Biotechnology and biomimetic with crystalline bacterial cell surface layers (S-layers)

Micron

Volumn 27, Issue 2, 1996, Pages 141-156

  Sára, Margit ^a   Sleytr, Uwe B ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

Author keywords

Bacterial cell surface layers; biomimetic membranes; diagnostics; immobilization; liposomes; S layers; ultrafiltration membranes

Indexed keywords

BACTERIA (MICROORGANISMS); GEOBACILLUS STEAROTHERMOPHILUS; METHANOBACTERIA;

EID: 0030114743     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/0968-4328(96)80628-7     Document Type: Review

References (113)

1. Baumeister, W. and Engelhardt, H., 1987. Three-dimensional structure of bacterial surface layers. In: Electron Microscopy of Proteins, Vol. 6. Membraneous Structures, Harris, J.R. and Horne, R.W. (eds). Academic Press, New York, pp. 109-154.
2. Baumeister, W. and Lembcke, G., 1992. Structural features of archaeobacteria cell envelopes. J. Bioenerg. Biomemb. 24, 567-576.
3. Baumeister, W., Wildhaber, I. and Phipps, B. M., 1989. Principles of organization in eubacterial and archaeobacterial surface proteins. Can. J. Microbiol. 35, 215-227.
4. Beveridge, T. J., 1981. Ultrastructure chemistry and function of the bacterial cell wall. Int. Rev. Cytol. 72, 229-317.
5. Beveridge, T. J., 1994. Bacterial S-layers, Curr. Op. Struct. Biol 4, 204-212.
6. Beveridge, T. J. and Graham, L. L., 1991. Surface layers of bacteria. Microbiol. Rev. 51, 684-705.
7. Beveridge, T. J. and Koval, S. F. (eds) 1993. Advances in Bacterial Paracrystalline Surface Layers, Plenum Press, New York.
8. Blaser, M. J., 1993. Role of the S-layer proteins in Campylobacter fetus in serum-resistance and antigenic variation: a model of bacterial pathogenesis. Am: J. Med. Sci. 40, 269-285.
9. Bock, K., Schuster-Kolbe, J., Altman, E., Allmaier, G., Stahl, B., Christian, R., Sleytr, U. B. and Messner, P., 1994. Primary structure of the O-glycosidically linked glycan chain of the crystalline surface layer glycoprotein of Thermoanaerobacter thermohydrosulfuricus L111-69. J. Biol. Chem. 269, 7137-7144.
10. Boot, H. J., Kolen, C. P. A. M., van Noort, J. M. and Pouwels, P. H., 1993. S-layer protein of Lactobacillus acidophilus ATCC 4256: purification, expression in Escherichia coli and nucleotide sequence of the corresponding gene, J. Bacteriol. 175, 6089-6096.
11. Breitwieser, A., Gruber, K. and Sleytr, U. B., 1992. Evidence for an S-layer protein pool in the peptidoglycan of Bacillus stearothermophilus. J. Bacteriol. 174, 8008-8015.
12. Breitwieser, A., Küpcü, S., Howorka, S., Weigert, S., Langer, C., Hoffmann-Sommergruber, K., Scheiner O., Sleytr, U.B. and Sára, M., 1996. 2-D protein crystals as immobilization matrix and reaction zone for dipstick-style immunoassays. Biotechniques (in press).
13. Carl, M. and Dasch, G. A., 1989. The importance of the crystalline surface layer protein antigens of rickesttisa in T-cell immunity. J. Autoimmun. 2, 81-91.
14. Carl, M., Ching, W.-M. and Dasch, G. A., 1988. Recognition of typhus groups rickettsia infected targets by human lymphokine-activated killer cells. Infect. Immun. 56, 2526-2529.
15. Dworkin, J., Tummuru, M. K. R. and Blaser, M. J., 1995. A lipopolysaccharide binding domain of Campylobacter fetus S-layer protein resides within the conserved N-terminus of a family of silent and divergent genes. J. Bacteriol. 177, 1734-1741.
16. Egelseer, E., Schocher, I., Sára, M. and Sleytr, U. B., 1995. The S-layer from Bacillus stearothermophilus DSM 2358 functions as an adhesion site specific for a high-molecular weight amylase. J. Bacteriol. 174, 1444-1451.
