Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide

Science

Volumn 273, Issue 5283, 1996, Pages 1853-1856

  Jabs, Thorsten ^a,c   Dietrich, Robert A ^a,b   Dangl, Jeffery L ^a,b  

^a Max Delbruck Laboratorium der Max Planck Gesellschaft   (Germany)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

REACTIVE OXYGEN METABOLITE; SUPEROXIDE;

ARABIDOPSIS; ARTICLE; CELL DEATH; GENE EXPRESSION; MUTANT; NONHUMAN; PLANT LEAF; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

EID: 0029746193     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.273.5283.1853     Document Type: Article

References (57)

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29. RNA (5 μg per lane) was isolated (10 leaves from three plants) and blots were prepared, probed, and washed as described [S. Kiedrowski, P. Kawalleck, K. Hahlbrock, I. E. Somssich, J. L. Dangl, EMBO J. 11, 4677 (1992)]. The probes used were PR-1 cDNA [S. Uknes et al., Plant Cell 5, 159 (1993)] and ESTs [T. Newman et al., Plant Physiol. 106, 1241 (1994)]. The ESTs were from the Arabidopsis Biological Resource Center, Ohio State University. EST identification numbers are as follows: B25XP, homologous to PRXcb (GenBank accession number X71794); 90B13T7, GST (GenBank accession number T20657), and a type III GST, similar to an auxin-regulated protein and GST clones [F. N. J. Droog, P. J. J. Hooykaas, B. J. van der Zaat, Plant Physiol. 107, 11139 (1995)]. Other Arabidopsis GSTs described are class I (GST2 and PMA239x14). ESTs showing no difference in expression between Isd1 and the wild type putatively encode Fe-SOD (34D9T7); Cu/ Zn-SOD (2G11T7P and 92L6T7); Mn-SOD (105G4T7); Catalase I (35F2T7, 38C1T7, and 40A1T7); anionic lignin-forming peroxide (ATTS0592); cationic peroxidase (40H1T7); P7 peroxidase (11B11T7); glutathione peroxidase (139F9T7); and lipoxygenase I (92H8T7). The apparent lack of SA-induced PR-1 mRNA accumulation in wild-type leaves (Fig. 2D) is due to deliberate underexposure of the autoradiogram in order to visualize the stronger signals. Normal exposure clearly shows PR-1 mRNA accumulation.
30. RNA (5 μg per lane) was isolated (10 leaves from three plants) and blots were prepared, probed, and washed as described [S. Kiedrowski, P. Kawalleck, K. Hahlbrock, I. E. Somssich, J. L. Dangl, EMBO J. 11, 4677 (1992)]. The probes used were PR-1 cDNA [S. Uknes et al., Plant Cell 5, 159 (1993)] and ESTs [T. Newman et al., Plant Physiol. 106, 1241 (1994)]. The ESTs were from the Arabidopsis Biological Resource Center, Ohio State University. EST identification numbers are as follows: B25XP, homologous to PRXcb (GenBank accession number X71794); 90B13T7, GST (GenBank accession number T20657), and a type III GST, similar to an auxin-regulated protein and GST clones [F. N. J. Droog, P. J. J. Hooykaas, B. J. van der Zaat, Plant Physiol. 107, 11139 (1995)]. Other Arabidopsis GSTs described are class I (GST2 and PMA239x14). ESTs showing no difference in expression between Isd1 and the wild type putatively encode Fe-SOD (34D9T7); Cu/ Zn-SOD (2G11T7P and 92L6T7); Mn-SOD (105G4T7); Catalase I (35F2T7, 38C1T7, and 40A1T7); anionic lignin-forming peroxide (ATTS0592); cationic peroxidase (40H1T7); P7 peroxidase (11B11T7); glutathione peroxidase (139F9T7); and lipoxygenase I (92H8T7). The apparent lack of SA-induced PR-1 mRNA accumulation in wild-type leaves (Fig. 2D) is due to deliberate underexposure of the autoradiogram in order to visualize the stronger signals. Normal exposure clearly shows PR-1 mRNA accumulation.
31. RNA (5 μg per lane) was isolated (10 leaves from three plants) and blots were prepared, probed, and washed as described [S. Kiedrowski, P. Kawalleck, K. Hahlbrock, I. E. Somssich, J. L. Dangl, EMBO J. 11, 4677 (1992)]. The probes used were PR-1 cDNA [S. Uknes et al., Plant Cell 5, 159 (1993)] and ESTs [T. Newman et al., Plant Physiol. 106, 1241 (1994)]. The ESTs were from the Arabidopsis Biological Resource Center, Ohio State University. EST identification numbers are as follows: B25XP, homologous to PRXcb (GenBank accession number X71794); 90B13T7, GST (GenBank accession number T20657), and a type III GST, similar to an auxin-regulated protein and GST clones [F. N. J. Droog, P. J. J. Hooykaas, B. J. van der Zaat, Plant Physiol. 107, 11139 (1995)]. Other Arabidopsis GSTs described are class I (GST2 and PMA239x14). ESTs showing no difference in expression between Isd1 and the wild type putatively encode Fe-SOD (34D9T7); Cu/ Zn-SOD (2G11T7P and 92L6T7); Mn-SOD (105G4T7); Catalase I (35F2T7, 38C1T7, and 40A1T7); anionic lignin-forming peroxide (ATTS0592); cationic peroxidase (40H1T7); P7 peroxidase (11B11T7); glutathione peroxidase (139F9T7); and lipoxygenase I (92H8T7). The apparent lack of SA-induced PR-1 mRNA accumulation in wild-type leaves (Fig. 2D) is due to deliberate underexposure of the autoradiogram in order to visualize the stronger signals. Normal exposure clearly shows PR-1 mRNA accumulation.
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57. We thank J. Ryals and S. Uknes for critical comments on the manuscript and the PR-1 cDNA, and U. Ringeisen (Köln) and S. Whitfield (Chapel Hill) for help in preparing figures. Supported by grants from the German Research Society's (Deutsche Forschungsgeimenschaft) "Arabidopsis" Focus Program (to J.L.D.) and the EEC-BIOTECH Project of Technological Priority (to J.L.D.) and by a Max-Planck-Society fellowship to T.J.