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Volumn 274, Issue 5288, 1996, Pages 782-784

An essential role for NF-κB in preventing TNF-α-induced cell death

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR RECEPTOR;

EID: 0029976817     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5288.782     Document Type: Article
Times cited : (2966)

References (33)
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    • (1991) Science , vol.251 , pp. 1490
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    • note
    • 7 U/mg) was used.
  • 17
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    • Wiley, New York
    • -/- embryos were disrupted by passage through a syringe and plated overnight in DMEM with 20% fetal bovine serum and 30% of total volume of L929 fibrosarcoma-conditioned media (containing macrophage growth factors) to allow attachment of adherent cells. Cells present in the supernatant were then plated on a six-well plate. Within 2 days macrophage colonies appeared. Cells obtained in this way are positive for the macrophage-specific marker Mac-1. TNF-α was added when the plates were 50% confluent, for periods indicated in the text. After TNF-α treatment, cells were treated with Dispase II (Boehringer Mannheim) and scraped from the plates. Viable cells were counted by trypan blue exclusion.
    • (1994) Current Protocols in Immunology
    • Coico, R.1
  • 18
    • 0027168948 scopus 로고
    • -/- 3T3 cells were plated as described in (13). Twenty-four hours later they were incubated with a calcium phosphate-DNA precipitate solution for 3 hours, after which the cells were treated with glycerol (Hepes-buffered saline with 15% glycerol) and then fresh media added. Thirty-six hours later TNF-α was added to the cells for 24 hours, after which they were washed with phosphate-buffered saline (PBS), fixed, and then treated with an X-Gal (5-bromo-4-chloro-3-indoxyl-β-D-galactopyranoside) staining solution. Three hours later blue cells were counted in 10 independent views of the plates. The plasmid pON 405, in which LacZ expression is driven by the cytomegalovirus promoter, was used to mark transfected cells. The plasmid pGD-p65 [M. Scott et al., Genes Dev. 7, 1266 (1993)] was used for expression of ReIA in fibroblasts. The parental pGD vector was used to ensure that the total amount of DNA used in all transfections was identical.
    • (1993) Genes Dev. , vol.7 , pp. 1266
    • Scott, M.1
  • 21
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    • M. Muzio et a/., ibid. 85, 817 (1996).
    • (1996) Cell , vol.85 , pp. 817
    • Muzio, M.1
  • 33
    • 10544228651 scopus 로고    scopus 로고
    • note
    • We thank L. Herzenberg (Stanford University) for the plasmid pON 405, A. Gitford for technical assistance, and members of our laboratory for discussions and comments. A.A.B. was supported by a Concern II-Cancer Research Institute Fellowship. D.B. is an American Cancer Society Research Professor. Supported by a grant from the Amgen Corporation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.