17. Fane, A .G. and Fell, C. J. D., 1987. A review of fouling and fouling control in ultrafiltration. Desal. 62, 117-136.
18. Fujino, T., Beguin, P. and Aubert, J.-P., 1993. Organization of a Clostridium thermocellum gene cluster encoding the cellulosome scaffolding protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface. J. Bacteriol. 175, 1891-1899.
19. Garduno, R. A., Phipps, B. M. and Kay, W. W., 1994. Physiological consequences of the S-layer of Aeromonas salmonicida in relation to growth, temperature, and outer membrane separation. Can. J. Microbiol. 40, 622-629.
20. Gilchrist, A., Fischer, J. A. amd Smit, J., 1992. A nucleotide sequence analysis of the gene encoding the Caulobacter crescentus paracrystalline surface layer protein. Can. J. Microbiol. 38, 193-202.
21. Hastie, A. T. and Brinton, C. C., 1979. Specific interaction of the treagonally arrayed protein layer of Bacillus sphaericus with the peptidoglycan sacculus. J. Bacteriol. 138, 1010-1021.
22. Herak, D. C. and Merill, E. W., 1990. Affinity cross flow filtration: some new aspects. Biotechnol. Prog. 6, 33-40.
23. Hjelm, H., Sjödhal, J. and Sjöquist, ?., 1975. Immunological activity and structural similar fragments of protein A from Staphylococcus aureus. Eur. J. Biochem. 57, 395-403.
24. Hovmöller, S., 1993. Crystallographic image processing applications for S-layers. In: Advances in Paracrystalline Bacterial Surface Layers, Beveridge, T. and Koval, S.F. (eds) Plenum Press, New York, pp. 13-21.
25. Hovmöller, S., Sjögren, A. and Wang, D. N., 1988. The structure of crystalline bacterial surface layers. Prog. Biophys. Mol. Biol. 51, 131-163.
26. Kandler, O. and König, H., 1985. Cell envelopes of archaebacteria. In: The Bacteria, Arachaebacteria. Vol. VIII., Woese, C.R. and Wolfe, R.S. (eds). Academic Press, New York, pp. 413-457.
27. Kandler, O. and König, H., 1993. Cell envelopes of archaea: structure and chemistry. In: The Biochemistry of Archaea, Archaebacteria, Kates, N., Kushner, D.J. and Matheson, AT. (eds). Elsevier, Amsterdam, pp. 223-259.
28. Kay, W. W., Thornton, J. C. and Garduno, R. A., 1993. Structure-function aspects of the Aeromonas salmonicida S-layer. In: Advances in Bacterial Paracrystalline Surface Layers, Beveridge, T. J. and Koval, S.F. (eds). Plenum Press, New York, pp. 151-157.
29. Kim, M., Saito, K., Furusaki, S., Sugo, T. and Ishigaki, I., 1991. Protein adsorption capacity of a porous phenylalanine-containing membrane based on a polyethylene matrix. J. Chromatogr. 586, 27-33.
30. Kim, K. J., Fane, A. G. and Fell, C. J. C., 1992. Fouling mechanisms of membranes during protein ultrafiltration. J. Membrane Sci. 68, 79-91.
31. Kim, K. J., Chen, V. and Fane, A. G., 1993. Some factors determining protein aggregation during ultrafiltration. Biotechnol. Bioeng. 42, 260-265.
32. Kist, M. L. and Murray, R. G. E., 1984. Components of the regular surface array of Aquaspirillum serpens MW5 and their assembly in vitro. J. Bacteriol. 157, 599-606.
33. König, H., 1988. Archaebacterial cell envelopes, Can. J. Microbiol. 34, 395-406.
34. König, H. and Stetter, K. O., 1986. Studies on arachaebacterial S-layers. System. Appl. Microbiol. 7, 300-309.
35. Koval, S. F., 1988. Paracrystalline protein surface arrays on bacteria. Can. J. Microbiol. 34, 407-414.
36. Kuen, B. and Lubitz, W., 1996. Analysis of S-layer proteins and genes. In: Crystalline Bacterial Cell Surface Proteins, Sleytr, U.B., Messner, P., Pum, D. and Sára, M. (eds). Landes Company, Austin, TX, pp. 77-102.
37. Kuen, B., Sára, M. and Lubitz, W., 1996. Heterologous expression and self-assembly of the S-layer protein SbsA of Bacillus stearothermophilus in Escherichia coli. Mol. Microbiol. 19, 495-503.
