Antisense technology reveals the α(2A) adrenoceptor to be the subtype mediating the hypnotic response to the highly selective agonist, dexmedetomidine, in the locus coeruleus of the rat

Journal of Clinical Investigation

Volumn 98, Issue 5, 1996, Pages 1076-1080

  Mizobe, Toshiki ^a   Maghsoudi, Kaveh ^a   Sitwala, Kajal ^a   Tianzhi, Guo ^a   Ou, Jennie ^a   Maze, Mervyn ^a,b  

^a STANFORD UNIVERSITY   (United States)

^b VA PALO ALTO HEALTH CARE SYSTEM   (United States)

Author keywords

anesthesia; antisense; oligodeoxynucleotides, antisense; sleep; (2A) adrenergic agonists

Indexed keywords

ALPHA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; ALPHA 2A ADRENERGIC RECEPTOR; ANTISENSE OLIGODEOXYNUCLEOTIDE; DEXMEDETOMIDINE; RECEPTOR SUBTYPE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; LATENT PERIOD; LOCUS CERULEUS; NONHUMAN; PRIORITY JOURNAL; RAT; RECEPTOR AFFINITY; RIGHTING REFLEX;

EID: 0029758145     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI118887     Document Type: Article

References (31)

1. A receptor/chloride channel complex in anesthesia. Anesthesiology. 78:757-776.
2. 2 agonist is mediated in the locus coeruleus in rats. Anesthesiology. 76:948-952.
3. 2 agonist in the locus coeruleus of the rat. Pharmacol. Biochem. Behav. 43:723-727.
4. 2 agonist in the rat. J. Pharmacol. Exp. Ther. 263:1046-1049.
5. 2 adrenergic agonist dexmedetomidine in the locus coeruleus of the rat. Anesthesiology. 81:1527-1534.
6. 2-adrenoceptor agonists and anesthesia. Int. Anesthesiol. Clin. 33:81-102.
7. Ordway, G.A., S.M. Jaconetta, and A.E. Halaris. 1993. Characterization of the subtypes of alpha-2 adrenoceptors in the human brain. J. Pharmacol. Exp. Ther. 264:967-976.
8. 2-adrenergic receptor subtypes in brain. Pharmacol. Biochem. Behav. 41:267-273.
9. Nicholas, A.P., V. Pieribone, and T. Hokfelt. 1993. Distribution of mRNAs for alpha-2 adrenergic receptor subtypes in rat brain: an in situ hybridization study. J. Comp. Neurol. 328:575-594.
10. 2-adrenergic receptor subtype in human spinal cord. Anesthesiology. 77: 983-991.
11. 3H-RX821002 ligand binding. Pharmacol. Toxicol. 69:341-350.
12. Zeng, D., and K.R. Lynch. 1991. Distribution of a2-adrenergic receptor mRNA in the rat CNS. Mol. Brain Res. 10:219-225.
13. 2-adrenergic receptor genes. Hypertension (Dallas). 21:861-865.
14. 2B-adrenoceptor subtypes in postmortem human brain. J. Neurochem. 58:1555-1560.
15. Scheinin, M., J.W. Lomasney, D.M. Hayden-Hixson, U.B. Schambra, M.G. Caron, R.J. Lefkowitz, and R.T. Fremeau, Jr. 1994. Distribution of alpha 2-adrenergic receptor subtype gene expression in rat brain. Mol. Brain Res. 21: 133-149.
16. 2-adrenoceptors in the central nervous system. J. Physiol. Pharmacol. 46:241-258.
17. Standifer, K.M., C.C. Chien, C. Wahlestedt, G.P. Brown, and G.W. Pasternak. 1994. Selective loss of delta opioid analgesia and binding by antisense oligodeoxynucleotides to a delta opioid receptor. Neuron. 12:805-810.
18. Wahlestedt, C., E.M. Pich, G.F. Koob, F. Yee, and M. Heilig. 1993. Modulation of anxiety and neuropeptide Y-Y1 receptors by antisense oligonucleotides. Science (Wash. DC). 259:528-531.
19. Wahlestedt, C., E. Golanov, S. Yamamoto, F. Yee, H. Ericson, H. Yoo, C.E. Inturrisi, and D.J. Reis. 1993. Antisense oligodeoxynucleotides to NMDA-R1 receptor channel protect cortical neurons from excitotoxicity and reduce focal isehaemic infarctions. Nature (Lond.). 363:260-263.
20. Rossi, G., Y.X. Pan, J. Cheng, and G.W. Pasternak. 1994. Blockade of morphine analgesia by an antisense oligodeoxynucleotide against the mu receptor. Life Sci. 54:375-379.
21. Akabayashi, A., C. Wahlestedt, J.T. Alexander, and S.F. Leibowitz. 1994. Specific inhibition of endogenous neuropeptide Y synthesis in arcuate nucleus by antisense oligonucleotides suppresses feeding behavior and insulin secretion. Mol. Brain Res. 21:55-61.
22. Duzic, E., and S.M. Lanier. 1992. Factors determining the specificity of signal transduction by guanine nucleotide-binding protein-coupled receptors. J. Biol. Chem. 267:24045-24052.
23. Mizobe, T., M. Maze, V. Lam, S. Suryanarayana, and B. Kobilka. 1996. Arrangement of transmembrane domains in adrenergic receptors. Similarity to bacteriorhodopsin. J. Biol. Chem. 271:2387-2389.
24. Lowry, O.H., M.J. Rosebrough, A.L. Farr, and R.J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
25. 2-adrenoceptor binding and function after irreversible inactivation by n-ethoxycarbonyl-2-ethoxy-1,2-dihydroxyquinoline (EEDO). Eur. J. Pharmacol. 116:175-178.
26. Stein, C.A., and Y.C. Cheng. 1993. Antisense oligonucleotides as therapeutic agents - is the bullet really magical? Science (Wash. DC). 261:1004-1012.
27. Milligan, J.F., M.D. Mattcucci, and J.C. Martin. 1993. Current concepts in antisense drug design. J. Med. Chem. 36:1923-1937.
28. Mahan, L.C., R.M. McKernan, and P.A. Insel. 1987. Metabolism of α-and β-adrenergic receptors in vitro and in vivo. Annu. Rev. Pharmacol. Toxicol. 27:215-235.
29. 2-adrenergic agonist. Anesthesiology. In press.
30. Link, R.E., K. Desai, L. Hein, M.S. Stevens, A. Chruscinski, D. Bernstein, G.S. Barsh, and B.K. Kobilka. 1996. Cardiovascular regulation in mice lacking alpha-2-adrenoceptor subtypes B and C. Science (Wash. DC). In press.
31. Bousquet, P., J. Feldman, E. Tibirica, G. Bricca, H. Greney, M. Dontenwill, J. Stutzmann, and A. Belcourt. 1992. Imidazoline receptors: a new concept in central regulation of the arterial blood pressure. Am. J. Hypertens. 5:47S-50S.