A thermophilic alcohol dehydrogenase from Bacillus acidocaldarius not reactive towards ketones

Journal of Biochemistry

Volumn 120, Issue 3, 1996, Pages 498-504

  D'Auria, Sabato ^a   La Cara, Francesco ^a   Nazzaro, Filomena ^a   Vespa, Nunzia ^a   Rossi, Mose' ^a  

^a CNR   (Italy)

Author keywords

Activity; Alcohol dehydrogenase; Bacillus acidocaldarius; Circular dichroism; Enzymatic; Thermophilic enzymes

Indexed keywords

ALCOHOL; ALCOHOL DEHYDROGENASE; ANISALDEHYDE; BACTERIAL ENZYME; KETONE; OXIDOREDUCTASE; ZINC;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS; CIRCULAR DICHROISM; DENSITY GRADIENT CENTRIFUGATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBUNIT; GEL FILTRATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN QUATERNARY STRUCTURE; SEQUENCE HOMOLOGY; THERMOPHILIC BACTERIUM; THERMOSTABILITY;

ALICYCLOBACILLUS ACIDOCALDARIUS; ANIMALIA; BACTERIA (MICROORGANISMS); FELIS CATUS;

EID: 0029783386     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021441     Document Type: Article

References (43)

1. Branden, C.I., Jornvall, H., Eklund, H., and Furugren, B. (1975) Alcohol dehydrogenases in The Enzymes (Boyer, P.D., ed.) Vol. 11, pp. 103-190, Academic Press, New York
2. Brunger, A.T., Kuriyan, J., and Karplus, M. (1987) Crystallo-graphic R factor refinement by molecular dynamics. Science 235, 458-460
3. Brunger, A.T. (1993) Assessment of phase accuracy by cross validation: The free R value, methods and applications. Acta Crystallogr. D 49, 24-36
4. Al-Karadaghi, S., Cedergren-Zeppezauer, E.S., Hovmoller, S., Petratos, K., Terry, H., and Wilson, K.S. (1994) Refined crystal structures of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution. Acta Crystallogr. D 50, 793-807
5. Sheehan, M.C., Bailey, C.J., Dowds, B.C.A., and Mc Connell, D.J. (1988) A new alcohol dehydrogenase reactive towards methanol from Bacillus stearothermophilus. Biochem. J. 252, 661-666
6. Bryant, R.O., Wiegel, J., and Ljungdahl, L.G. (1988) Purification and properties of primary and secondary alcohol dehydrogenases from Thermoanaerobacter ethanolicus. Appl. Environ. Microbiol. 54, 460-465
7. Peretz, M. and Burstein, Y. (1989) Amino acid sequence of alcohol dehydrogenase from the thermophilic bacterium Thermoanaerobium brockii. Biochemistry 28, 6549-6555
8. Keinan, E., Hafeli, E.K., Seth, K.K., and Lamed, R. (1986) Thermostable enzymes in organic synthesis. Asymmetric reduction of ketones with alcohol dehydrogenase from Thermoanaerobium brockii. J. Am. Chem. Soc. 108, 162-169
9. + dependent alcohol dehydrogenase from Sulfolobus solfataricus: Gene and protein sequence determination and relationship to other alcohol dehydrogenases. Biochemistry 31, 12514-12523
10. Millonig, G., De Rosa, M., Gambacorta, A., and Bu'Lock, J.D. (1975) Ultrastructure of an extremely thermophilic acidophilic microorganism. J. Gen. Microbiol. 86, 165-173
11. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of Protein-dye binding. Anal. Biochem. 72, 248-254
12. Martin, R.G. and Ames, B.N. (1961) A method for determining the sedimentation behaviour of enzymes: Application to protein mixtures. J. Biol. Chem. 236, 1372-1379
13. Andrews, P. (1964) Estimation of the molecular weights of proteins by Sephadex gel-filtration. Biochem. J. 91, 222-223
14. 4. Nature 227, 680-685
15. Robertson, E.F., Dannely, H.K., Mallay, P.J., and Reeves, H.C. (1987) Rapid isoelectric focusing in a vertical polyacrylamide minigel system. Anal. Biochem. 167, 290-294
16. Rudge, J. and Bickerstaff, G.F. (1986) Purification and properties of an alcohol dehydrogenase from Sporotrichum pulverulentum. Enzyme Microb. Technol. 8, 120-124
17. Young, J.T., Wu, C.-S.C., and Martinez, H.M. (1986) Calculation of protein conformation from circular dichroism. Methods Enzymol. 130, 208-269
18. Jornvall, H., Persson, M., and Jeffery, J. (1987) Characteristics of alcohol/polyol dehydrogenases. Eur. J. Biochem. 167, 195-201
19. Lamed, R.J. and Zeikus, J.G. (1981) Novel NADP-linked alcohol-aldehyde/ketone oxidoreductase in thermophilic ethanologenic bacteria. Biochem. J. 195, 183-190
20. Lamed, R.J. and Zeikus, J.G. (1980) Ethanol production by thermophilic bacteria: Relationship between fermentation product yields of and catabolic enzyme activities in Clostridium thermocellum and Thermoanaerobium brokii. J. Bacterial. 144, 569-578
