IgM-producing chronic lymphocytic leukemia cells undergo immunoglobulin isotype-switching without acquiring somatic mutations

Journal of Clinical Investigation

Volumn 98, Issue 2, 1996, Pages 290-298

  Efremov, Dimitar G ^a,b   Ivanovski, Martin ^a,b   Batista, Facundo D ^a   Pozzato, Gabriele ^c   Burrone, Oscar R ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

^b SS CYRIL AND METHODIUS UNIVERSITY OF SKOPJE   (Macedonia)

^c UNIVERSITY OF TRIESTE   (Italy)

Author keywords

B cell leukemia; CD5 antigen; class switching; heavy chain; immunoglobulin variable region

Indexed keywords

CD5 ANTIGEN; DNA FRAGMENT; IMMUNOGLOBULIN CLASS; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN M; MESSENGER RNA; RNA;

ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; B LYMPHOCYTE DIFFERENTIATION; CELL SUBPOPULATION; CHRONIC LYMPHATIC LEUKEMIA; CLINICAL ARTICLE; GENE REARRANGEMENT; GENE SWITCHING; HUMAN; HUMAN CELL; IMMUNOGLOBULIN GENE; IMMUNOGLOBULIN PRODUCTION; LEUKEMIA CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

EID: 0030017254     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118792     Document Type: Article

References (42)

