Aggregates of scrapie-associated prion protein induce the cell-free conversion of protease-sensitive prion protein to the protease-resistant state

Chemistry and Biology

Volumn 2, Issue 12, 1995, Pages 807-817

  Caughey, Byron ^a   Kocisko, David A ^a,b   Raymond, Gregory J ^a   Lansbury Jr , Peter T ^b  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

aggregation; in vitro formation; prion protein; scrapie; sedimentation

Indexed keywords

SCRAPIE PRION; ANIMALIA;

DIAGNOSTIC AGENT; PROTEINASE; SULFUR;

ANIMAL; ARTICLE; CELL FREE SYSTEM; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRION; SCRAPIE; ULTRACENTRIFUGATION; ULTRAFILTRATION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; KINETICS; MOLECULAR WEIGHT; PRIONS; SCRAPIE; SULFUR RADIOISOTOPES; ULTRACENTRIFUGATION; ULTRAFILTRATION;

EID: 0029583794     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/1074-5521(95)90087-X     Document Type: Article

References (47)

1. Prions and related neurological diseases
2. Identification of a protein that purifies with the scrapie prion
3. Scrapie infectivity, fibrils and low molecular weight protein
4. Scrapie prions aggregate to form amyloid-like birefringent rods
5. A cellular gene encodes scrapie PrP 27–30 protein
6. The major polypeptide of scrapie-associated fibrils (SAF) has the same size, charge distribution and N-terminal protein sequence as predicted for the normal brain protein (PrP)
7. Separation and properties of cellular and scrapie prion protein
8. Detection of scrapie-associated fibril (SAF) proteins using anti-SAF antibody in non-purified tissue preparations
9. Scrapie and cellular prion proteins differ in the kinetics of synthesis and topology in cultured cells
10. The scrap ie-associated form of PrP is made from a cell surface precursor that is both protease- and phospholipase-sensitive
11. Structural studies of the scrapie prion protein using mass spectrometry and amino acid sequencing
12. A proteaseresistant protein is a structural component of the scrapie prion
13. Congo red inhibition of scrapie agent eplication
14. Self-replication and scrapie
15. A modified host protein model of scrapie
16. Molecular biology of prion diseases
17. Cell-free formation of protease-resistant prion protein
18. Species specificity in the cell-free conversion of prion protein to protease-resistant forms: a model for the scrapie species barrier
19. Nongenetic propagation of strain-specific properties of scrapie prion protein
20. The new biology of spongiform encephalopathy: infectious amyloidoses with a genetic twist
21. Seeding ‘one-dimensional crystallization’ of amyloid: a pathogenic mechanism in Alzheimer's disease and scrapie?
22. Conversion of truncated and elongated prion proteins into the scrapie isoform in cultured cells
23. Conformational transitions, dissociation, and unfolding of scrapie amyloid (prion) protein
24. Viral particles are required for infection in neurodegenerative Creutzfeld-Jakob disease
25. Secondary structure analysis of the scrapieassociated protein PrP 27–30 in water by infrared spectroscopy
26. Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules
27. The measurement of cooperative protein self-assembly by turbidity and other techniques
28. Polymerization of flagellin and polymorphism of flagella
29. Sickle cell hemoglobin polymerization
30. One sequence, two folds: a metastable structure of CD2
31. Immunolocalization of heparan sulfate proteoglycans to the prion protein amyloid plaques of Gerstmann-Straussler syndrome, Creutzfeldt-Jakob disease and scrapie
32. Binding of the protease-sensitive form of PrP (prion protein) to sulfated glycosaminoglycan and Congo red
33. The search for scrapie agent nucleic acid
34. The search for a nucleic acid component of scrapie infectivity
35. The scrapie agent: ‘a virus by any other name’
36. Properties of scrapie prion protein liposomes
37. Scrapie prion liposomes and rods exhibit target sizes of 55,000 Da
38. Molecular mass, biochemical composition, and physicochemical behavior of the infectious form of the scrapie precursor protein monomer
39. Purified prion proteins and scrapie infectivity copartition into liposomes
40. Radiation inactivation of glutamate dehydrogenase hexamer: lack of energy transfer between subunits
41. Molecular weight determinations from radiation inactivation
42. Characterization of the insulin receptor in its membrane environment by radiation inactivation
43. Identification of glycoinositol-phospholipid-linked and truncated forms of the scrapie prion protein
44. Foreign PrP expression and scrapie infection in tissue culture cell lines
45. Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins
46. Sedimentation characteristics of the scrapie agent from murine spleen and brain
47. N-terminal truncation of the scrapie-associated form of PrP by lysosomal protease(s): implications regarding the site of conversion of PrP to the protease-resistant state