Endogenous tryptophan residues of cAPK regulatory subunit type IIβ reveal local variations in environments and dynamics

Proteins: Structure, Function and Genetics

Volumn 51, Issue 4, 2003, Pages 552-561

  Zawadzki, Kerri M ^a   Pan, Chia Pin ^b   Barkley, Mary D ^b   Johnson, David ^c   Taylor, Susan S ^a,d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d NONE   (United States)

Author keywords

cAPK; Fluorescence; Protein kinase A; Regulatory sub unit; Tryptophan

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; TRYPTOPHAN;

ANISOTROPY; ARTICLE; CIRCULAR DICHROISM; DIMERIZATION; FLUORESCENCE; POINT MUTATION; PRIORITY JOURNAL; QUANTUM YIELD; SOLVATION; STEADY STATE;

AMINO ACID SEQUENCE; BINDING SITES; CIRCULAR DICHROISM; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME STABILITY; FLUORESCENCE POLARIZATION; KINETICS; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; TRYPTOPHAN; VARIATION (GENETICS);

EID: 0012580091     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.10326     Document Type: Article

References (41)

1. Ogreid D, Døskeland SO. The kinetics of the interaction between cyclic AMP and the regulatory moiety of protein kinase II. Evidence for interaction between the binding sites for cyclic AMP. FEBS Lett 1981;129:282-286.
2. Corbin JD, Keely SL, Park CR. The distribution and dissociation of cyclic adenosine 3′:5′-monophosphate-dependent protein kinases in adipose, cardiac, and other tissues. J Biol Chem 1975;250:218-225.
3. De Camilli P, Moretti M, Donini SD, Walter U, Lohmann SM. Heterogeneous distribution of the cAMP receptor protein RII in the nervous system: evidence for its intracellular accumulation on microtubules, microtubule-organizing centers, and in the area of the golgi complex. J Cell Biol 1986;103:189-203.
4. Rubin CS, Rangel-Aldao R, Sarkar D, Erlichman J, Fleischer N. Characterization and comparison of membrane-associated and cytosolic cAMP-dependent protein kinases. Physicochemical and immunological studies on bovine cerebral cortex protein kinases. J Biol Chem 1979;254:3797-3805.
5. Hofmann F, Beavo JA, Bechtel PJ, Krebs EG. Comparison of adenosine 3′:5′-monophosphate-dependent protein kinases from rabbit skeletal and bovine heart muscle. J Biol Chem 1975;250:7795-7801.
6. Scott JD, Glaccum MB, Zoller MJ, Uhler MD, Helfman DM, McKnight GS, Krebs EG. The molecular cloning of a type II regulatory subunit of the cAMP-dependent protein kinase from rat skeletal muscle and mouse brain. Proc Natl Acad Sci USA 1987;84:5192-5196.
7. Cummings DE, Brandon EP, Planas JV, Motamed K, Idzerda RL, McKnight GS. Genetically lean mice result from targeted disruption of the RII beta subunit of protein kinase A. Nature 1996;382:622-626.
8. Amieux PS, Cummings DE, Motamed K, Brandon EP, Wailes LA, Le K, Idzerda RL, McKnight GS. Compensatory regulation of RIalpha protein levels in protein kinase A mutant mice. J Biol Chem 1997;272:3993-3998.
9. Brandon EP, Idzerda RL, McKnight GS. PKA isoforms, neural pathways, and behaviour: Making the connection. Curr Opin Neurobiol 1997;7:397-403.
10. Thiele TE, Willis B, Stadler J, Reynolds JG, Bernstein IL, McKnight GS. High ethanol consupmtion and low sensitivity to ethanol-induced sedation in protein kinase A-mutant mice. J Neurosci 2000;20:RC75.
11. Planas JV, Cummings DE, Idzerda RL, McKnight GS. Mutation of the RIIbeta subunit of protein kinase a differentially affects lipolysis but not gene induction in white adipose tissue. J Biol Chem 1999;274:36281-36287.
12. Su Y, Dostmann WR, Herberg FW, Durick K, Xuong NH, Ten Eyck L, Taylor SS, Varughese KI. Regulatory subunit of protein kinase A: structure of deletion mutant with cAMP binding domains. Science 1995;269:807-813.
13. Diller TC, Madhusudan, Xuong, N-h, Taylor, S.S. Molecular basis for regulatory subunit diversity in cAMP-dependent protein kinase: crystal structure of the type IIb regulatory subunit. Structure 2001;9:73-82.
14. Newlon MR, M, Morikis, D, Carr, DW, Westphal, R, Scott, JD, Jennings, PA. A novel mechanism of PKA anchoring revealed by solution structures of anchoring complexes. EMBO J 2001;20:1651-1662.
