Recombinant prespore-specific antigen from Dictyostelium discoideum is a β-sheet glycoprotein with a spacer peptide modified by O-linked N-acetylglucosamine

European Journal of Biochemistry

Volumn 238, Issue 2, 1996, Pages 511-518

  Zachara, Natasha E ^a   Packer, Nicolle H ^a   Temple, Mark D ^b   Slade, Martin B ^a   Jardine, Daniel R ^a   Karuso, Peter ^a   Moss, Catherine J ^a   Mabbutt, Bridget C ^b   Curmi, Paul M G ^b   Williams, Keith L ^a   Gooley, Andrew A ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

Cell surface glycoproteins; Dictyostelium discoideum; N acetylglucosamine; O glycosylation

Indexed keywords

DICTYOSTELIUM DISCOIDEUM; MAMMALIA;

ANTIGEN; CELL ADHESION MOLECULE; N ACETYLGLUCOSAMINE; PRESPORE SPECIFIC ANTIGEN; UNCLASSIFIED DRUG;

ANTIGEN DETECTION; ANTIGEN SPECIFICITY; ARTICLE; CELL ADHESION; CYTOARCHITECTURE; DICTYOSTELIUM DISCOIDEUM; GENETIC RECOMBINATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; PROTEIN ISOLATION;

ACETYLGLUCOSAMINE; AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, PROTOZOAN; ANTIGENS, SURFACE; CARBOHYDRATE CONFORMATION; CHROMATOGRAPHY, GAS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DICTYOSTELIUM; FUNGAL PROTEINS; GLYCOPEPTIDES; GLYCOSYLATION; MAGNETIC RESONANCE SPECTROSCOPY; MASS SPECTROMETRY; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, SECONDARY; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TRYPSIN;

EID: 0029894645     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0511z.x     Document Type: Article

References (42)

