Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep

FEMS Microbiology Letters

Volumn 140, Issue 2-3, 1996, Pages 139-144

  Cloeckaert, Axel ^a   Debbarh, Hanane Salih Alj ^a   Vizcaíno, Nieves ^a   Saman, Eric ^b   Dubray, Gérard ^a   Zygmunt, Michel S ^a  

^a CEMAGREF   (France)

^b INNOGENETICS   (Belgium)

Author keywords

BP26; Brucella abortus; Brucella melitensis; CP28

Indexed keywords

BRUCELLA VACCINE; PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLOSIS; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOGENICITY; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SHEEP;

BRUCELLA MELITENSIS;

EID: 0030200424     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1097(96)00169-3     Document Type: Article

References (17)

1. [1] Alton, G.G. (1990) Brucella melitensis. In: Animal Brucellosis (Nielsen, K. and Duncan, J.R.. Eds.), pp. 384-409. CRC Press, Boca Raton, FL.
2. [2] Jiménez de Bagüés, M.P., Marin, C.M., Blasco. J.M., Moriyon, I. and Gamazo, C. (1992) An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev.1 vaccination. Vet. Microbiol. 30. 233-241.
3. [3] Dubray, G (1985) Antigens of diagnosis significance in Brucella. In: Brucella melitensis (Verger, J.M. and Plommet, M., Eds), pp. 123-138. Martinus Nijhoff, Dordrecht.
4. [4] Debbarh, H.S.A., Cloeckaert, A.. Zygmunt, M.S. and Dubray, G. (1995) Identification of sero-reactive Brucella melitensis cytosoluble proteins which discriminate between antibodies elicited by infection and Rev.1 vaccination in sheep. Vet. Microbiol. 44, 37-48.
5. [5] Salih-Alj Debbarh, H., Cloeckaert, A., Bézard, G.. Dubray, G. and Zygmunt, M.S. (1996) Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev. 1-vaccinated sheep. Clin. Diagn. Lab. Immunol. 3 (in press).
6. [6] Alton, G.G., Jones. L.M., Angus. D.D. and Verger, J.M. (1988) Techniques for the Brucellosis Laboratory. Institut National de la Recherche Agronomique, Paris.
7. [7] Cloeckaert, A., Salih-Alj Debbarh, H., Zygmunt, M.S. and Dubray, G. (1996) Production and characterization of monoclonal antibodies to Brucella melitensis cytosoluble proteins which are able to differentiate antibody responses of infected from Rev.1 vaccinated sheep. J. Med. Microbiol. (in press).
8. [8] Verger, J.M., Grimont, F.. Grimont, P.A.D. and Grayon. M. (1985) Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 35, 292-295.
9. [9] Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10. [10] Sanger, F., Nickten, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
11. [11] Rossetti, O.L., Arese, A.I., Boschiroli, M.L. and Cravero, S.L. ( 1996) Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis. J. Clin. Microbiol. 34, 165-169.
12. [12] Sangari, F.J., Garcia-Lobo, J.M. and Agüero, J. (1994) The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 121, 337-342.
13. [13] Albertini. A.M., Hofer, M., Calos, M.P. and Miller, J.H. (1982) On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions. Cell 29, 319-328.
14. [14] Allgood, N.D. and Silhavy, T.J. (1988) Illegitimate recombination in bacteria. In: Genetic Recombination (Kucherlapati, R. and Smith, G.R., Eds.), pp. 309-330. ASM Press, Washington, DC.
15. [15] Singer, B.S. and Westlye, J. (1988) Deletion formation in bacteriophage T4. J. Mol. Biol. 202, 233-243.
16. [16] Cloeckaert, A., Verger, J.-M.. Grayon, M. and Grépinet, O. (1995) Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer membrane proteins of Brucella. Microbiology 141, 2111-2121.
17. [17] Cloeckaert, A., Verger, J.-M., Grayon. M., Zygmunt, M.S. and Grépinet, O. (1996) Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene. Infect. Immun. 64 (in press).