Increased polymerase fidelity of E89G, a nucleoside analog-resistant variant of human immunodeficiency virus type 1 reverse transcriptase

Journal of Virology

Volumn 70, Issue 7, 1996, Pages 4834-4838

  Drosopoulos, William C ^a   Prasad, Vinayaka R ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEOTIDE; PRIMER DNA; RNA DIRECTED DNA POLYMERASE;

ARTICLE; BASE PAIRING; DNA TEMPLATE; DRUG RESISTANCE; GENETIC VARIABILITY; HUMAN IMMUNODEFICIENCY VIRUS 1; PRIORITY JOURNAL; VIRUS MUTATION;

EID: 0030000114     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.70.7.4834-4838.1996     Document Type: Article

References (41)

1. Beard, W. A., S. J. Stahl, H. R. Kim, K. Bebenek, A. Kumar, M. P. Strub, S. P. Becerra, T. A. Kunkel, and S. A. Wilson. 1994. Structure/function studies of human immunodeficiency virus type 1 reverse transcriptase. Alanine scanning mutagenesis of an alpha-helix in the thumb subdomain. J. Biol. Chem. 269:28091-28097.
2. Bebenek, K., J. Abbots, J. D. Roberts, S. H. Wilson, and T. A. Kunkel. 1989. Specificity and mechanism of error-prone replication by human immunodeficiency virus-1 reverse transcriptase. J. Biol. Chem. 264:16948-16956.
3. Bebenek, K., W. A. Beard, J. R. Casas-Finet, H. R. Kim, T. A. Darden, S. H. Wilson, and T. A. Kunkel. 1995. Reduced frameshift fidelity and processivity of HIV-1 reverse transcriptase mutants containing alanine substitutions in helix H of the thumb subdomain. J. Biol. Chem. 270:19516-19523.
4. Bebenek, K., and T. A. Kunkel. 1993. The fidelity of retroviral reverse transcriptase. p. 85-102. In A. M. Skalka and S. P. Goff (ed.), Reverse transcriptase. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
5. Boosalis, M. S., J. Petruska, and M. F. Goodman. 1987. DNA polymerase insertion fidelity. J. Biol. Chem. 262:14689-14696.
6. Boyer, P. L., C. Tantillo, A. Jacobo-Molina, R. G. Nanni, J. Ding, E. Arnold, and S. H. Hughes. 1994. Sensitivity of wild-type human immunodeficiency virus type 1 reverse transcriptase to dideoxynucleotides depends on template length: the sensitivity of drug-resistant mutants does not. Proc. Natl. Acad. Sci. USA 91:4882-4886.
7. Cheng, Y.-C., G. E. Dutschman, K. F. Bastow, M. G. Sarngadharan, and R. Y. C. Ting. 1987. Human immunodeficiency virus reverse transcriptase. J. Biol. Chem. 262:2187-2189.
8. Cleland, W. W. 1979. Statistical analysis of enzyme kinetic data. Methods Enzymol. 63:103-138.
9. Coffin, J. M. 1995. HIV population dynamics in vivo: implications for genetic variation, pathogenesis and therapy. Science 267:483-489.
10. Creighton, S., L. B. Bloom, and M. F. Goodman. 1995. Gel fidelity assay measuring nucleotide misinsertion, exonucleolytic proofreading, and lesion bypass efficiencies. Methods Enzymol. 262:232-256.
11. Gao, Q., Z. Gu, M. A. Parniak, J. Cameron, N. Cammack, C. Boucher, and M. A. Wainberg. 1993. The same mutation that encodes low-level human immunodeficiency virus type I resistance to 2′,3′-dideoxyinosine and 2′,3′-dideoxycytidine confers high-level resistance to the (-) enantiomer of 2′,3′-dideoxy-3′-thiacytidine. Antimicrob. Agents Chemother. 37:1390-1392.