38. Kuen, B., Sleytr, U. B. and Lubitz, W., 1994. Sequence analysis of the sbsA gene encoding the 130-kD surface-layer protein of Bacillus stearothermophilus strain PV72. Gene 145, 115-120.
39. Küpcü, S., Sára, M. and Sleytr, U. B., 1991. Chemical modification of crystalline ultrafiltration membranes and immobilization of macromolecules. J. Membrane Sci. 61, 165-175.
40. Küpcü, S., Sára, M. and Sleytr, U. B., 1993. Influence of covalent attachment of low molecular weight substances on the rejection and adsorption characteristics of crystalline proteinaceous ultrafiltration membranes. Desal. 90, 65-76.
41. Küpcü, S., Mader, C. and Sára, M., 1995. The crystalline cell surface layer from Thermoanaerobacter thermohydrosulfuricus L111-69 as an immobilization matrix: influence of the morphological properties and the pore size of the matrix on loss of activity of covalently bound enzymes. Biotechnol. Appl. Biochem. 21, 275-286.
42. Küpcü, S., Sára, M. and Sleytr, U. B., 1995. Liposomes coated with crystalline bacterial cell surface proteins (S-layer) as immobilization structure for macromolecules. Biochim. Biophys. Acta 1235, 263-269.
43. Küpcü, S., Sleytr, U. B. and Sára, M., 1996. Two-dimensional protein crystals (S-layers) as novel matrix for immobilization of IgG and applicability of S-layers as microparticles in immunoassays. J. Immunol. Meth., (in press).
44. Langone, J. J., 1982. Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by Streptococci and Pneumococci. Adv. Immunol. 32, 157-252.
45. Lemaire, M., Ohayon, H., Gounon, P. and Beguin, P., 1995. OlpB, a new outer layer protein of Clostridium thermocellum and binding of its S-layer like domains to components of the cell envelope. J. Bacteriol. 177, 2451-2459.
46. Luong, J. H. T., Male, K. B. and Nguyen, A. L., 1988. A continuous affinity ultrafiltration process for trypsin purification. Biotechnol. Bioeng. 31, 516-520.
47. Luong, J. H. T., Male, K. B. and Nguyen, A. L., 1988. Mathematical modelling of affinity ultrafiltration processes. Biotechnol. Bioeng. 32, 451-459.
48. Malcolm, A.J., Best, M.W., Szarka, R.J., Mosleh, Z., Unger, F.M., Messner, P. and Sleytr, U.B., 1993. Surface layers from Bacillus alvei as a carrier for a Streptococcus pneumoniae conjugate vaccine. In: Advances in Paracrystalline Bacterial Surface Layers, Beveridge, T.J. and Koval, S.F. (eds). Plenum Press, New York, pp. 219-233.
49. Masuda, K. and Kawata, T., 1981. Characterization of a regular array in the wall of Lactobacillus buchneri and its reattachment to other wall components. J. Gen. Microbiol. 134, 81-90.
50. Matuschek, M., Burchhardt, G., Sahm, K. and Bahl, H., 1994. Pullulanase of Thermoanaerobacterium thermosulfurigenes EM1, Clostridium thermosulfurogenes: molecule analysis of the gene, composite structure of the enzyme, and a common model for its attachment to the cell surface. J. Bacteriol. 176, 3295-3303.
51. Messner, P., 1996. Chemical composition and biosynthesis of S-layers. In: Crystalline Bacterial Cell Surface Proteins, Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds). Landes Company, Austin, TX, pp. 35-76.
52. Messner, P. and Sleytr, U. B., 1991. Bacterial surface layer glycoproteins. Glycobiology 1, 545-551.
53. Messner, P. and Sleytr, U. B., 1992. Crystalline bacterial cell-surface layers. Adv. Microbiol. Physiol. 33, 213-275.
54. Messner, P., Mazid, M. A., Unger, F. M. and Sleytr, U. B., 1992. Artifical antigens. Synthetic carbohydrate haptens immobilized on crystalline bacterial surface layer glycoproteins. Carbohydr. Res. 233, 175-184.
55. Messner, P., Unger, F. M. and Sleytr, U. B., 1996. Vaccine development based on S-layer technology. In: Crystalline bacterial cell surface proteins, Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds). Landes Company, Austin, TX, pp. 161-173.
