Common IgE-epitopes of recombinant Phl p I, the major timothy grass pollen allergen and natural group I grass pollen isoallergens

Molecular Immunology

Volumn 33, Issue 4-5, 1996, Pages 417-426

  Laffer, Sylvia ^a   Duchene, Michael ^a   Reimitzer, Irmgard ^a   Susani, Markus ^b   Mannhalter, Christine ^a   Kraft, Dietrich ^a   Valenta, Rudolf ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b Institute of Molecular Biology Salzburg   (Austria)

Author keywords

grass pollen allergy; IgE; Phl p I; recombinant allergen

Indexed keywords

EPITOPE; IMMUNOGLOBULIN E;

ANTIGEN DETECTION; ANTIGEN EXPRESSION; ARTICLE; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; GRASS POLLEN; IMMEDIATE TYPE HYPERSENSITIVITY; POLLEN ALLERGY; PRIORITY JOURNAL; TWO DIMENSIONAL ELECTROPHORESIS;

ESCHERICHIA COLI; PHLEUM PRATENSE;

EID: 0029999353     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/0161-5890(95)00152-2     Document Type: Article

References (32)

1. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248-254.
2. Breiteneder H., Ferreira F., Hoffmann-Sommergruber K. and Scheiner O. (1993) Four recombinant isoforms of Cor a I, the major allergen of hazel pollen, show different IgE-binding properties. Eur. J. Biochem. 212, 355-362.
3. Breiteneder H., Pettenburger K., Bito A. and Breitenbach M. (1989) The gene coding for the major birch pollen allergen, Bet v I, is highly homologous to a pea disease resistance response gene. EMBO J. 8, 1935-1938.
4. Dolecek C., Vrtala S., Laffer S., Steinberger P., Kraft D., Scheiner O. and Valenta R. (1993) Molecular characterization of Phl p II, a major timothy grass (Phleum pratense) pollen allergen. FEBS Lett. 335, 299-304.
5. Esch R. E. and Klapper D. G. (1989) Isolation and characterization of a major cross-reactive grass group I allergenic determinant. Molec. Immun. 26, 557-561.
6. Fling S. P. and Gregerson D. S. (1986) Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris buffer system without urea. Analyt. Biochem. 155, 83-88.
7. Freidhoff L. R., Ehrlich-Kautzky E., Grant J. H., Meyers D. A. and Marsh D. G. (1986) A study of the human immune response to Lolium perenne (rye) pollen and its components, Lol p I and Lol p II (rye I and rye II). J. Allergy clin. Immun. 78, 1190-1201.
8. Griffith I. J., Smith P. M., Pollock J., Theerakulpisut P., Avjioglu A., Davies S., Hough T., Singh M. B., Simpson R. J., Ward L. D. and Knox R. B. (1991) Cloning and sequencing of Lol p I, the major allergenic protein of rye grass pollen. FEBS Lett. 279, 210-215.
9. Hirschwehr R., Valenta R., Ebner C., Ferreira F., Sperr W. R., Valent P., Rohac M., Rumpold H., Scheiner O. and Kraft D. (1992) Identification of common allergenic structures in hazel pollen and hazelnuts: a possible explanation for sensitivity to hazelnuts in patients allergic to tree pollen. J. Allergy clin. Immun. 90, 927-936.
10. Laffer S., Vrtala S., Duchêne M., vanRee R., Kraft D., Scheiner O. and Valenta R. (1994) IgE-binding capacity of recombinant timothy grass (Phleum pratense) pollen allergens. J. Allergy clin. Immun. 94, 88-94.
11. Laffer S., Valenta R., Vrtala S., Susani M., vanRee R., Kraft D., Scheiner O. and Duchêne M. (1994) Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE-binding to group I allergens from eight different grass species. J. Allergy clin. Immun. 94, 689-698.
12. Larsen J. N., Stroman P. and Ipsen H. (1992) PCR based cloning and sequencing of isogenes encoding the tree pollen major allergen Car b I from Carpinus betulus, hornbeam. Molec. Immun. 29, 703-711.
13. + gene, expressed under the control of the mating-type locus, induces meiosis and sporulation in fission yeast. EMBO J. 6, 729-736.
14. Perez M., Ishioka G. Y., Walker L. E. and Chesnut R. W. (1990) cDNA cloning and immunological characterization of the rye grass allergen Lol p I. J. biol. Chem. 265, 16,210-16,215.
