Analysis of a FliM-FliN flagellar switch fusion mutant of Salmonella typhimurium

Journal of Bacteriology

Volumn 178, Issue 15, 1996, Pages 4582-4589

  Kihara, May ^a   Francis, Noreen R ^b   Derosier, David J ^b   Macnab, Robert M ^a  

^a YALE UNIVERSITY   (United States)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; FLAGELLUM; GENE DELETION; GENE SEQUENCE; GENETIC ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SALMONELLA TYPHIMURIUM;

SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0029785914     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.15.4582-4589.1996     Document Type: Article

References (42)

1. Aizawa, S.-I., G. E. Dean, C. J. Jones, R. M. Macnab, and S. Yamaguchi. 1985. Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium. J. Bacteriol. 161: 836-849.
2. Barak, R., and M. Eisenbach. 1992. Correlation between phosphorylation of the chemotaxis protein Che Y and its activity at the flagellar motor. Biochemistry 31: 1821-1826.
3. Bourret, R. B., J. F. Hess, and M. I. Simon. 1990. Conserved aspartate residues and phosphorylation in signal transduction by the chemotaxis protein CheY. Proc. Natl. Acad. Sci. USA 87: 41-45.
4. 3a.Blair, D. Personal communication.
5. Chun, S. Y., and J. S. Parkinson. 1988. Bacterial motility: membrane topology of the Escherichia coli MotB protein. Science 239: 276-278.
6. Dean, G. E., R. M. Macnab, J. Stader, P. Matsumura, and C. Burks. 1984. Gene sequence and predicted amino acid sequence of the MotA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. J. Bacteriol. 159: 991-999.
7. De Mot, R., and J. Vanderleyden. 1994. The C-terminal sequence conservation between OmpA-related outer membrane proteins and MotB suggests a common function in both Gram-positive and Gram-negative bacteria, possibly in the interaction of these domains with peptidoglycan. Mol. Microbiol. 12: 333-334.
8. Francis, N. R., V. M. Irikura, S. Yamaguchi, D. J. DeRosier, and R. M. Macnab. 1992. Localization of the Salmonella typhimurium flagellar switch protein FliG to the cytoplasmic M-ring face of the basal body. Proc. Natl. Acad. Sci. USA 89: 6304-6308.
9. Francis, N. R., G. E. Sosinsky, D. Thomas, and D. J. DeRosier. 1994. Isolation, characterization and structure of bacterial flagellar motors containing the switch complex. J. Mol. Biol. 235: 1261-1270.
10. Homma, M., T. Iino, and R. M. Macnab. 1988. Identification and characterization of the products of six region III flagellar genes (flaAII.3 through flaQII) of Salmonella typhimurium. J. Bacteriol. 170: 2221-2228.
11. Homma, M., K. Kutsukake, and T. Iino. 1985. Structural genes for flagellar hook-associated proteins in Salmonella typhimurium. J. Bacteriol. 163: 464-471.
12. Irikura, V. M., M. Kihara, S. Yamaguchi, H. Sockett, and R. M. Macnab. 1993. Salmonella typhimurium fliG and fliN mutations causing defects in assembly, rotation, and switching of the flagellar motor. J. Bacteriol. 175: 802-810.
13. Katayama, E., T. Shiraishi, K. Oosawa, N. Baba, and S.-I. Aizawa. 1996. Geometry of the flagellar motor in the cytoplasmic membrane of Salmonella typhimurium as determined by stereo-photogrammetry of quick-freeze deepetch replica images. J. Mol. Biol. 255: 458-475.
14. Khan, S., M. Dapice, and T. S. Reese. 1988. Effects of mot gene expression on the structure of the flagellar motor. J. Mol. Biol. 202: 575-584.
15. Kihara, M., M. Homma, K. Kutsukake, and R. M. Macnab. 1989. Flagellar switch of Salmonella typhimurium: gene sequences and deduced protein sequences. J. Bacteriol 171: 3247-3257.
16. Koebnik, R. 1995. Proposal for a peptidoglycan-associating alpha-helical motif in the C-terminal regions of some bacterial cell-surface proteins. Mol. Microbiol. 16: 1269-1270.
17. Kubori, T., N. Shimamoto, S. Yamaguchi, K. Namba, and S.-I. Aizawa. 1992. Morphological pathway of flagellar assembly in Salmonella typhimurium. J. Mol. Biol. 226: 433-446.
18. Kuo, S. C., and D. E. Koshland, Jr. 1987. Roles of cheY and cHeZ gene products in controlling flagellar rotation in bacterial chemotaxis of Escherichia coli. J. Bacteriol. 169: 1307-1314.