56. Munn, C. B., Ishiguro, E. E., Kay, W. W. and Trust, T. J., 1982. Role of surface components in serum resistance of virulent Aeromonas salmonicida. Infect. Immun. 36, 3816-3825.
57. Neubauer, A., Pum, D. and Sleytr, U. B., 1993. An amperometric glucose sensor based on isoporous crystalline protein membranes as immobilization matrix. Anal. Lett. 26, 1347-1360.
58. Neubauer, A., Hödl, C., Pum, D. and Sleytr, U. B., 1994. A multistep enzyme sensor for sucrose based on S-layer microparticles as immobilization matrix. Anal. Lett. 27, 849-865.
59. Neubauer, A., Pum, D., Sleytr, U. B., Klimant, I. and Wolfbeis, O., 1996. Fibre-optic glucose sensor using enzyme membranes with 2-D crystalline structure. Biosensors Bioelectronics 113, 317-325.
60. Nilson, J. L., 1990. Protein fouling of ultrafiltration membranes: causes and consequences. J. Membr. Sci. 52, 121-142.
61. Pei, Z. and Blaser, M. J., 1990. Pathogenesis of Campylobacter infections. Role of surface array proteins in virulence in a mouse model. J. Clin. Invest. 85, 1036-1043.
62. Pei, Z., Ellison, R. T., Lewis, R. V. and Blaser, M. J., 1988. Purification and characterization of a family of high molecular weight surface-array proteins from Campylobacter fetus. J. Biol. Chem. 263, 6416-6420.
63. Phipps, B. and Kay, W. W., 1988. Immunoglobulin binding by the _regular surface array of Aeromonas salmonicida. J. Biol. Chem. 263, 9298-9303.
64. Pum, D. and Sleytr, U. B., 1994. Large-scale reconstruction of crystalline bacterial surface layer proteins at the air - water interface and on lipids. Thin Solid Films 244, 882-886.
65. Pum, D. and Sleytr, U.B., 1996. Molecular nanotechnology and biomimetics with S-layers. In: Crystalline Bacterial Cell Surface Proteins. Sleytr, U.B., Messner, P., Pum, D. and Sára, M. (eds) Landes Company, Austin, TX, pp. 175-209.
66. Pum, D., Sára, M. and Sleytr, U. B., 1989. Structure, surface charge and self-assembly of the S-layer lattice from Bacillus coagulans E38/66. J. Bacteriol. 171, 5296-5303.
67. Pum, D., Weinhandl, M., Hödl, C. and Sleytr, U. B., 1993. Large-scale recrystallization of the S-layer of Bacillus coagulans E38/66 at the air/water interface and on lipd films. J. Bacteriol. 175, 2762-2766.
68. Pum, D., Sára, M. and Sleytr, U. B., 1993. Two-dimensional (glyco)-protein crystals as patterning elements and immobilisation matrices for the development of biosensors. In: Immobilised Macromolecules. Application Potentials. Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds). Springer, London, pp. 143-160.
69. Sára, M. and Egelseer, E., 1996. Functional aspects of S-layers. In: Crystalline Bacterial Cell Surface Proteins, Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds) Landes Company, Austin, TX, pp. 103-131.
70. Sára, M. and Sleytr, U. B., 1987a. Molecular-sieving through S layers of Bacillus stearothermophilus strains. J. Bacteriol. 169, 4092-4098.
71. Sára, M. and Sleytr, U. B., 1987b. Production and characteristics of ultrafiltration membranes with uniform pores from two-dimensional arrays of proteins. J. Membr. Sc. 33, 27-49.
72. Sára, M. and Sleytr, U. B., 1987c. Charge distribution of the S-layer of Bacillus stearothermophilus NRS 1536/3c and importance of charged groups for morphogenesis and function. J. Bacteriol. 169, 2084-2089.
73. Sára, M. and Sleytr, U. B., 1988. Membrane biotechnology: Two-dimensional protein crystals for ultrafilration purposes. In: Biotechnology Vol. 6b. Rehm, H.-J. and Reed, G. (eds). VCH, Weinheim, pp. 615-636.
74. Sára, M. and Sleytr, U. B., 1989. Use of regularly structured bacterial cell surface layers as matrix for immobilizing macromolecules. Appl. Microbiol. Biotechnol. 30, 184-189.
