Comparative analysis of flow cytometric methods for apoptosis quantitation in murine thymocytes and human peripheral lymphocytes from controls and HIV-infected persons. Evidence for interference by granulocytes and erythrocytes

Journal of Immunological Methods

Volumn 198, Issue 1, 1996, Pages 87-99

  Lecoeur, Hervé ^a   Gougeon, Marie Lise ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Apoptosis; Erythrocyte; Flow cytometry; Granulocyte; Human lymphocyte; Thymocyte

Indexed keywords

ADULT; APOPTOSIS; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; ERYTHROCYTE; FLOW CYTOMETRY; GRANULOCYTE; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; PRIORITY JOURNAL; THYMOCYTE;

HUMAN IMMUNODEFICIENCY VIRUS; MURINAE;

EID: 0029961387     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00148-2     Document Type: Article

References (29)

1. Ameisen, J.C. and Capron, A. (1991) Cell dysfunction and depletion in AIDS: the programmed cell death hypothesis. Immunol Today 12, 102.
2. Arends, M.J. and Wyllie, A.H. (1991) Apoptosis: mechanisms and roles in pathology. Int. Rev. Exp. Pathol. 32, 223.
3. Baffy, G., Miyashita, T., Williamson, J.R. and Reed, J.C. (1993) Apoptosis induced by withdrawal of interleukin-3 (IL-3) from an IL-3 dependent hematopoietic cell line is associated with repartitioning of intracellular calcium and is blocked by enforced bcl-2 oncoprotein production. J. Biol. Chem. 268, 6511.
4. Blackman, M., Kappler, J.A. and Marrack, P. (1990) The role of the T-cell receptor in positive and negative selection of developing T cells. Science 248, 1335.
5. + T lymphocytes during HIV-infection. J. Immunol., in press.
6. Brown, D.G., Sun, X.M. and Cohen, G.M. (1993) Dexamethasone-induced apoptosis involves cleavage of DNA to large fragments prior to internucleosomal fragmentation. J. Biochem. Chem 268, 3037.
7. Darzynkiewicz, Z., Bruno, S., Del Bino, G., Gorczyca, W., Hotz, M.A., Lassota, P. and Traganos, F. (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13, 795.
8. Finkel, T.H., Tudor-Williams, G., Banda, N.K., Cotton, M.F., Curiel, T. et al. (1995) Apoptosis occurs predominantly in bystander cells and not in productively infected cells of HIV-and SIV-infected lymph nodes. Nat. Med. 2, 129.
9. Gougeon (1995) Does apoptosis contribute to CD4 T cell depletion in human immunodeficiency virus infection? Cell Death Diff. 2, 1.
10. Gougeon, M.L., Lecoeur, H., Dulioust, A., Enouf, M.G., Crouvoisier, M., Goujard, C., Debords, T. and Montagnier, L. (1996) Programmed Cell Death in peripheral lymphocytes from HIV-infected persons: the increased susceptibility to apoptosis of CD4 and CDS T cells correlates with lymphocyte activation and with disease progression. J. Immunol. 156, 3509.
11. Gougeon, M.L., Garcia, S., Heeney, J., Tschopp, R., Lecoeur, H., Guetard, D., Rame, V., Dauguet, C. and Montagnier, L. (1993) Programmed cell death in AIDS-related HIV and SIV infections. Aids Res. Hum. Retrovir. 9, 553.
12. Gougeon, M.L., Olivier, R., Garcia, S., Guetard, D., Dragic, T., Dauguet, C. and Montagnier, L. (1991) Demonstration of an engagement process towards cell death by apoptosis in lymphocytes of HIV infected patients. C.R. Acad. Sci. Ser. Iii 312, 529.
13. Jonker, R. (1993) In: A. Jacquemin-Sablon (Ed.), Detection of Apoptosis using Fluorescent In Situ Nick Translation. Springer-Verlag, Berlin, H 67, 355.
14. + T cells from HIV-infected persons. J. Immunol. 153.
15. Loken, M.R., Shah, V.O., Dattilio, K.L. and Civin, C.I. (1987) Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood 69, 255.
16. Meyaard, L., Otto, S.A., Jonker, R.R., Mijnster, M.J., Gougeon, M.L., Keet, R., and Miedema, F. (1992) Programmed death of T cells in HIV-1 infection. Science 257, 217.
17. Meyaard, L., Otto, S.A., Keet, I.P., Roos, M.T. and Miedema, F. (1994) Programmed death of T cells in human immunodeficiency virus infection. No correlation with progression to disease. J. Clin. Invest. 93, 982.
18. Muro-Cacho, C.A., Pantaleo, G. and Fauci, A. (1995) Intensity of apoptosis correlates with the general state of activation of the lymphoid tissue and not with the stage of disease or viral burden. J. Immunol. 154, 5555.
19. Nicoletti, I., Migliorati, G., Pagliacci, M.C., Grignani, F. and Riccardi, C. (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J. Immunol. Methods 139, 271.
20. Olivier, R. (1995). Flow cytometry technique for assessing effects of N-Acetylcysteine on apoptosis and cell viability of human immunodeficiency virus-infected lymphocytes. Methods Enzymol. 251, 270.
21. Petit, P.X., Lecoeur, H., Zorn, E., Dauguet, C., Mignotte, B. and Gougeon, M.L. (1995) Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J. Cell. Biol. 130, 157.
22. Raffay, M. and Cohen, G.M. (1991) Bis-(tri-n-butylin) oxide induces programmed cell death (apoptosis) in immature mouse thymocytes. Arch. Toxicol. 65, 135.
23. Schmid, I., Uittenbogaart, C.H., Keld, B. and Giorgi, J.V. (1994) A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170, 145.
24. Stern, M., Meagher, L., Savill, J. and Haslett, C. (1992) Apoptosis in human eosinophils. Programmed cell death in the eosinophil leads to phagocytosis by macrophages and is modulated by IL-5. J. Immunol. 148, 3543.
25. Sun, X.M., Snowden, R.T., Skilleter, D.N., Dinsdale, D., Ormerod, M.G. and Cohen, G.M. (1992a). A flow-cytometric method for the separation and quantitation of normal and apoptotic thymocytes. Anal. Biochem. 204, 351.
26. Sun, X.M., Dinsdale, D., Snowden, R.T., Cohen, G.M. and Skilleter, D.N. (1992b) Characterization of apoptosis in thymocytes isolated from dexamethasone-treated rats. Biochem. Pharmacol. 44, 2131.
27. + thymocytes by flow cytometry. J. Immunol. Methods 137, 79.
28. Terstappen, L.W., Hollander, Z., Meiners, H. and Loken, M.R. (1990) Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J. Leukoc. Biol. 48, 138.
29. Torres-Roca, J.F., Lecoeur, H., Amatore, C. and Gougeon, M.L. (1995) The early production of a reactive oxygen intermediate mediates apoptosis in dexamethasone-treated thymocytes. Cell Death Diff. 2, 309.