Nucleotide sequence and expression of the gene encoding the major 25-kilodalton outer membrane protein of Brucella ovis: Evidence for antigenic shift, compared with other Brucella species, due to a deletion in the gene

Infection and Immunity

Volumn 64, Issue 6, 1996, Pages 2047-2055

  Cloeckaert, Axel ^a   Verger, Jean Michel ^a   Grayon, Maggy ^a   Zygmunt, Michel S ^a   Grépinet, Olivier ^a  

^a CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

OUTER MEMBRANE PROTEIN;

ANTIGENIC MODULATION; ARTICLE; BACTERIAL GENE; BRUCELLA; BRUCELLA ABORTUS; BRUCELLA CANIS; BRUCELLA MELITENSIS; BRUCELLA SUIS; CATTLE DISEASE; GENE DELETION; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SPECIES DIFFERENCE; VACCINE PRODUCTION;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BACTERIAL OUTER MEMBRANE PROTEINS; BASE SEQUENCE; BRUCELLA; ESCHERICHIA COLI; GENE DELETION; GENES, BACTERIAL; MICROSCOPY, IMMUNOELECTRON; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT;

EID: 0029883121     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.6.2047-2055.1996     Document Type: Article

References (39)

1. Albertini, A. M., M. Hofer, M. P. Calos, and J. H. Miller. 1982. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions. Cell 29:319-328.
2. Allgood, N. D., and T. J. Silhavy. 1988. Illegitimate recombination in bacteria, p. 309-330. In R. Kucherlapati and G. R. Smith (ed.), Genetic recombination. American Society for Microbiology, Washington, D.C.
3. Alton, G. G., L. M. Jones, R. D. Angus, and J. M. Verger. 1988. Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris.
4. Blasco, J. M. 1990. Brucella ovis, p. 351-378. In K. Nielsen and J. R. Duncan (ed.), Animal brucellosis. CRC Press, Inc., Boca Raton, Fla.
5. Bowden, R. A., A. Cloeckaert, M. S. Zygmunt, S. Bernard, and G. Dubray. 1995. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. Infect. Immun. 63:3945-3952.
6. Brunham, R. C., F. A. Plummer, and R. S. Stephens. 1993. Bacterial antigenic variation, host immune response, and pathogen-host coevolution. Infect. Immun. 61:2273-2276.
7. Cloeckaert, A., P. de Wergifosse, G. Dubray, and J. N. Limet. 1990. Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. Infect. Immun. 58:3980-3987.
8. Cloeckaert, A., I. Jacques, N. Bosseray, J. N. Limet, R. Bowden, G. Dubray, and M. Plummet. 1991. Protection conferred on mice by monoclonal antibodies directed against outer-membrane-protein antigens of Brucella. J. Med. Microbiol. 34:175-180.
9. Cloeckaert, A., P. Kerkhofs, and J. N. Limet. 1992. Antibody response to Brucella outer membrane proteins in bovine brucellosis: immunoblot analysis and competitive enzyme-linked immunosorbent assay using monoclonal antibodies. J. Clin. Microbiol. 30:3168-3174.
10. Cloeckaert, A., J.-M. Verger, M. Grayon, and O. Grépinet. 1995. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer membrane proteins of Brucella. Microbiology 141:2111-2121.
11. Cloeckaert, A., M. S. Zygmunt, G. Bezard, and G. Dubray. 1996. Purification and antigenic analysis of the major 25-kilodalton outer membrane protein of Brucella abortus. Res. Microbiol. 147:225-235.
12. Cloeckaert, A., M. S. Zygmunt, P. de Wergifosse, G. Dubray, and J. N. Limet. 1992. Demonstration of peptidoglycan-associated Brucella outer membrane proteins by use of monoclonal antibodies. J. Gen. Microbiol. 138:1543-1550.
13. Cloeckaert, A., M. S. Zygmunt, J.-C. Nicolle, G. Dubray, and J. N. Limet. 1992. O-chain expression in the rough Brucella melitensis strain B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy. J. Gen. Microbiol. 138:1211-1219.
14. AL, p. 377-388. In N. R. Krieg and J. G. Holt (ed.), Bergey's manual of systematic bacteriology, vol. I. The Williams & Wilkins Co., Baltimore.
15. de Wergifosse, P., P. Lintermans, J. N. Limet, and A. Cloeckaert. 1995. Cloning and nucleotide sequence of the gene coding for the major 25-kilodalton outer membrane protein of Brucella abortus. J. Bacteriol. 177: 1911-1914.
16. Douglas, J. T., E. Y. Rosenberg, H. Nikaido, D. R. Verstreate, and A. J. Winter. 1984. Porins of Brucella species. Infect. Immun. 44:16-21.