75. Sára, M. and Sleytr, U. B., 1993. Relevance of charged groups for the integrity of the S-layer lattice from Bacillus coagulans E38-66 and for molecular interactions. J. Bacteriol. 175, 2248-2254.
76. Sára, M. and Sleytr, U. B., 1994. Comparative studies of S-layer proteins from Bacillus stearothermophilus strains expressed during growth in continuous culture under oxygen-limited and non oxygen-limited conditions. J. Bacteriol. 176, 848-860.
77. Sára, M., Küpcü, S. and Sleytr, U. B., 1989.Localization of the carbohydrate residue of the S-layer glycoprotein from Clostridium thermohydrosulfuricum L111-69. Arch. Microbiol. 151, 416-420.
78. Sára, M., Moser-Thier, K., Kainz, U. and Sleytr, U. B., 1990. Characterization of S-layers from mesophilic bacillaceae and studies on their protective role towards muramidases. Arch. Microbiol. 153, 209-214.
79. Sára, M., Pum, D. and Sleytr, U. B., 1992. Permeability and charge-dependent adsorption properties of the S-layer lattice from Bacillus coagulans E38-66. J. Bacteriol. 174, 3487-3493.
80. Sára, M., Küpcü, S., Weiner, C., Weigert, S. and Sleytr, U. B., 1993. Crystalline protein layers as isoporous molecular sieves and immobilisation matrices. In: Immobilised Macromolecules. Application potentials, Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds). Springer, London, pp. 71-86.
81. Sára, M., Kücpü, S. and Sleytr, U. B., 1996. Biotechnological applications of S-layers. In: Crystalline Bacterial Cell Surface Proteins, Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds). Landes Company, Austin, TX, pp. 133-159.
82. Sára, M., Kuen, B., Mayer, H., Mandl, F., Schuster, K. C. and Sleytr, U. B., 1996. Dynamcis in oxygen-induced changes in S-layer protein synthesis and cell wall composition in continuous culture from Bacillus stearothermophilus PV72 and its S-layer-deficient variant T5. J. Bacteriol. 178, 2108-2117.
83. Schneitz, C., Nuotio, L. and Lounatmaa, K., 1992. Adhesion of Lactobacillus acidophilus to avian intestinal epithelial cells mediated by the crystalline bacterial cell surface layer, S-layer. J. Appl. Bacteriol. 74, 290-294.
84. Schultze-Lam, S. and Beveridge, T. J., 1994. Nucleation of celestite and strontianite on a cyanobacterial S-layer. Appl. Environ. Microbiol. 60, 447-453.
85. Schultze-Lam, S., Harauz, G. and Beveridge, T. J., 1992. Participation of a cyanobacterial S-layer in fine grain mineral formation. J. Bacteriol. 174, 7971-7981.
86. Schuster, K. C., Mayer, H., Kieweg, R., Hampel, W. and Sára, M., 1995. A synthetic medium for continuous culture of the S-layer carrying Bacillus stearothermophilus PV72 and studies on the influence of growth conditions on cell wall properties. Biotechnol. Bioeng. 48, 66-77.
87. Sjöquist, J., Meloun, B. and Hjelm, H., 1972. Protein A isolated from Staphylococcus aureus after digestion with Lysostaphin. Eur. J. Biochem. 29, 572-578.
88. Sleytr, U. B., 1978. Regular arrays of macromolecules on bacterial cell walls: structure, chemistry, assembly and function. Int. Rev. Cytol. 53, 1-64.
89. Sleytr, U. B. and Glauert, A. M., 1976. Ultrastructure of the cell walls of two closely realted clostridia that possess different regular arrays of surface subunits. J. Bacteriol. 126, 869-882.
90. Sleytr, U. B. and Messner, P., 1983. Crystalline surface layers on bacteria. Annu. Rev. Microbiol. 37, 311-339.
91. Sleytr, U. B. and Messner, P., 1989. Self-assembly of crystalline bacterial cell surface layers, S-layers. In: Electron Microscopy of Subcellular Dynamics, Plattner, H. (ed.), CRC Press, Boca Raton, pp. 13-31.
92. Sleytr, U. B. and Sára, M., 1989a. Structure with membranes having continuous pores. United States of America Patent 4,886,604.
93. Sleytr, U. B. and Sára, M., 1989b. Use of a structure with membranes having continuous pores. United States of America Patent 4,849,109.