1. Foon, K.A., K.R. Rai, and R.P. Gale. 1990. Chronic lymphocytic leukemia: new insights into biology and therapy. Ann. Int. Med. 113:525-539.
2. Dighiero, G., P. Travade, S. Chevret, P. Fenaux, C. Chastang, J.-L. Binet, and the French Cooperative Group on CLL. 1991. B-cell chronic lymphocytic leukemia: Present status and future directions. Blood 78:1901-1914.
3. Geisler, C.H., J.K. Larsen, N.E. Hansen, M.M. Hansen, B.E. Christensen, B. Lund, H. Nielsen, T. Plesner, K. Thorling, E. Andersen, and P.K. Andersen. 1991. Prognostic importance of flow cytometric immunophenotyping of 540 consecutive patients with B-cell chronic lymphocytic leukemia. Blood. 78:1795-1802.
4. Friedman, D.F., J.S. Moore, J. Erikson, J. Manz, J. Goldman, P.C. Nowell, and L.E. Silberstein. 1992. Variable region gene analysis of an isotype-switched (IgA) variant of chronic lymphocytic leukemia. Blood. 80:2287-2297.
5. Fu, S.M., N. Chiorazzi, H.G. Kunkel, J.P. Halper, and S. Harris. 1978. Induction of in vitro differentiation and immunoglobulin synthesis of human leukemic B lymphocytes. J. Exp. Med. 148:1570-1578.
6. Totterman, T.H., N. Nilsson, and C. Sundstrom. 1980. Phorbol ester-induced differentiation of chronic lymphocytic leukemia cells. Nature (Lond.). 288:176-178.
7. Gordon, J., H. Mellstedt, P. Aman, P. Biberfeld, and G. Klein. 1984. Phenotypic modulation of chronic lymphocytic leukemia cells by phorbol ester: Induction of IgM secretion and changes in the expression of B cell-associated surface antigens. J. Immunol. 132:541-547.
8. Juliusson, G., K.-H. Rober, L. Hammarstrom, C.I.E. Smith, G. Biberfeld, and G. Gahrton. 1983. Mitogen-induced switching of immunoglobulin heavy-chain class secretion in chronic B-lymphocytic leukaemia and immunocytoma cell populations. Scand. J. Immunol. 17:51-59.
9. Sarfati, M., H. Luo, and G. Delespesse. 1989. IgE synthesis by chronic lymphocytic leukemia cells. J. Exp. Med. 170:1775-1780.
10. Fu, S.M., R.J. Winchester, T. Feizi, P.D. Walzer, and H.G. Kunkel. 1974. Idiotypic specificity of surface immunoglobulin and the maturation of leukemic bone-marrow-derived lymphocytes. Proc. Natl. Acad. Sci. USA 71:4487-4490.
11. Deegan, M.J., J.P. Abraham, M. Sawdyl, and E.J. van Slyck. 1984. High incidence of monoclonal proteins in the serum and urine of chronic lymphocytic leukemia patients. Blood. 64:1207-1211.
12. Sinclair, D., J.H. Dagg, A.E. Dewar, A.M. Mowat, D.M.V. Parrot, G. Stockdill, and D.I. Stott. 1986. The incidence, clonal origin and secretory nature of serum paraproteins in chronic lymphocytic leukemia. Br. J. Haematol. 64: 725-735.
13. Pezzoli, A., and E. Pascali. 1986. Monoclonal Bence Jones proteinuria in chronic lymphocytic leukemia. Scand J. Haematol. 36:18-24.
14. Qian, G.-X., S.M. Fu, D.L. Solanki, and K.R. Rai. 1984. Circulating monoclonal IgM proteins in B cell chronic lymphocytic leukemia: Their identification, characterization and relationship to membrane IgM. J. Immunol. 133: 3396-3400.
15. Hansen, D.A., B.A. Robbins, D.J. Bylund, L.D. Piro, A. Saven, and D.J. Ellison. 1994. Identification of monoclonal immunoglobulins and quantitative immunoglobulin abnormalities in hairy cell leukemia and chronic lymphocytic leukemia. Am. J. Clin. Pathol. 102:580-585.
16. Beaume, A., A. Bizard, B. Dreyfus, and J.-L. Preud'homme. 1994. High incidence of serum monoclonal Igs detected by a sensitive immunoblotting technique in B-cell chronic lymphocytic leukemia. Blood. 84:1216-1219.
17. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
18. Kabat, E.A., T.T. Wu, H.M. Perry, K.S. Gottesman, and C. Foeller. 1991. Sequences of proteins of immunological interest (ed. 5). US Department of Health and Human Services, Bethesda, MD.
19. Efremov, D.G., F.D. Batista, and O.R. Burrone. 1993. Molecular analysis of IgE H-chain transcripts expressed in vivo by peripheral blood lymphocytes from normal and atopic individuals. J. Immunol. 151:2195-2207.
20. H region repertoire in chronic lymphocytic leukemia patients with autoimmune hemolytic anemia. Blood. 87:3869-3876.
21. H segments with different hypervariable loops. J. Mol Biol. 227:776-798.
22. Deane, M., and J.D. Norton. 1990. Detection of immunoglobulin gene rearrangement in B lymphoid malignancies by polymerase chain reaction gene amplification. Br. J. Haematol. 74:251-256.
23. Franzin, F., D.G. Efremov, G. Pozzato, P. Tulissi, F. Batista, and O.R. Burrone. 1995. Clonal B-cell expansions in peripheral blood of HCV-infected patients. Br. J. Haematol. 90:548-552.
24. Baumann, B., M.J. Potash, and G. Kohler. 1985. Consequences of frameshift mutations at the immunoglobulin heavy chain locus of the mouse. EMBO J. 4:351-359.
25. Lozano, F., B. Maertzdorf, R. Pannell, and C. Milstein. 1994. Low cytoplasmatic mRNA levels of immunoglobulin κ light chain genes containing nonsense codons correlate with inefficient splicing. EMBO J. 13:4617-4622.
26. Bhan, A.K., L.M. Nadler, P. Stashenko, R.T. McCluskey, and S.F. Schlossman. 1981. Stage of B cell differentiation in human lymphoid tissue. J. Exp. Med. 154:737-749.
27. Jackson, D.G., and J.I. Bell. 1990. Isolation of a cDNA encoding the human CD38(T10) molecule cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation. J. Immunol. 144: 2811-2815.
28. Newman, R.A., B. Peterson, F.R. Davey, C. Brabyn, H. Collins, V.L. Brunetto, D.B. Duggan, R.B. Weiss, I. Royston, F.E. Millard, A.A. Miller, and C.D. Bloomfield. 1993. Phenotypic markers and BCL-1 gene rearrangements in B-cell chronic lymphocytic leukemia: a cancer and leukemia group B study. Blood. 82:1239-1246.
29. Jacob, J., G. Kelsoe, K. Rajewsky, and U. Weiss. 1991. Intraclonal generation of antibody mutants in germinal centres. Nature (Lond.). 354:389-392.
30. L regions expressed in IgG-bearing chronic lymphocytic leukemia (CLL): Further evidence that CLL is a heterogenous group of tumors. Blood. 82:1626-1631.
31. Wakai, M., S. Hashimoto, M. Omata, Z.M. Sthoeger, S.L. Allen, S.M. Lichtman, P. Schulman, V.P. Vinciguerra, B. Diamond, M. Dono, M. Ferrarini, and N. Chiorazzi. 1994. IgG+, CD5+ human chronic lymphocytic leukemia B cells. Production of IgG antibodies that exhibit diminished autoreactivity and IgG subclass skewing. Autoimmunity. 19:39-48.
32. Sideras, P., L. Nilsson, K.B. Islam, I.Z. Quintana, L. Friehof, A. Rosen, G. Juliusson, L. Hammarstrom, and C.I.E. Smith. 1992. Transcription of unrearranged Ig H chain genes in human B cell malignancies: Biased expression of genes encoded within the first duplication unit of the Ig H chain locus. J. Immunol. 149:244-252.
33. DePinho, R., K. Kruger, N. Andrews, S. Lutzker, D. Baltimore, and F.W. Alt. 1984. Molecular basis of heavy-chain class switching and switch region deletion in an Abelson virus-transformed cell line. Mol. Cell. Biol. 4:2905-2910.
34. Radbruch, A., W. Muller, and K. Rajewsky. 1986. Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts. Proc. Natl. Acad. Sci. USA. 83:3954-3957.
35. Engelfriet, C.P., M.A.M. Overbeeke, and A.E.G.K. von dem Borne. 1992. Autoimmune hemolytic anemia. Sem. Hematol. 29:3-12.
36. Faguet, G.B., and J.F. Agee. 1992. CD5 and cCLLa expression in chronic lymphocytic leukemia (CLL): Demonstration of their relative prevalence and that of other common B-CLL markers. Leuk. Lymphoma. 6:335-344.
37. Hardy, R.R., and K. Hayakawa. 1993. CD5 B cells, a fetal B cell lineage. Adv. Immunol. 55:297-339.
38. Kipps, T.J., and D.A. Carson. 1993. Autoantibodies in chronic lymphocytic leukemia and related systemic autoimmune diseases. Blood. 81:2475-2487.
39. H5 gene in human B cell chronic lymphocytic leukemia. J. Exp. Med. 176:1073-1081.
40. Schroeder, H.W. Jr., and G. Dighiero. 1994. The pathogenesis of chronic lymphocytic leukemia: analysis of the antibody repertoire. Immunol. Today. 15:288-294.
41. H genes expressed by CD5-negative chronic lymphocytic leukemia producing pathogenic IgM rheumatoid factor. J. Immunol. 144:1526-1529.
42. - chronic lymphocytic leukemia B cells. J. Exp. Med. 181:1507-1517.