Isolation of glycosylation mutants in Dictyostelium discoideum using flow cytometry

Cytometry

Volumn 25, Issue 2, 1996, Pages 133-143

  Griffiths, Katherine R ^a   Champion, Alan C ^a   Vesey, Graham ^a   Williams, Keith L ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

Dictyostelium; Flow cytometry; O glycosylation mutants; Restriction enzyme mediated integration; Spore differentiation

Indexed keywords

AMOEBA; ARTICLE; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; FLOW CYTOMETRY; GLYCOSYLATION; MUTATION; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES, MONOCLONAL; DICTYOSTELIUM; EPITOPES; FLOW CYTOMETRY; FUNGAL PROTEINS; GLYCOSYLATION; MEMBRANE PROTEINS; MUTAGENESIS; PROTOZOAN PROTEINS; SPORES;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM;

EID: 0029861378     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19961001)25:2<133::AID-CYTO2>3.0.CO;2-I     Document Type: Article

References (48)

1. Adachi H, Hasebe T, Yoshinaga K, Ohta T, Sutoh K: Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of the blasticidin S resistance marker. Biochem Biophys Res Comm 205:1808-1814, 1994.
2. Alexander S, Smith E, Davis L, Gooley A, Por SB, Browne L, Williams KL: Characterization of an antigenically related family of cell-type specific proteins implicated in slug migration in Dictyostelium discoideum. Differentiation 38:82-89, 1988.
3. Aparicio JG, Erdos GW, West CM: Spore coat is altered in modB glycosylation mutants of Dictyostelium discoideum. J Cell Biochem 42:255-266, 1990.
4. Ballou CE: Isolation, characterization and properties of Saccharomyces cerevisiae mnn mutants with nonconditional protein glycosylation defects. Methods Enzymol 185:440-470, 1990.
5. Bertholdt G, Stadler J, Bozzaro S, Fichtner B, Gerisch G: Carbohydrate and other epitopes of the contact site A glycoprotein of Dictyostelium discoideum as characterized by monoclonal antibodies. Cell Diff 16:187-202, 1985.
6. Browne LH, Sadeghi H, Blumberg D, Williams KL, Klein C: Reexpression of 117 antigen, a cell surface glycoprotein of aggregating cells, during terminal differentiation of Dictyostelium discoideum prespore cells. Development 105:657-664, 1989.
7. Champion A, Gooley AA, Callaghan M, Carrin MI, Bernstein RL, Smith E, Williams KL: Immunodominant carbohydrate determinants in the multicellular stages of Dictyostelium discoideum. J Gen Microbiol 137:2431-2438, 1991.
8. Champion A, Griffiths K, Gooley AA, Gonzalez BY, Gritzali M, West CM, Williams KL: Immunochemical, genetic and morphological comparison of fucosylation mutants of Dictyostelium discoideum. Microbiology 141:785-797, 1995.
9. Cooley L, Kelley R, Spradling A: Insertional mutagenesis of the Drosophila genome with single P elements. Science 239:1121-1128, 1988.
10. Devine KM, Bergmann JE, Loomis WF: Spore coat proteins of Dictyostelium discoideum are packaged in prespore vesicles. Dev Biol 99:437-446, 1983.
11. Dynes J, Firtel R: Molecular Complementation of a genetic marker in Dictyostelium discoideum using a genomic DNA library. Proc Natl Acad Sci USA 86:7966-7970, 1989.
12. Dynes JL, Clark AM, Shaulsky G, Kuspa A, Loomis WF, Firtel RA: LagC is required for cell-cell interactions that are essential for cell-type differentiation in Dictyostelium. Genes Dev 8:948-958, 1994.
13. Erdos GW, West CM: Formation and organization of the spore coat of Dictyostelium discoideum. Exp Mycol 13:169-182, 1989.
14. Firtel RA: Mobile genetic elements in the cellular slime mold Dictyostelium discoideum. In: Mobile DNA, Berg DE, Howe MM (eds). American Society for Microbiology, Washington, DC, 1989, pp 557-566.
15. Fosnaugh K, Fuller D, Loomis WF: Structural roles of the spore coat proteins in Dictyostelium discoideum. Dev Biol 166:823-825, 1994.
16. Free SJ, Schimke RT, Freeze H, Loomis WF: Characterization and genetic mapping of modA, a mutation in the posttranslational modification of the glycosidases of Dictyostelium discoideum. J Biol Chem 253:4102-4106, 1978.
17. Freeze HH: Glycoproteins in Dictyostelium. Dev Genet 11:453, 1990.
18. Freeze HH: Developmental glycobiology of Dictyostelium discoideum. In: Cell Surface Carbohydrates and Cell Development, Fukuda M (ed). CRC Press Inc., Boca Raton, FL, 1991, PP 285-317.
19. Gonzalez-Yanes B, Mandel RB, Girard M, Henry S, Aparicio O, Gritzali M, Brown RD, Jr, Erdos GW, West CM: The spore coat of a fucosylation mutant in Dictyostelium discoideum. Dev Biol 133: 576-587, 1989.
20. Gooley AA, Marshchalek R, Williams KL: Size polymorphisms due to changes in the number of O-glycosylated tandem repeats in the Dictyostelium discoideum glycoprotein PsA. Genetics 130:749-756, 1992.
