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An innovative use of the amino acid contents of retinal neurons as a means of sorting them. High-resolution immunohistochemistry was combined with quantitative video analysis.
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Marc RE, Murry RF, Basinger SF. Pattern recognition of amino acid signatures in retinal neurons. J Neurosci. 15:1995;5106-5129 An innovative use of the amino acid contents of retinal neurons as a means of sorting them. High-resolution immunohistochemistry was combined with quantitative video analysis.
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of outstanding interest. The goal was to count the major cell classes in rabbit retina without the use of any immunohistochemistry. This was done by high resolution light microscopy of thin serial sections of the retina. The results were verified by electron microscopy. The relative fractions of the different cell types were remarkably constant from animal to animal and from preparation to preparation. This means that the base of reference thus generated can be used to translate results between different histochemical preparations.
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Strettoi E, Masland RH. The organization of the inner nuclear layer of the rabbit retina. of outstanding interest J Neurosci. 15:1995;875-888 The goal was to count the major cell classes in rabbit retina without the use of any immunohistochemistry. This was done by high resolution light microscopy of thin serial sections of the retina. The results were verified by electron microscopy. The relative fractions of the different cell types were remarkably constant from animal to animal and from preparation to preparation. This means that the base of reference thus generated can be used to translate results between different histochemical preparations.
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Wässle H, Boycott BB, Röhrenbeck J. Horizontal cells in the monkey retina: cone connections and dendritic network. Eur J Neurosci. 1:1989;421-435.
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of outstanding interest. of special interest. Confirms beyond any doubt that horizontal cells cannot create chromatic opponency in retinal ganglion cells of the monkey retina. Monkey retinae were maintained in vivo and the cells were penetrated after visualization using fluorescent dyes. The chromatic responses of the cells were studied and the cells were then injected with markers. The cone connectivity of the horizontal cells was correlated with the physiological responses. (See also [17].)
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of outstanding interest Dacey DM, Lee BB, Stafford DK, Pokorny J, Smith VC. Horizontal cells of the primate retina: cone specificity without spectral opponency. of special interest Science. 271:1996;656-658 Confirms beyond any doubt that horizontal cells cannot create chromatic opponency in retinal ganglion cells of the monkey retina. Monkey retinae were maintained in vivo and the cells were penetrated after visualization using fluorescent dyes. The chromatic responses of the cells were studied and the cells were then injected with markers. The cone connectivity of the horizontal cells was correlated with the physiological responses. (See also [17].).
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Dacey, D.M.1
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of special interest. Perspective on the paper by Dacey et al. [15]. Reviews current controversies about color pathways, with emphasis on the relationship between retinal coding and central color processing.
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of special interest Masland RH. Unscrambling color vision. Science. 271:1996;616-617 Perspective on the paper by Dacey et al. [15]. Reviews current controversies about color pathways, with emphasis on the relationship between retinal coding and central color processing.
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Boycott, B.B.1
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of special interest. This paper continues Wässle and colleagues' study of retinal bipolar cells. It should be read together with other papers [6-8,23] that report a thorough and systematic investigation of the bipolar cells of the macaque monkey. A wide range of techniques were used, and the results are carefully and thoughtfully interpreted. Most of them are presented quantitatively. This body of work is required reading for any serious student of the retina.
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Euler T, Wässle H. Immunocytochemical identification of cone bipolar cells in the rat retina. of special interest J Comp Neurol. 361:1995;461-478 This paper continues Wässle and colleagues' study of retinal bipolar cells. It should be read together with other papers [6-8,23] that report a thorough and systematic investigation of the bipolar cells of the macaque monkey. A wide range of techniques were used, and the results are carefully and thoughtfully interpreted. Most of them are presented quantitatively. This body of work is required reading for any serious student of the retina.
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J Comp Neurol
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in press
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Strettoi E, Masland RH. The number of unidentified amacrine cells in the rabbit's retina. Proc Natl Acad Sci USA. 1996;. in press.