12. Hubner, A., M. Kruhnoffer, F. Grosse, and G. Krauss. 1992. Fidelity of human immunodeficiency virus type 1 reverse transcriptase in copying natural RNA. J. Mol. Biol. 223:595-600.
13. Jacobo-Molina, A., J. Ding, R. G. Nanni, A. D. J. Clark, X. Lu, C. Tantillo, R. L. Williams, G. Kamer, A. L. Ferris, P. Clark, A. Hizi, S. Hughes, and E. Arnold. 1993. Crystal structure of human immunodeficiency virus reverse transcriptase complexed with double stranded DNA at 3.0 Å resolution shows bent DNA. Proc. Natl. Acad. Sci. USA 90:6320-6324.
14. Kedar, P. S., J. Abbotts, T. Kovacs, K. Lesiak, P. Torrence, and S. H. Wilson. 1990. Mechanism of HIV reverse transcriptase: enzyme-primer interaction as revealed through studies of a dNTP analogue, 3′-azido-dTTP. Biochemistry 29:3603-3611.
15. Kew, Y., Q. Song, and V. Prasad. 1994. Subunit selective mutagenesis of Glu89 residue in human immunodeficiency virus reverse transcriptase. J. Biol. Chem. 269:15331-15336.
16. Kohlstaedt, L. A., J. Wang, J. M. Friedman, P. A. Rice, and T. A. Steitz. 1992. Crystal structure at 3.5 Å resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science 256:1783-1790.
17. Lacey, S. F., J. E. Reardon, E. S. Furfine, T. A. Kunkel, K. Bebenek, K. A. Eckert, S. D. Kemp, and B. A. Larder. 1992. Biochemical studies on the reverse transcriptase and RNase H activities from human immunodeficiency virus strains resistant to 3′-azido-3′-deoxythymidine. J. Biol. Chem. 267: 15789-15794.
18. Larder, B. A. 1993. Inhibitors of HIV reverse transcriptase as antiviral agents and drug resistance, p. 205-222. In A. M. Skalka and S. P. Goff (ed.), Reverse transcriptase. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
19. Larder, B. A., G. Darby, and D. D. Richman. 1989. HIV with reduced sensitivity to zidovudine (AZT) isolated during prolonged therapy. Science 243:1731-1734.
20. Larder, B. A., and S. D. Kemp. 1989. Multiple mutations in HIV-1 reverse transcriptase confer high-level resistance to zidovudine (AZT). Science 246: 1155-1158.
21. Le Grice, S. F. J., and F. Gruninger-Leitch. 1990. Rapid purification of homodimer and heterodimer HIV-1 reverse transcriptase by metal chelate affinity chromatography. Eur. J. Biochem. 187:307-314.
22. Mendelman, L. V., M. S. Boosalis, J. Petruska, and M. F. Goodman. 1989. Nearest neighbor influences on DNA polymerase insertion fidelity. J. Biol. Chem. 264:14415-14423.
23. Mendelman, L. V., J. Petruska, and M. F. Goodman. 1990. Base mispair extension kinetics. J. Biol. Chem. 265:2338-2346.
24. Pandey, V. N., N. Kaushik, N. Rege, S. G. Sarafianos, P. N. S. Yadav, and M. J. Modak. 1996. Role of methionine 184 of human immunodeficiency virus type 1 reverse transcriptase in the polymerase function and fidelity of DNA synthesis. Biochemistry 35:2168-2179.
25. Perrino, F. W., B. D. Preston, L. L. Sandell, and L. A. Loeb. 1989. Extension of mismatched 3′ termini of DNA is a major determinant of the infidelity of human immunodeficiency virus type 1 reverse transcriptase. Proc. Natl. Acad. Sci. USA 86:8343-8347.
26. Petruska, J., M. F. Goodman, M. S. Boosalis, L. C. Sowers, C. Cheong, and J. I. Tinoco. 1988. Comparison between DNA melting thermodynamics and DNA polymerase fidelity. Proc. Natl. Acad. Sci. USA 85:6252-6256.
