Convergence of model systems for peroxisome biogenesis

Current Opinion in Cell Biology

Volumn 8, Issue 4, 1996, Pages 513-518

  Subramani, Suresh ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN;

DISORDERS OF PEROXISOMAL FUNCTIONS; HUMAN; MODEL; MOLECULAR CLONING; NONHUMAN; PEROXISOME; PRIORITY JOURNAL; PROTEIN TRANSPORT; REVIEW; SACCHAROMYCES CEREVISIAE; YEAST CELL;

SACCHAROMYCES CEREVISIAE;

EID: 0030222363     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0955-0674(96)80029-9     Document Type: Article

References (52)

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24. 24. Wiemer EA, Nuttley WM, Bertolaet BL, Li X, Francke U, Wheelock MJ, Anne UK, Johnson KR, Subramani S: Human peroxisomal targeting signal-1 receptor restores peroxisomal protein import in cells from patients with fatal peroxisomal disorders. J Cell Biol 1995, 130:51-65. This paper, together with [23••,33•], describes the cloning of the cDNA for the human PTS1 receptor. A partial human cDNA found to be homologous to the P. pastoris PAS8 gene was used to obtain additional cDNA clones and the sequence of the human PTS1R protein. Evidence is presented to show that the PTS1R gene encodes the PTS1 receptor and that this protein is essential for the import of PTS1-contain ing proteins. The expression of the receptor cDNA in the patient cell lines corrects the PTS1-specfic protein import deficiency. A patient completely lacking the PTS1R protein (but not a patient with a missense mutation in the PTS1R gene) is demonstrated to also be deficient in the PTS2 import pathway, suggesting the possibility of interactions between the two import pathways, perhaps via the receptors.
25. 25. Marzioch M, Erdmann R, Veenhuis M, Kunau WH: PAS7 encodes a novel yeast member of the WD-40 protein family essential for import of 3-oxoacyl-CoA thiolase, a PTS2-containing protein, into peroxisomes. EMBO J 1994, 13:4908-4918.
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36. 36. Spong AP, Subramani S: Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. J Cell Biol 1993, 123:535-548.
37. 37. Waterham HR, De Vries Y, Russell KA, Xie W, Veenhuis M, Cregg JM: The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1. Mol Cell Biol 1996, 16:2527-2536. The gene described in this paper appears to be the homolog of a gene, PAF-1, which is involved in a human peroxisomal disorder [34].
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49. 49. Monosov EZ, Wenzel TJ, Luers GH, Heyman JA, Subramani S: Labeling of peroxisomes with green fluorescent protein in living P. pastoris cells. J Histochem Cytochem 1996, in press. Demonstrates the targeting of the GFP reporter to peroxisomes, and describes the use of this targeting as the first vital stain for peroxisomes. The coupling of the fluorescence of GFP to the photo-oxidation of diaminobenzidine allows the localization of GFP derivatives using electron microscopy. This extends the use of the GFP marker. This paper also describes other novel uses of the GFP derivatives for studies in the peroxisome field.
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52. 52. Wiemer EAC, Lüers G, Faber KN, Wenzel T, Veenhuis M, Subramani S: Isolation and characterization of Pas2p, a peroxisomal membrane protein essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. J Biol Chem 1996, in press.