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Meister M, Wong ROL, Baylor DA, Shatz CJ. Synchronous burst of action potentials in ganglion cells of the developing mammalian retina. Science. 2:1991;939-943.
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of special interest. Optical recording was used to study the activity of inner retinal neurons in immature ferrets. Confirming an earlier report [31], retinal ganglion cells were found to be activated synchronously in a wave-like pattern. In addition, amacrine cells were active at the same time. One possibility is that the amacrine cells drive the spontaneous firing of the ganglion cells.
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Wong RO, Chernjavsky A, Smith SJ, Shatz CJ. Early functional neural networks in the developing retina. of special interest Nature. 374:1995;716-718 Optical recording was used to study the activity of inner retinal neurons in immature ferrets. Confirming an earlier report [31], retinal ganglion cells were found to be activated synchronously in a wave-like pattern. In addition, amacrine cells were active at the same time. One possibility is that the amacrine cells drive the spontaneous firing of the ganglion cells.
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Nature
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Wong, R.O.1
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0028801041
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of special interest. Used mutli-electrode recording from ganglion cells to deduce the existence of a functionally significant pathway from rods to ganglion cells through rod - cone gap junctions. In principle, this study could have been done with single-electrode recording, but the number of experiments required would have been huge.
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DeVries SH, Baylor DA. An alternative pathway for signal flow from rod photoreceptors to ganglion cells in mammalian retina. of special interest Proc Natl Acad Sci USA. 92:1995;10658-10662 Used mutli-electrode recording from ganglion cells to deduce the existence of a functionally significant pathway from rods to ganglion cells through rod - cone gap junctions. In principle, this study could have been done with single-electrode recording, but the number of experiments required would have been huge.
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of outstanding interest. Describes simultaneous recording of many retinal ganglion cells in the salamander retina. Many of them fire synchronously. Evidence is presented that concerted spikes encode information not carried by individual cells. The authors propose that they represent symbols in a multi-neuronal code for vision.
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Meister M, Lagnado L, Baylor DA. Concerted signaling by retinal ganglion cells. of outstanding interest Science. 270:1995;1207-1210 Describes simultaneous recording of many retinal ganglion cells in the salamander retina. Many of them fire synchronously. Evidence is presented that concerted spikes encode information not carried by individual cells. The authors propose that they represent symbols in a multi-neuronal code for vision.
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Science
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Meister, M.1
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of outstanding interest. A review and extension of the work presented in [27] and [39]. The concept of a multi-neuronal code is further developed, including a formal analysis of the information capacity of the optic nerve under different assumptions about its codes.
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Meister M. Multineuronal codes in retinal signaling. of outstanding interest Proc Natl Acad Sci USA. 93:1996;609-614 A review and extension of the work presented in [27] and [39]. The concept of a multi-neuronal code is further developed, including a formal analysis of the information capacity of the optic nerve under different assumptions about its codes.
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Proc Natl Acad Sci USA
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of outstanding interest. The populations of beta (X) and alpha (Y) ganglion cells in the cat scale across the retina: their dendritic spread and receptive field width expand and their peak sensitivity declines. In this paper, the distribution and density of bipolar synapses upon them were used to predict the cells' electrophysiological sensitivity profiles. This examplifies Sterling's use of quantitative electron microscopy to illuminate physiological issues. See [47] for a good introduction to this method.
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Kier CK, Buchsbaum G, Sterling P. How retinal microcircuits scale for ganglion cells of different size. of outstanding interest J Neurosci. 15:1995;7673-7683 The populations of beta (X) and alpha (Y) ganglion cells in the cat scale across the retina: their dendritic spread and receptive field width expand and their peak sensitivity declines. In this paper, the distribution and density of bipolar synapses upon them were used to predict the cells' electrophysiological sensitivity profiles. This examplifies Sterling's use of quantitative electron microscopy to illuminate physiological issues. See [47] for a good introduction to this method.
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