21. Grant WN, Williams KL: Monoclonal antibody characterisation of slime sheath: the extracellular matrix of Dictyostelium discoideum. Eur Mol Biol Organ J 2:935-940, 1983.
22. Gregg JH, Krefft M, Haas Kraus A, Williams KL: Antigenic differences detected between prespore cells of Dictyostelium discoideum and Dictyostelium mucoroides using monoclonal antibodies. Exp Cell Res 142:229-233, 1982.
23. Hobbie L, Fisher AS, Lee S, Flint A, Krieger M: Isolation of three classes of conditional lethal Chinese hamster ovary cell mutants with temperature-dependent defects in low density lipoprotein receptor stability and intracellular membrane transport. J Biol Chem 269: 20958-20970, 1994.
24. Hubbard SC, Walls L, Ruley HE, Muchmore EA: Generation of chinese hamster ovary cell glycosylation mutants by retroviral insertional mutagenesis. J Biol Chem 269:3717-3724, 1994.
25. Knecht DA, Kessin RH: Recent advances in the molecular genetics of Dictyostelium. Dev Genet 11:388-390, 1990.
26. Krefft M, Voet L, Gregg JH, Mairhofer H, Williams KL: Evidence that positional information is used to establish the prestalk-prespore pattern in Dictyostelium discoideum aggregates. Eur Mol Biol Organ J 3:201-206, 1984.
27. Krefft M, Voet L, Mairhofer H, Williams KL: Analysis of proportion regulation in slugs of Dictyostelium discoideum using a monoclonal antibody and a FACS-IV. Exp Cell Res 147:235-239, 1983.
28. Kuspa A, Loomis WF: Tagging developmental genes in Dictyostelium by restriction enzyme mediated integration of plasmid DNA. Proc Natl Acad Sci USA 89:8803-8807, 1992.
29. Kuspa A, Loomis WF: REMI-RFLP mapping in the Dictyostelium genome. Genetics 138:665-674, 1994.
30. Loomis WF (ed): The development of Dictyostelium discoideum. Academic Press, New York, 1982.
31. Loomis WF: Genetic tools for Dictyostelium discoideum. Methods Cell Biol 28:31-65, 1987.
32. Moerman DG, Waterston RH: Mobile elements in Caenorhabditis elegans and other nematodes. In: Mobile DNA, Berg DE, Howe MM (eds). American Society for Microbiology, Washington, DC, 1989, pp 537-556.
33. Murray BA, Niman HL, Loomis WF: Monoclonal antibody recognizing gp80, a membrane glycoprotein implicated in intercellular adhesion of Dictyostelium discoideum. Mol Cell Biol 3:863-870, 1983.
34. Murray BA, Wheeler S, Jongens T, Loomis WF: Mutations affecting a surface glycoprotein, gp80, of Dictyostelium discoideum. Mol Cell Biol 4:514-519, 1984.
35. Ram AFJ, Wolters A, Hoopen RT, Klis FM: A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast 10:1019-1030, 1994.
36. Smith E, Roberts K, Butcher GW, Galfre G: Monoclonal antibody screening: two methods using antigens immobilized on nitrocellulose. Anal Biochem 138:119-124, 1984.
37. Stanley P: Glycosylation engineering. Glycobiology 2:99-107, 1992.
38. Stanley P, Ioffe E: Glycosyltransferase mutants: key to new insights in glycobiology. Fed Am Soc Exp Biol J 9:1436-1444, 1995.
39. Sussman M: Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions. Methods Cell Biol 28:9-29, 1987.
40. Vesey G, Narai J, Ashbolt N, Williams KL, Veal D: Detection of specific microorganisms in environmental samples using flow cytometry. In: Methods in Cell Biology - Flow Cytometry, Vol 42, Darzynkiewicz Z, Robinson JP, Crissman HA (eds). Academic Press Inc., New York, 1994, pp 490-519.
41. Voet L, Krefft M, Brüderlein M, Williams KL: Flow cytometer study of anterior-like cells in Dictyostelium discoideum. J Cell Sci 75: 423-435, 1985.
42. Watson N, Williams KL, Alexander S: A developmentally regulated glycoprotein complex from Dictyostelium discoideum. J Biol Chem 268:22634-22641, 1993.
43. Watson N, McGuire V, Alexander S: The PsB glycoprotein complex is secreted as a preassembled precursor of the spore coat in Dictyostelium discoideum. J Cell Sci 107:2567-2579, 1994.
44. West CM, Erdos GW: Formation of the Dictyostelium spore coat. Dev Genet 11:492-506, 1990.
45. West CM, Erdos GW: Incorporation of protein into spore coats is not cell autonomous in Dictyostelium. J Cell Biol 116:1291-1300, 1992.
46. West CM, Erdos GW, Davis R: Glycoantigen expression is regulated both temporally and spatially during development in the cellular slime molds Dictyostelium discoideum and D. mucoroides. Mol Cell Biochem 72:121-140, 1986.
47. Williams KL, Emslie KR, Slade MB: Recombinant glycoprotein production in the slime mould DictyosteUum discoideum. Curr Opin Biotechnol 6:538-542, 1995.
48. Zachara NE, Packer NH, Temple MD, Slade MB, Jardine DR, Karuso P, Moss CJ, Mabbutt BC, Curmi PMG, Williams KL, Gooley AA: Recombinant prespore-specific antigen from Dictyostelium discoideum is a β-sheet glycoprotein with a spacer peptide modified by O-linked N-acetylglucosamine. Eur J Biochem 238:511-518, 1996.