Effective amplification of long targets from cloned inserts and human genomic DNA

Proceedings of the National Academy of Sciences of the United States of America

Volumn 91, Issue 12, 1994, Pages 5695-5699

  Cheng, Suzanne ^a   Fockler, Carita ^a   Barnes, Wayne M ^b   Higuchi, Russell ^a  

^a Roche Molecular Systems Inc ^*  (Canada)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

cosolvents; genome mapping; PCR; thermostable 3' to 5' exonuclease

Indexed keywords

DIMETHYL SULFOXIDE; DNA POLYMERASE; EXONUCLEASE; GLYCEROL;

ARTICLE; BACTERIOPHAGE; CELL PH; DNA DENATURATION; DNA SEQUENCE; GENE AMPLIFICATION; GENE CLUSTER; GENE INSERTION; GENE MAPPING; GENE TARGETING; HUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; THERMOSTABILITY;

BACTERIOPHAGE LAMBDA; BUFFERS; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; EXONUCLEASES; GLOBINS; HUMAN; MOLECULAR WEIGHT; OSMOLAR CONCENTRATION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SOLVENTS; SPECIES SPECIFICITY; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC;

BACTERIOPHAGE LAMBDA;

EID: 0028261194     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.91.12.5695     Document Type: Article

References (41)