94. Sleytr, U. B. and Sára, M., 1995. Verwendung einer mit immobilisierten Molekülen bzw. Substanzen versehenen Membrane. Europäische Patentschrift, 0 362 339 B1.
95. Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds) 1988. Crystalline Bacterial Cell Surface Layers. Springer, Berlin.
96. Sleytr, U. B., Mundt, W., Messner, P., Smith, R. M. and Unger, F. M., 1991. Immunogenic composition containing ordered carriers. United States of America Patent 5,043,158.
97. Sleytr, U. B., Messner, P., Pum, D. and Sára, M., 1993. Crystalline bacterial cell surface layers. Mol. Microbiol. 10, 911-916.
98. Sleytr, U. B., Messner, P., Pum, D. and Sára, M. (eds), 1996. Crystalline bacterial cell surface proteins. Landes Company, Austin, TX.
99. Smit, J., 1986. Protein surface layers of bacteria. In: Bacterial Outer Membranes as Model Systems, Inouye, M. (ed). Wiley, New York, pp. 343-376.
100. Smith, R. H., Messner, P., Lamontagne, L. R., Sleytr, U. B. and Unger, F. M., 1993. Induction of T-cell immunity to oligosaccharide antigens immobilized on crystalline bacterial surface layers, S-layers. Vaccine 11, 919-924.
101. Strathmann, H., 1981. Membrane separation processes, J. J. Membr. Sci. 10, 121-143.
102. Sumper, M. and Wieland, F. T., 1995. Bacterial glycoproteins. In: Glycoproteins, Montreuil, J., Vliegenhardt, J. F. G. and Schachter, H. (eds). Elsevier, Amsterdam, pp. 455-473.
103. Takeoka, A., Takumi, K. and Kawata, T., 1991. Purification and characterization of S-layer proteins from Clostridium difficile GAI 0714. J. Gen. Microbiol. 137, 261-267.
104. Tsuboi, A., Tsukagoshi, N. and Udaka, S., 1982. Reassembly in vitro of hexagonal surface arrays in a protein-producing bacterium, Bacillus brevis 47. J. Bacteriol. 151, 14485-14497.
105. Tummuru, M. and Blaser, M. J., 1993. Rearrangement of sap A homologs with conserved and variable regions in Campylobacter fetus. Proc. Natl. Acad. Sci. USA 90, 7265-7269.
106. Vidgren, G., Palva, I., Pakkanen, R., Lounatmaa, K. and Palva, A., 1992. S-layer protein gene of Lactobacillus brevis: cloning by polymerase chain reaction and determination of the nucleotide sequence. J. Bacteriol. 174, 7419-7427.
107. Walker, S. G., Nedra-Karunaratne, D., Ravenscroft, N. and Smit, J., 1994. Characterization of mutants of Caulobacter crescentus defective in surface attachment to the paracrystalline surface layer protein. Can. J. Microbiol. 38, 193-202.
108. Wang, E., Garcia, M., Blake, M. S. and Blaser, M. J., 1993. Shift in S-layer protein expression responsible for antigenic variation in Campylobacter fetus. J. Bacteriol. 175, 4979-4984.
109. Weigert, S. and Sára, M., 1995. Surface modification of an ultrafiltration membrane with crystalline structure and studies on interactions with selected protein molecules. J. Membr. Sci. 106, 147-159.
110. Weigert, S. and Sára, M., 1996. Ultrafiltration membranes produced from 2-D protein crystals as model systems for studying the importance of the net charge, the charge density, the accessiblity of charged groups and the hydrophobicity of the active filtration layer for protein adsorption. J. Membr. Sci. (submitted).
111. Weiner, C., Sára, M. and Sleytr, U. B., 1994. Novel Protein A affinity matrix prepared from two-dimensional protein crystals. Biotechnol. Bioeng. 43, 321-330.
112. Weiner, C., Sára, M., Dasgupta, G. and Sleytr, U. B., 1994. Affinity cross-flow filtration: purification of IgG with a novel Protein A affinity matrix prepared from two-dimensional protein crystals. Biotechnol. Bioeng. 44, 55-65.
113. Yang, L., Pei, Z., Fujimoto, S. and Blaser, M. J., 1992. Reattachment of surface array proteins to Campylobacter fetus cells. J. Bacteriol. 174, 1258-1267.