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125I]iodo-2′-deoxyundine. Isotope incorporated by the spleens of the recipients was determined the next day after extensive rinsing of the spleens with phosphate-buffered saline.
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We thank E. Long and D Raulet for helpful comments on the manuscript, D Littman for the CD4 mutant mice; M. Digelmann, J -M. Kuhry, P. André, and M. Duval for help in creating the knockout mice, C. Schricke and F. Fischer for help with care of the mice; P. Gerber and C. Ebel for assistance; P. Eberling and F. Ruffenach for peptides and oligonucleotides; and C. Waltzinger for help with flow cytometry. Supported by funds from INSERM, CNRS, and the Centre Hospitalier Universitaire Régional, and by grants to D.M. and C B from the Association pour la Recherche sur le Cancer and the Human Frontier Science Program. T.M. was supported by a fellowship from the Human Frontier Science Program Organization.
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