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Volumn 271, Issue 5249, 1996, Pages 656-659

Horizontal cells of the primate retina: Cone specificity without spectral opponency

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL TYPE; COLOR VISION; INTERNEURON; MACACA; NONHUMAN; PHOTORECEPTOR; PRIORITY JOURNAL; RETINA CONE; RETINA GANGLION CELL; RETINA HORIZONTAL NERVE CELL;

EID: 0030065715     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5249.656     Document Type: Article
Times cited : (206)

References (32)
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    • R. L. De Valois, C J Smith, S T Kitai, A. J. Karoly, Science 127, 238 (1958); R. L. De Valois, G. H. Jacobs, I. Abramov, ibid 146, 1184 (1964); T N Wiesel and D. H. Hubel, J. Neurophysiol. 29, 1115 (1966), P. Gouras, J. Physiol (London) 199, 533 (1968)
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    • De Valois, R.L.1    Smith, C.J.2    Kitai, S.T.3    Karoly, A.J.4
  • 4
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    • R. L. De Valois, C J Smith, S T Kitai, A. J. Karoly, Science 127, 238 (1958); R. L. De Valois, G. H. Jacobs, I. Abramov, ibid 146, 1184 (1964); T N Wiesel and D. H. Hubel, J. Neurophysiol. 29, 1115 (1966), P. Gouras, J. Physiol (London) 199, 533 (1968)
    • (1964) Science , vol.146 , pp. 1184
    • De Valois, R.L.1    Jacobs, G.H.2    Abramov, I.3
  • 5
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    • R. L. De Valois, C J Smith, S T Kitai, A. J. Karoly, Science 127, 238 (1958); R. L. De Valois, G. H. Jacobs, I. Abramov, ibid 146, 1184 (1964); T N Wiesel and D. H. Hubel, J. Neurophysiol. 29, 1115 (1966), P. Gouras, J. Physiol (London) 199, 533 (1968)
    • (1966) J. Neurophysiol. , vol.29 , pp. 1115
    • Wiesel, T.N.1    Hubel, D.H.2
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    • R. L. De Valois, C J Smith, S T Kitai, A. J. Karoly, Science 127, 238 (1958); R. L. De Valois, G. H. Jacobs, I. Abramov, ibid 146, 1184 (1964); T N Wiesel and D. H. Hubel, J. Neurophysiol. 29, 1115 (1966), P. Gouras, J. Physiol (London) 199, 533 (1968)
    • (1968) J. Physiol (London) , vol.199 , pp. 533
    • Gouras, P.1
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    • note
    • Eyes from macaque (Macaca nemestnna, Macaca fascicularis, or Macaca mulatta) were enucleated after euthanasia The retina, together with the pigment epithelium and choroid layer, was dissected and mounted in a superfusion chamber on the stage of a light microscope. Temperature was maintained in the chamber at 36° ± 1°C and superfused at ∼5 ml/min. Vital labeling of horizontal cell nuclei was achieved by incubating the eye cup before retinal dissection for ∼20 min in Ames medium to which diamidinophenylindole (DAPI) was added at a concentration of ∼10 μM. Intracellular penetrations were made under direct visual control with high-resistance micropipettes, and light responses were recorded in conventional bridge mode. The recorded cells were directly observed during recording by iontophoretic injection of the fluorescent dye pyranine. At the termination of recording, cells were preserved for later analysis by injection of Neurobiotin and subsequent horseradish peroxidase histochemistry after tissue fixation (70).
  • 19
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    • note
    • Output of the LEDs was modulated by a circuit driver under computer control (23) A spectroradiometric calibration was performed in the plane of the retina with a Gamma Scientific spectral radiometer. Radiometnc measures were converted to photometric units (illuminance) by convolution with the human photopic spectral luminosity function (24). We estimated retinal illuminance in these experiments to be ∼1000 trolands.
  • 21
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    • note
    • min correspond to the quantal absorptions calculated from the cone fundamentals. We confirmed S-cone isolation by checking for a null response in M-parasol ganglion cells, which were previously shown to lack S-cone input (26)
  • 22
    • 2242428085 scopus 로고
    • No other horizontal cell response properties or distinctive dendritic morphologies were found, despite our large sample of recorded cells All of the morphological features attributed to a third (H3) horizontal cell type (9) were encompassed in our sample of H1 cells; H3 cells appear to reflect normal variation in the H1 cell population, with no separate physiological role. Our picture of the H1 and H2 cell mosaic is consistent with previous evidence that single cells can either avoid or "seek out" S-cones (9). This conclusion was recently confirmed by combining intracellular labeling of the horizontal cells and staining of the S-cone pedicles with an antibody to S-cone opsin [A. C. Goodchild, T. L. Chan, U Grunert, Soc. Neurosci Abstr. 21, 1645 (1995)].
    • (1995) Soc. Neurosci Abstr. , vol.21 , pp. 1645
    • Goodchild, A.C.1    Chan, T.L.2    Grunert, U.3
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    • R. C. Reid and R. M. Shapley, Nature 356, 716 (1992); P. Lennie, P W Haake, D. R Williams, in Computational Models of Visual Processing, M. S. Landy and J. A. Movshon, Eds. (MIT Press, Cambridge, MA, 1991), pp. 71-82
    • (1992) Nature , vol.356 , pp. 716
    • Reid, R.C.1    Shapley, R.M.2
  • 24
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    • M. S. Landy and J. A. Movshon, Eds. MIT Press, Cambridge, MA
    • R. C. Reid and R. M. Shapley, Nature 356, 716 (1992); P. Lennie, P W Haake, D. R Williams, in Computational Models of Visual Processing, M. S. Landy and J. A. Movshon, Eds. (MIT Press, Cambridge, MA, 1991), pp. 71-82
    • (1991) Computational Models of Visual Processing , pp. 71-82
    • Lennie, P.1    Haake, P.W.2    Williams, D.R.3
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    • (1995) Color Vision Deficiencies XII , pp. 177-184
    • Lee, B.B.1    Yen, T.2
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    • note
    • We thank the Regional Primate Center at the University of Washington for pnmate tissue; S Buck, K. Bora, and P Boro for their contributions; and B. Boycott, H. Wassle, H. Sherk, K. Mulligan, and M. Crognale for comments.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.