17. Dubray, G. 1987. Protective antigens in brucellosis. Ann. Inst. Pasteur Microbiol. 138:84-86.
18. Dubray, G., and G. Bézard. 1980. Isolation of three Brucella abonus cell-wall antigens protective in murine experimental brucellosis. Ann. Rech. Vet. 11: 367-373.
19. Dubray, G., and C. Charriaut. 1983. Evidence of three major polypeptide species and two major polysaccharide species in the Brucella outer membrane. Ann. Rech. Vet. 14:311-318.
20. Ficht, T. A., S. W. Bearden, B. A. Sowa, and H. Marquis. 1990. Genetic variation at the omp2 porin locus of the brucellae: species-specific markers. Mol. Microbiol. 4:1135-1142.
21. Gamazo, C., A. I. Vitas, I. Moriyon, I. Lopez-Goni, and R. Diaz. 1993. Brucella group 3 outer membrane proteins contain a heat-modifiable protein. FEMS Microbiol. Lett. 112:141-146.
22. Gamazo, C., A. J. Winter, I. Moriyon, J. I. Riezu-Boj, J. M. Blasco, and R. Diaz. 1989. Comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of Brucella ovis and Brucella melitensis. Infect. Immun. 57:1419-1426.
23. Glickman, B. W., and L. S. Ripley. 1984. Structural intermediates of deletion mutagenesis: a role for palindromic DNA. Proc. Natl. Acad. Sci. USA 81: 512-516.
24. Greenspan, N. S., and L. J. N. Cooper. 1992. Intermolecular cooperativity: a clue to why mice have IgG3? Immunol. Today 13:164-168.
25. Halling, S. M., F. M. Tatum, and B. J. Bricker. 1993. Sequence and characterization of an insertion sequence, IS711, from Brucella ovis. Gene 133: 123-137.
26. Jiménez de Bagüés, M. P., P. H. Elzer, J. M. Blasco, C. M. Marin, C. Gamazo, and A. J. Winter. 1994. Protective immunity to Brucella ovis in BALB/c mice following recovery from primary infection or immunization with subcellular vaccines. Infect. Immun. 62:632-638.
27. Kittelberger, R., F. Hilbink, M. F. Hansen, G. P. Ross, G. W. de Lisle, A. Cloeckaert, and J. de Bruyn. 1995. Identification and characterization of immunodominant antigens during the course of infection with Brucella ovis. J. Vet. Diagn. Invest. 7:210-218.
28. Ouahrani, S., S. Michaux, J. Sri Widada, G. Bourg, R. Tournebize, M. Ramuz, and J.-P. Liautard. 1993. Identification and sequence analysis of IS6501, an insertion sequence in Brucella spp.: relationship between genomic structure and the number of IS6501 copies. J. Gen. Microbiol. 139:3265-3273.
29. Riezu-Boj, J. I., I. Moriyon, J. M. Blasco, C. Gamazo, and R. Diaz. 1990. Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. Infect. Immun. 58:489-494.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sangari, F. J., J. M. Garcia-Lobo, and J. Agüero. 1994. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol. Lett. 121:337-342.
32. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Seifert, H. S., and M. So. 1988. Genetic mechanisms of bacterial antigenic variation. Microbiol. Rev. 52:327-336.
34. Singer, B. S., and J. Westlye. 1988. Deletion formation in bacteriophage T4. J. Mol. Biol. 202:233-243.
35. Sowa, B. A., K. A. Kelly, T. A. Ficht, M. Frey, and L. G. Adams. 1991. SDS-soluble and peptidoglycan-bound proteins in the outer membrane-peptidoglycan complex of Brucella abortus. Vet. Microbiol. 27:351-369.
36. Verger, J.-M., F. Grimont, P. A. D. Grimont, and M. Crayon. 1985. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. Int. J. Syst. Bacteriol. 35:292-295.
37. Verstreate, D. R., M. T. Creasy, N. T. Caveney, C. L. Baldwin, M. W. Blab, and A. J. Winter. 1982. Outer membrane proteins of Brucella abortus: isolation and characterization. Infect. Immun. 35:979-989.
38. Winter, A. J., G. E. Rowe, J. R. Duncan, M. J. Eis, J. Widom, B. Ganem, and B. Morein. 1988. Effectiveness of natural and synthetic complexes of porin and O-polysaccharide as vaccines against Brucella abortus in mice. Infect. Immun. 56:2808-2817.
39. Zygmunt, M. S., A. Cloeckaert, and G. Dubray. 1994. Brucella melitensis cell envelope protein and lipopolysaccharide epitopes involved in humoral immune responses of naturally and experimentally infected sheep. J. Clin. Microbiol. 32:2514-2522.