19. Lloyd, S. A., H. Tang, X. Wang, S. Billings, and D. F. Blair. 1996. Torque generation in the flagellar motor of Escherichia coli: evidence of a direct role for FliG but not FliM or FliN. J. Bacteriol. 178: 223-231.
20. Lukat, G. S., A. M. Stock, and J. B. Stock. 1990. Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis. Biochemistry 29: 5436-5442.
21. Macnab, R. M. 1976. Examination of bacterial flagellation by dark-field microscopy. J. Clin. Microbiol. 4: 258-265.
22. Macnab, R. M. 1995. Flagellar switch, p. 181-199. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
23. Macnab, R. M. 1996. Flagella and motility, p. 123-145. In F. C. Neidhardt, R. Curtiss III, C. A. Gross, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
24. Malakooti, J., Y. Komeda, and P. Matsumura. 1989. DNA sequence analysis, gene product identification, and localization of flagellar motor components of Escherichia coli. J. Bacteriol. 171: 2728-2734.
25. Patterson-Delafield, J., R. J. Martinez, B. A. D. Stocker, and S. Yamaguchi. 1973. A new fla gene in Salmonella typhimurium-flaR-and its mutant phenotype-superhooks. Arch. Mikrobiol. 90: 107-120.
26. Roman, S. J., M. Meyers, K. Volz, and P. Matsumura. 1992. A chemotactic signaling surface on CheY defined by suppressors of flagellar switch mutations. J. Bacteriol. 174: 6247-6255.
27. Ryu, J., and R. J. Hartin. 1990. Quick transformation in Salmonella typhimurium LT2. BioTechniques 8: 43-44.
28. Sockett, H., S. Yamaguchi, M. Kihara, V. M. Irikura, and R. M. Macnab. 1992. Molecular analysis of the flagellar switch protein FliM of Salmonella typhimurium. J. Bacteriol. 174: 793-806.
29. Stader, J., P. Matsumura, D. Vacante, G. E. Dean, and R. M. Macnab. 1986. Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. J. Bacteriol. 166: 244-252.
30. Tang, H., S. Billings, X. Wang, L. Sharp, and D. F. Blair. 1995. Regulated underexpression and overexpression of the FliN protein of Escherichia coli and evidence for an interaction between FliN and FliM in the flagellar motor. J. Bacteriol. 177: 3496-3503.
31. Tang, H., and D. F. Blair. 1995. Regulated underexpression of the FliM protein of Escherichia coli and evidence for a location in the flagellar motor distinct from the MotA/MotB torque generators. J. Bacteriol. 177: 3485-3495.
32. Welch, M., K. Oosawa, S.-I. Aizawa, and M. Eisenbach. 1993. Phosphorylation-dependent binding of a signal molecule to the flagellar switch of bacteria. Proc. Natl. Acad. Sci. USA 90: 8787-8791.
33. 2+, and conformation of the chemotaxis protein ChcY on its binding to the flagellar switch protein FliM. Biochemistry 33: 10470-10476.
34. 32a.Williams, T., and R. Macnab. Unpublished data.
35. 32b.Yamaguchi, S. Personal communication.
36. Yamaguchi, S., S.-I. Aizawa, M. Kihara, M. Isomura, C. J. Jones, and R. M. Macnab. 1986. Genetic evidence for a switching and energy-transducing complex in the flagellar motor of Salmonella typhimurium. J. Bacteriol. 168: 1172-1179.
37. Yamaguchi, S., H. Fujita, A. Ishihara, S.-I. Aizawa, and R. M. Macnab. 1986. Subdivision of flagellar genes of Salmonella typhimurium into regions responsible for assembly, rotation, and switching. J. Bacteriol. 166: 187-193.
38. Yamaguchi, S., H. Fujita, K. Sugata, T. Taira, and T. Iino. 1984. Genetic analysis of H2, the structural gene for phase-2 flagellin in Salmonella. J. Gen. Microbiol. 130: 255-265.
39. Yamaguchi, S., H. Fujita, T. Taira, K. Kutsukake, M. Homma, and T. Iino. 1984. Genetic analysis of three additional fla genes in Salmonella typhimurium. J. Gen. Microbiol. 130: 3339-3342.
40. Zhao, R., N. Pathak, H. Jane, T. S. Reese, and S. Khan. FliN is a major component of the C-ring in the Salmonella typhimurium flagellar basal body. J. Mol. Biol., in press.
41. Zhao, R., S. C. Schuster, and S. Khan. 1995. Structural effects of mutations in Salmonella typhimurium flagellar switch complex. J. Mol. Biol. 251: 400-412.
42. Zhou, J., R. T. Fazzio, and D. F. Blair. 1995. Membrane topology of the MotA protein of Escherichia coli. J. Mol. Biol. 251: 237-242.