27. Prasad, V. R., and S. P. Goff. 1989. A novel in situ colony screening method to detect human immunodeficiency virus reverse transcriptase activity expressed in bacteria. J. Biol. Chem. 264:16689-16693.
28. Prasad, V. R., I. Lowy, T. De Los Santos, L. Chiang, and S. P. Goff. 1991. Isolation and characterization of a dideoxyguanosine triphosphate-resistant HIV-1 reverse transcriptase expressed in bacteria. Proc. Natl. Acad. Sci. USA 88:11363-11367.
29. Preston, B. D., and N. Garvey. 1992. Retroviral mutation and reverse transcriptase fidelity. Pharm. Technol. 16:34-52.
30. Preston, B. D., B. J. Poiesz, and L. A. Loeb. 1988. Fidelity of HIV-1 reverse transcriptase. Science 242:1168-1171.
31. Reardon, J. E., and W. Miller. 1990. Human immunodeficiency virus reverse transcriptase: substrate and inhibitor kinetics with thymidine 5′ triphosphate and 3′-azido-3′ deoxythymidine 5′-triphosphate. J. Biol. Chem. 265:20302-20307.
32. Ricchetti, M., and H. Buc. 1990. Reverse transcriptases and genomic variability: the accuracy of DNA replication is enzyme specific and sequence dependent. EMBO. J. 9:1583-1593.
33. Richman, D. D., J. M. Grimes, and S. W. Lagakos. 1990. Effect of stage of disease and drug dose on zidovudine susecptibilities of isolates of human immunodeficiency virus. Acquired Immune Defic. Syndr. 3:743-746.
34. Roberts, J. D., K. Bebenek, and T. A. Kunkel. 1988. The accuracy of reverse transcriptase from HIV-I. Science 242:1171-1173.
35. Sarafianos, S. G., V. N. Pandey, N. Kaushik, and M. J. Modak. 1995. Glutamine 151 participates in the substrate dNTP binding function of HIV-1 reverse transcriptase. Biochemistry 34:7207-7216.
36. Shirasaka, T., R. Yarchoan, M. C. O'Brien, R. N. Husson, B. D. Anderson, E. Kojima, T. Shimada, S. Border, and H. Mitsuya. 1993. Changes in drug sensitivity of human immunodeficiency virus type 1 during therapy with azidothymidine, dideoxycytidine, and dideoxyinosine: an in vitro comparative study. Proc. Natl. Acad. Sci. USA 90:562-566.
37. Song, Q., G. Yang, S. P. Goff, and V. R. Prasad. 1992. Mutagenesis of the Glu-89 residue in human immunodeficiency virus type 1 (HIV-1) and HIV-2 reverse transcriptases: effects on nucleoside analog resistance. J. Virol. 66: 7568-7571.
38. Tantillo, C., J. Ding, A. Jacobo-Molina, R. G. Nanni, P. L. Boyer, S. H. Hughes, R. Pauwels, K. Andries, P. A. J. Janssen, and E. Arnold. 1994. Locations of anti-AIDS drug binding sites and resistance mutations in the three-dimensional structure of HIV-1 reverse transcriptase. J. Mol. Biol. 243:369-387.
39. Wainberg, M. A., W. C. Drosopoulos, H. Salomon, M. Hsu, G. Borkow, M. A. Parniak, Z. Gu, Q. Song, J. Manne, S. Islam, G. Castriota, and V. R. Prasad. 1996. Enhanced fidelity of 3TC-selected mutant HIV-1 reverse transcriptase. Science 271:1282-1285.
40. Weber, J., and F. Grosse. 1989. Fidelity of human immunodeficiency virus type 1 reverse transcriptase in copying natural DNA. Nucleic Acids Res. 17:1379-1393.
41. Yu, H., and M. F. Goodman. 1992. Comparison of HIV-1 and avian myeloblastosis virus reverse transcriptase fidelity on RNA and DNA templates. J. Biol. Chem. 267:10888-10896.