1. Alexander, S., Smith, E., Davis, L., Gooley, A. A., Por, S. B., Browne, L. & Williams, K. L. (1988) Characterisation of an antigenically related family of cell-type specific proteins implicated in slug migration in Dictyostelium discoideum, Differentiation 38, 82-90.
2. Ames, B. W. (1966) Assay of inorganic phosphate, total phosphate and phosphatases, Methods Enzymol. 8, 115-118.
3. Barclay, A. N., Birkeland, M. L. Brown, M. H., Beyers, A. D., Davis, S. J., Samoza, C. & Williams, A. F. (1993) The leucocyte antigen facts book, 1st edn, Academic Press Inc., London.
4. Barklis, E. & Lodish, H. F. (1983) Regulation of Dictyostelium discoideum mRNAs specific for prespore and prestalk cells, Cell 32, 1134-1148.
5. 31P NMR reference standards for aqueous samples, J. Magn. Reson. 49, 172-174.
6. Browne, L. H. & Williams, K. L. (1993) Gradients in the expression of cell surface glycoproteins in a simple tissue, the Dictyostelium discoideum slug, J. Gen. Microbiol. 139, 847-853.
7. Clarke, A. J. (1993) Compositional analysis of peptidoglycan by high performance anion-exchange liquid chromatography. Anal. Biochem. 212, 244-250.
8. Dittrich, W., Williams, K. L. & Slade, M. B. (1994) Production and secretion of recombinant proteins in Dictyostelium discoideum. Biotechnology 12, 614-618.
9. Dong, D. L.-Y., Xu, Z.-S., Chevrier, M. R., Cotter, R. J., Cleveland, D. W. & Hart, G. W. (1993) Glycosylation of mammalian neurofilaments, J. Biol. Chem. 268, 16679-16687.
10. Dong, D. L.-Y. & Hart, G. W. (1994) Purification and characterisation of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol, J. Biol. Chem. 269, 19321-19330.
11. Early, A. E., Williams, J. G., Meyer, H. E., Por, S. B., Smith, E. & Williams, K. L. (1988) Structural characterisation of the Dictyostelium discoideum prespore-specific gene D19 and of its product, the cell surface glycoprotein PsA, Mol. Cell. Biol. 8, 3458-3466.
12. Gooley, A. A., Classon, B. J., Marshchalek, R. & Williams, K. L. (1991) Glycosylation sites identified by detection of glycosylated amino acids released from Edman degradation: the identification of Xaa-Pro-Xaa-Xaa as a motif for the Thr-O-glycosylation, Biochem. Biophys. Res. Commun. 178, 1194-1201.
13. Gooley, A. A., Marshchalek, K. & Williams, K. L. (1992) Size polymorphisms due to changes in the number of O-glycosylated tandem repeats in the Dictyostelium discoideum glycoprotein PsA, Genetics 130, 749-756.
14. Gooley, A. A. & Williams, K. L. (1994) Towards characterising O-glycans: the relative merits of in vivo and in vitro approaches in seeking peptide motifs specifying O-glycosylation sites, Glycobiol. 4, 413-417.
15. Gooley, A. A., Packer, N. H., Pisano, A., Redmond, J. W., Williams, K. L., Jones, A., Loughnan, M. & Alewood, P. F. (1995) Characterization of individual N- and O-linked glycosylation sites using Edman degradation, in Techniques in protein chemistry VI (Crabb, J. W., ed.) pp. 83-90, Academic Press, Inc., San Diego.
16. Greis, K. D., Gibson, W. & Hart, G. W. (1994) Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952, J. Virol. 68, 8339-8349.
17. Haltiwanger, R. S., Kelly, W. G., Roquemore, E. P., Blomberg, M. A., Dong, D. L.-Y., Kreppel, L., Chou, T.-Y. & Hart, G. W. (1992) Glycosylation of nuclear and cytoplasmic proteins is ubiquitous and dynamic, Biochem. Soc. Trans. 20, 264-269.
18. Hart, G. W., Haltiwanger, R. S., Holt, G. D. & Kelly, W. G. (1989) Glycosylation in the nucleus and cytoplasm, Annu. Rev. Biochem. 58, 841-874.
19. Haynes, P. A., Gooley, A. A., Ferguson, M. A. J., Redmond, J. W. & Williams, K. L. (1993) Post-translational modifications of the Dictyostelium discoideum glycoprotein PsA. Glycosylphosphatidylinositol membrane anchor and composition of O-linked oligosaccharides, Eur. J. Biochem. 216, 729-737.
20. Hill, C. P., Yee, J., Selested, M. E. & Eisenberg, D. (1991) Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane premeabilisation, Science 251, 1481-1485.
21. Hohmann, H.-P. Bozzaro, S., Yoshida, M., Merkl, R. & Gerisch, G. (1987a) Two-step glycosylation of the contact site A protein of Dictyostelium discoideum and transport of an incompletely glycosylated form to the cell surface, J. Biol. Chem. 262, 16618-16624.
22. Hohmann, H.-P., Bozzaro, S., Merkl, R., Wallraff, E., Yoshida, M., Weinhart, U. & Gerisch, G. (1987b) Post-translational glycosylation of the contact site A protein of Dictyostelium discoideum is important for stability but not for its function in cell adhesion. EMBO J. 12, 3663-3671.
23. Iwata, S., Ostermeier, C., Ludwig, B. & Michael, H. (1995) Structure at 2.8 Å resolution ot cytochrome c oxidase from Paracoccus denitrificans. Nature 376, 660-669.
24. Kabsch, W. & Sander, C. (1984) On the use of sequence homology to predict protein structure: identical pentapeptides can have completely different conformations. Proc. Natl Acad. Sci. USA 81, 1075-1078.
25. Ku, N.-O. & Omary, M. B. (1994) Expression, glycosylation, and phosphorylation of human keratins 8 and 18 in insect cells. Exp. Cell Res. 211, 24-35.
26. 1H spin-spin coupling constants in proteins, Biochem. Biophys. Res. Commun. 113, 967-974.
27. Pearson, W. R. & Lipman, D. J. (1988) Improved tools for biological sequence analysis comparison, Proc. Natl Acad. Sci. USA 85, 2444-2448.
28. Piotto, M. Saudek, V. & Sklenár, V. (1992) Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions, J. Biomol. NMR 2, 661-665.
29. Previato, J. O., Jones, C., Goncalves, L. P. B., Wait, R., Travassos, L. R. & Mendonca-Previato, L. (1994) O-glycosidically linked N-acetylglucosamine-bound oligosaccharides from glycoproteins of Trypanosoma cruzi, Biochem. J. 301, 151-159.
30. Previato, J. O., Jones, C., Xavier, M. T., Wait, R., Travassos, L. R., Parodi, A. J. & Mendonca-Previato, L. (1995) Structural characterisation of the major glycosylphosphatidylinositol membrane-anchored glycoprotein from emastigote forms of Trypanosoma cruzi Y-strain, J. Biol. Chem. 270, 7241-7250.
31. Reason, A. J., Morris, H. R., Pancio, M., Marais, R., Treisam, R. H., Haltiwanger, R. S., Hart, G. W., Kelly, W. G. & Dell, A. (1992) Localisation of O-GlcNAc modification on the serum response transcription factor. J. Biol. Chem. 267, 16911-16921.
32. Richardson, J. S. (1981) The anatomy and taxonomy of protein structure, Adv. Protein Chem. 34, 167-339.
33. Sharon, N. (1975) Complex carbohydrates, their chemistry, biosynthesis and function, 1st ed, pp. 56-59, Addison-Wesley Publishing Company, London.
34. Siu, C.-H. (1990) Cell adhesion molecule's in Dictyostelium discoideum, Bioessays 12, 357-360.
35. Starr, C. M., D'Onofrio, M. Park, M. K. & Hanover, J. A. (1990) Primary sequence and heterologous expression of a nuclear pore glycoprotein p62, J. Cell Biol. 110, 1861-1871.
36. Stuart, D. L., Acharya, K. R., Walker, N. P C., Smith, S. G., Lewis, M. & Philips, D. C. (1986) Alpha lactalbumin possesses a novel calcium binding loop, Nature 324, 84-87.
37. Tarr, G. E. (1986) Manual Edman sequencing system, in Methods of protein microcharacterisation (Shivley, J. E., ed.) 1st edn, pp. 162-163, Humana Press, Clifton.
38. Wagner, G. & Wyss, D. F. (1994) Cell surface adhesion receptors, Curr. Opin. Struct. Biol. 4, 841-851.
39. Wishart, D. S., Sykes, B. D. & Richards, F. M. (1991) Relationship between nuclear magnetic resonance chemical shift and protein secondary structure, J. Mol. Biol 222, 311-333.
40. West, C. M. & Loomis, W. L. (1985) Absence of a carbohydrate modification does not attect the level or sub-cellular localisation of three membrane glycoproteins in modB mutants of Dictyostelium dis coideum, J. Biol. Chem. 260, 13803-13809.
41. Yoshida, M., Stadler, J., Bertholdt, G. & Gerisch, G. (1984) Wheat germ agglutinin binds to the contact site A glycoprotein of Dictyostelium discoideum & inhibits EDTA-stable cell adhesion, EMBO J. 3, 2663-2670.
42. Zhou-Chou, T., Slade, M. B., Williams, K. L. & Gooley, A. A. (1995) Expression, purification and characterisation of a secreted recombinant glycoprotein PsA in Dictyostelium discoideum, J. Biotechnol. 38, 137-149.