, Kallinowski B, Solbach C, Theilmann L. Peripheral blood leukocytes serve as possible extrahepatic site for hepatitis C virus replication. J Gen Virol 1993; 74: 669-676.
49. Manzin A, Candela M, Paolucci S, Caniglia ML, Gabrielli A, Clementi M. Presence of hepatitis C virus (HCV) genomic RNA and viral replicative intermediates in bone marrow and peripheral blood mononucler cells from HCV-infected patients. Clin Diag Lab Immunol 1994; 1: 160-163.
50. Zignego AL, Macchia D, Monti M et al. Infection of peripheral mononuclear blood cells by hepatitis C virus. J Hepatol 1992; 15: 382-386.