Mechanism-based active site modification of oxidosqualene cyclase by tritium-labeled 18-thia-2,3-oxidosqualene

Journal of the American Chemical Society

Volumn 118, Issue 38, 1996, Pages 9180-9181

  Abe, Ikuro ^a,b   Liu, Wei ^c   Oehlschlager, Allan C ^c   Prestwich, Glenn D ^a,b  

^a UNIVERSITY OF UTAH   (United States)

^b STONY BROOK UNIVERSITY   (United States)

^c SIMON FRASER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; LANOSTEROL SYNTHASE; SQUALENE DERIVATIVE; TRITIUM;

ARTICLE; CYCLIZATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME MODIFICATION; GEL ELECTROPHORESIS; IN VITRO STUDY; ISOTOPE LABELING; NONHUMAN; RAT; SWINE;

EID: 0029801927     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja961643a     Document Type: Article

References (40)

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35. 4 and treatment with TBDMSC1 gave 9.
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37. 50 concentration, while 15 gave quantitative recovery of OSC activity. In addition, with rat OSC, nearly 100% activity was recovered for 8 as well as for 15.
38. 3H]-8 at 37°C for 1 h, a 15 μL aliquot of the assay mixture was diluted with 300 μL of buffer A, added to 50 μL of preequilibrated DEAE-Sepahacel. After incubation for 1 h at rt, the 10000g supernatant was removed, the DEAE resin was washed (5 × 1 mL of buffer A) and resuspended in 1 mL buffer, and bound radioactivity was assessed by LSC.
39. f values for 8, 1, 3, and the polar product were 0.39, 0.48, 0.26, and 0.06, respectively. As a control experiment, boiled-enzyme preparation was employed.
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