21. Guagliardi, A., Martino, M., Iaccarino, I., De Rosa, M., Rossi, M., and Bartolucci, S. (1996) Purification and characterization of the alcohol dehydrogenase from a novel strain of Bacillus stearothermophilus growing at 70°C. Int. J. Biochem. Cell. Biol. 28, 239-246
22. Roy, S.K. and Nishikawa, A.H. (1979) Large-scale isolation of equine liver alcohol dehydrogenase on a Blue agarose gel. Biotechnol. Bioeng. 21, 775-785
23. Persson, M., Krook, M., and Jornvall, H. (1991) Characteristics of short-chain alcohol dehydrogenases and related proteins. Eur. J. Biochem. 200, 537-543
24. Goodlove, P.E., Cunningham, P.R., Parker, J., and Clark, D.P. (1989) Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli. Gene 85, 209-214
25. Llewellyn, D.J., Finnegan, E.J., Ellis, J.G., Dennis, E.S., and Peacock, W.J. (1987) Structure and expression of an alcohol dehydrogenase 1 gene from Pisum sativum (cv. "Greenfast"). J. Mol. Biol. 195, 115-123
26. Eklund, H., Branden, C., and Jornvall, H. (1976) Structural comparison of mammalian, yeast and bacillar alcohol dehydrogenases. J. Mol. Biol. 102, 61-73
27. Raia, C.A., D'Auria, S., and Rossi, M. (1994) NAD-dependent alcohol dehydrogenase from Sulfolobus solfataricus: Structural and functional features. Biocatalysis 11, 143-150
28. Cannio, R., Fiorentino, G., Carpinelli, P., Rosai, M., and Bartolucci, S. (1996) Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species. J. Bacterial. 178, 301-305
29. Jornvall, H., Eklund, H., and Branden, C.I. (1978) Subunit conformation of yeast alcohol dehydrogenase. J. Biol. Chem. 253, 8414-8419
30. Bartolucci, S., Rella, R., Guagliardi, A., Raia, C.A., Gambacorta, A., De Rosa, M., and Rossi, M. (1987) Malic enzyme from archaebacterium Sulfolobus solfataricus. J. Biol. Chem. 262, 7725-7731
31. Pisani, F.M., Rella, R., Rozzo, C., Raia, C.A., Nucci, R., Gambacorta, A., De Rosa, M., and Rossi, M. (1990) Thermostable β-galactosidase from the archaebacterium Sulfolobus solfataricus. Purification and properties. Eur. J. Biochem. 187, 321-328
32. Rella, R., Raia, C.A., Pensa, M., Pisani, P.M., Gambacorta, A., De Rosa, M., and Rossi, M. (1987) A novel archaebacterial NAD-dependent alcohol dehydrogenase. Purification and properties. Eur. J. Biochem. 167, 475-479
33. Wrba, A., Schweiger, A., Schultes, V., and Jaenicke, R. (1990) Extremely thermostable D-glyceraldehyde-3-phosphate dehydrogenase from the eubacterium Thermotoga marittima. Biochemistry 29, 7584-7592
34. Raia, C.A., D'Auria, S., Guagliardi, A., Bartolucci, S., De Rosa, M., and Rossi, M. (1995) Characterization of redox proteins from extreme thermophilic archaebacteria: Studies on alcohol dehydrogenase and thioredoxins. Biosensor Bioelectron. 10, 135-140
35. Eklund, H., Jones, A., and Schneider, G. (1986) Active site in alcohol dehydrogenase in Zinc Enzymes (Bertini, I., ed.) Vol. 1, pp. 377-392, Birkhauser Boston, Boston
36. Ribas de Pouplana, L., Atrian, S., Gonzales-Duarte, R., Fothergill-Gilmore, L.A., Kelly, S.M., and Price, N.C. (1991) Structural properties of long- and short-chain alcohol dehydrogenase. Biochem. J. 276, 433-438
37. Colbear, T., Daniel, R.M., and Morgan, H.W. (1992) The enzymes from extreme thermophiles: Bacterial sources, thermostabilities and industrial relevance. Adv. Biochem. Eng. Biotechnol. 45, 57-98
38. Simpson, H.D. and Cowan, D.A. (1992) Secondary alcohol dehydrogenases from extremely thermophilic bacteria. Ann. N.Y. Acad. Sci. 672, 145-151
39. Adolph, H.W., Maurer, P., Schneider-Bernlohr, H., Sartorius, C., and Zeppezauer, M. (1991) Substrate specificity and stereo-selectivity of horse liver alcohol dehydrogenase. Eur. J. Biochem. 201, 615-625
40. Dalziel, K. and Dickinson, F.M. (1966) Substrate activation and inhibition in coenzyme-substrate reactions cycloexanol oxidation catalyzed by liver alcohol dehydrogenase. Biochem. J. 100, 491-500
41. Ramaswamy, S., El Ahmad, M., Danielsson, O., Jornvall, H., and Eklud, H. (1996) Crystal structure of cod liver class I alcohol dehydrogenase: Substrate pocket and structurally variable segments. Protein Sci. 5, 663-671
42. Raia, O.A., Caruso, C., Marino, M., Vespa, N., and Rossi, M. (1996) Activation of Sulfolobus solfataricus alcohol dehydrogenase by modification of cysteine residue 38 with iodoacetic acid. Biochemistry 35, 638-647
43. Bridgen, J., Kolb, E., and Harris, J.I. (1973) Amino acid sequence homology in alcohol dehydrogenase. FEBS Lett. 33, 1-3