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Volumn 274, Issue 5290, 1996, Pages 1199-1201

Identification of BIME as a subunit of the anaphase-promoting complex

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN B; PROTEIN SUBUNIT; UBIQUITIN PROTEIN LIGASE;

EID: 0029909251     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.274.5290.1199     Document Type: Article
Times cited : (175)

References (39)
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    • 2, 475 mM KCl, and 0.5% NP40. Ubiquitination assays with APC bound to antibody beads and elution of APC from the beads for SDS-polyacrylamide gel electrophoresis (PAGE) analysis were done as described (5). APC preparations from two different mitotic extracts (Δ90 extracts and extracts from metaphase II-arrested eggs) (5) had identical subunit compositions and ubiquitination activities.
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    • 2, and 100 μg/ml bovine serum albumin (BSA)] containing either 33 μg γ-PPase (300 units per microgram; Biolabs) or no γ-PPase. BSA and phosphatase were removed by washing the beads as described (9).
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    • note
    • We thank P. Hieter, S. Tugendreich, A. Page, and T. Rapoport for antibodies: K. Nasmyth, W. Zachariae, and T. Shin for communicating unpublished results: H. Yu for recombinant UBCx; A. Georgi and T. Bernai for assistance with immunoblots; E. Brundell for help with the Tsg24 antibodies; and T. Enoch. M. O'Connell, and A. Amon for discussions. Supported by fellowships from the European Molecular Biology Organization (J.-M.P.) and by grants from the Swedish Cancer Society (C.H.), the Kjell and Marta Beijers Stiftelse (C.H.), and the National Institute of General Medical Sciences (M.W.K.).


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