15. Rafnar T., Griffith I. J., Kuo M. C., Bond J. F., Rogers B. L. and Klapper D. G. (1991) Cloning of Amb a I (antigen E), the major allergen family of short ragweed pollen. J. biol. Chem. 266, 1229-1236.
16. Rohac M., Birkner T., Reimitzer I., Bohle B., Steiner R., Breitenbach M., Kraft D., Scheiner O., Gabi F. and Rumpold H. (1991) The immunological relationship of epitopes on major tree pollen allergens. Molec. Immun. 28, 897-906.
17. Sammons D. W., Adams L. D. and Nishizawa E. E. (1981) Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2, 135-141.
18. Schenk S., Hoffmann-Sommergruber K., Breiteneder H., Ferreira F., Fischer G., Scheiner O., Kraft D. and Ebner C. (1994) Four recombinant isoforms of Cor a 1, the major allergen of hazel pollen, show different reactivities with allergen-specific T-lymphocyte clones. Eur. J. Biochem. 224, 717-722.
19. Scheiner O. and Kraft D. Basic and practical aspects of recombinant allergens, Allergy, in press.
20. Schenk S., Breiteneder H., Susani M., Najafian N., Laffer S., Duchêne M., Valenta R., Fischer G., Scheiner O., Kraft D. and Ebner C. T cell epitopes of Phl p 1, the major allergen of timothy grass (Phleum pratense); crossreactivity with group I allergens from other grass species. J. Allergy clin. Immun. in press.
21. Silvanovich A., Astwood J., Zhang L., Olsen E., Kisil F., Sehon A., Mohapatra S. and Hill R. (1991) Nucleotide sequence analysis of three cDNAs coding for Poa p IX isoallergens of Kentucky bluegrass pollen. J. biol. Chem. 266, 1204-1210.
22. Staiger C. J., Goodbody K. C., Hussey P. J., Valenta R., Drobak B. K. and Lloyd C. W. (1993) The profilin multigene family of maize: differential expression of three isoforms. Plant J. 4, 631-641.
23. Studier F. W., Rosenberg A. H., Dunn J. J. and Dubendorff J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Meth. Enzymol. 185, 60-89.
24. Swoboda I., Jilek A., Ferreira F., Engel E., Hoffmann-Sommergruber K., Scheiner O., Kraft D., Breiteneder H., Pittenauer E., Schmid E., Vicente O., Heberle-Bors E., Ahorn H. and Breitenbach M. (1995) Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry, and cDNA cloning. J. biol. Chem. 270, 2607-2613.
25. Towbin H., Staehelin T. and Gordon J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. natn. Acad. Sci. U.S.A. 76, 4350-4354.
26. Valenta R., Vrtala S., Ebner C., Kraft D. and Scheiner O. (1992) Diagnosis of grass pollen allergy with recombinant timothy grass (Phleum pratense) pollen allergens. Int. Arch. Allergy Immun. 97, 287-294.
27. Valenta R., Ferreira F. and Grote M. (1993) Identification of profilin as an actin-binding protein in higher plants. J. biol. Chem. 268, 22777-22778
28. Valenta R., Dolecek C., Menz G., Schönheit-Kenn U., Susani M., Grönlund H. and Kraft D. (1994) Diagnostic and therapeutic concepts based on recombinant allergens. In Progress in Allergy and Clinical Immunology, pp. 226-233. Hogrefe and Huber Publishers.
29. Valenta R., Vrtala S., Ball T., Laffer S., Steinberger P. and Kraft D. (1995) Allergen-specific therapy in Type I allergy: New concepts based on recombinant allergens. ACI News 6, 165-169.
30. Vrtala S., Sperr W. R., Reimitzer I., vanRee R., Laffer S., Müller W. D., Valent P., Lechner K., Rumpold H., Kraft D., Scheiner O. and Valenta R. (1993) cDNA cloning of a major allergen from timothy grass (Phleum pratense) pollen, PUp V; characterization of the recombinant Phl p V allergen. J. Immun. 151, 4773-4781.
31. Vrtala S., Susani M., Sperr W. R., Valent P., Laffer S., Dolecek C., Kraft D. and Valenta R. Immunological characterization of purified recombinant timothy grass pollen (Phleum pratense) allergens (Phl p 1, Phl p 2, Phl p 5), J. Allergy clin. Immun. in press.
32. Wüthrich B. (1989) Epidemiology of the allergic diseases. Are they really on the increase? Int. Arch. Allergy appl. Immun. 90, 3-10.