Control of doublesex alternative splicing by transformer and transformer-2 in Drosophila

Science

Volumn 252, Issue 5007, 1991, Pages 833-836

  Hoshijima, Kazuyuki ^a   Inoue, Kunio ^a   Higuchi, Ikuko ^a   Sakamoto, Hiroshi ^a   Shimura, Yoshiro ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DROSOPHILA MELANOGASTER; MELANOGASTER; ANIMALIA;

MESSENGER RNA;

ANIMAL EXPERIMENT; ARTICLE; DROSOPHILA; EMBRYO; FEMALE; MALE; NONHUMAN; PRIORITY JOURNAL; SEX DIFFERENTIATION;

ANIMAL; BASE SEQUENCE; CHROMOSOME MAPPING; DROSOPHILA MELANOGASTER; GENE EXPRESSION REGULATION; GENES, REGULATOR; GENES, STRUCTURAL; MOLECULAR SEQUENCE DATA; REPETITIVE SEQUENCES, NUCLEIC ACID; RNA SPLICING; RNA, MESSENGER; SEX DIFFERENTIATION; SUPPORT, NON-U.S. GOV'T; TRANSFECTION;

EID: 0025815671     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1902987     Document Type: Article

References (29)

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19. The region from and including the entire third exon to the Pvu II site located 1284 bp downstream of the female acceptor site, and the region from the Ace I site located 250 bp upstream of the male acceptor site to the site located 64 bp downstream of the donor site of the fifth exon, were joined and inserted into the copia vector.
20. M. McKeown, J. M. Belote, R. T. Boggs, Cell 53, 887 (1988).
21. K. Hoshijima et al., unpublished observations.
22. R. N. Nagoshi and B. S. Baker, Genes Dev. 4, 89 (1990).
23. The dsx fragment that extends from the third exon to 1128 bp downstream of the female acceptor site and which excludes the polyadenylation signal (Af) was fused to the ftz fragment that contains a portion of the intron and the following second exon {corresponding to bases 825 to 2534 of the published sequence [A. Laughon and M. P. Scott, Nature 310, 25 (1984)]}. The resulting fragment was inserted into the copia vector.
24. The region from the third exon (138 bp) to the site 48 bp downstream of the donor site of the third exon, and the region from the site 250 bp upstream of the male acceptor site to the site 64 bp downstream of the donor site of the fifth exon, were joined and inserted into the copia vector.
25. The copia-dsx deletion mutants were constructed as follows: the regions between 345 to 460 bp (R1-5-6), 234 to 459 bp (R5-6), 190 to 480 bp (R6), 344 to 599 bp (R1), or 234 to 599 bp (RO) downstream of the female acceptor site were deleted from copia-dsx, and Kpn I linkers were inserted. A polymerase chain reaction (PCR) fragment that contained the region 268 to 627 bp downstream of the female-specific acceptor site was inserted at the Kpn I linker of the RO construct in the correct orientation (SR) or in the opposite orientation (ASR). PCR was performed essentially as described [R. K. Saiki et al., Science 239, 487 (1988)].
26. M. R. Green, Annu. Rev. Genet. 20, 671 (1986).
27. Oligonucleotide-directed mutagenesis was performed essentially as described [M. J. Zoller and M. Smith, Methods Enzymol. 100, 468 (1983)].
28. The region 340 to 1128 bp downstream of the female acceptor site was deleted from copia-dtz, copia-dtz(Py18), and copia-drz(Py13). Pre-mRNA expressed from the deletion construct of copia-dtz was spliced at the ftz acceptor site even in the presence of the tra and tra-2 products in Kc cells.
29. We thank M. McKeown for tra cDNA and H. Amrein for tra-2 cDNA. Supported by Grant-in-Aid 62065009 for specially promoted research from the Ministry of Education, Science and Culture of Japan.