15. Tortora G, Damiano V, Bianco C, Baldassarre G, Bianco AR, Lanfrancone L, Pelicci PG, Ciardiello F. The RIalpha subunit of protein kinase A (PKA) binds to Grb2 and allows PKA interaction with the activated EGF-receptor. Oncogene 1997;14:923-928.
16. Rosen OM, Erlichman J. Reversible autophosphorylation of a cyclic 3′:5′-AMP-dependent protein kinase from bovine cardiac muscle. J Biol Chem 1975;250:7788-7794.
17. Carmichael DF, Geahlen RL, Allen SM, Krebs EG. Type II regulatory subunit of cAMP-dependent protein kinase. Phosphorylation by casein kinase II at a site that is also phosphorylated in vivo. J Biol Chem 1982;257:10440-10445.
18. Hemmings BA, Aitken A, Cohen P, Rymond M, Hofmann F. Phosphorylation of the type-II regulatory subunit of cyclic-AMP-dependent protein kinase by glycogen synthase kinase 3 and glycogen synthase kinase 5. Eur J Biochem 1982;127:473-481.
19. Keryer G, Luo Z, Cavadore JC, Erlichman J, Bornens M. Phosphorylation of the regulatory subunit of type II beta camp-dependent protein kinase by cyclin b/p34cdc2 kinase impairs its binding to microtubule-associated protein 2. Proc Natl Acad Sci U S A 1993;90:5418-5422.
20. Keryer G, Yassenko M, Labbe JC, Castro A, Lohmann SM, Evain-Brion D, Tasken K. Mitosis-specific phosphorylation and subcellular redistribution of the RIIalpha regulatory subunit of cAMP-dependent protein kinase. J Biol Chem 1998;273:34594-34602.
21. Gangal M, Li F, Jones JM, Deich J, Lovett K, Taylor SS, Johnson DA. Consequences of cAMP and catalytic subunit binding on the flexibility of the A-kinase regulatory subunit. Biochemistry 2000; 39:15626-15632.
22. Diller TC, Xuong N-h, Taylor SS. Type IIβ regulatory subunit of cAMP-dependent protein kinase: Purification strategies to optimize crystallization. Protein Expr Purif 2000;20:357-364.
23. Builder SE, Beavo JA, Krebs EG. Stoichiometry of cAMP and 1,N6-etheno-cAMP binding to protein kinase. J Biol Chem 1980; 225:2350-2354.
24. Lakowicz JR. Principles of fluorescence spectroscopy. New York: Plenum; 1983. 496 p.
25. Pace CN. Determination and analysis of urea and guanidine hydrochloride denaturation curves. Methods Enzymol 1986;131:266-280.
26. Chen RF. Fluorescence quantum yields of tryptophan and tyrosine. Anal Lett 1967;1:35-42.
27. Beechem J. A second generation global analysis program for the recovery of complex inhomogeneous fluorescence decay kinetics. Chem Phys Lipids 1989;50:237-251.
28. Chen Y, Liu B, Yu HT, Barkley, MD. The peptide bond quenches indole fluorescence. J Am Chem Soc 1996;118:9271-9278.
29. Chen RF, Knutson JR, Ziffer H, Porter D. Fluorescence of tryptophan dipeptides: correlations with the rotamer model. Biochemistry 1991;30:5184-5195.
30. Carrasco B, de la Torre, JG. Improved hydrodynamic interaction in macromolecular bead models. J Chem Phys 1999;111:4817-4826.
31. Zhao J, Hoye E, Boylan S, Walsh DA, Trewhella J. Quaternary structures of a catalytic subunit-regulatory subunit dimeric complex and the holoenzyme of the cAMP-dependent protein kinase by neutron contrast variation. J Biol Chem 1998;273:30448-30459.
32. Shen X, Knutson JR. Subpicosecond fluorescence spectra of tryptophan in water. J Phys Chem B 2001;105:6260-6265.
33. Eftink MR. The use of fluorescence methods to monitor unfolding transitions in proteins. Biophys J 1994;66:482-501.
34. Leon DA, Canaves JM, Taylor SS. Probing the multidomain structure of the type I regulatory subunit of cAMP-dependent protein kinase using mutational analysis: Role and environment of endogenous tryptophans. Biochemistry 2000;39:5662-5671.
35. LaPorte DC, Builder SE, Storm DR. Spectroscopic studies of the cAMP binding sites of the regulatory subunits of type I and II protein kinase. J Biol Chem 1980;255:2343-2349.
36. Potter RL, Taylor SS. Relationships between structural domains and function in the regulatory subunit of cAMP-dependent protein kinases I and II from porcine skeletal muscle. J Biol Chem 1979;254:2413-2418.
37. Potter RL, Taylor SS. Correlation of the cAMP binding domain with a site of autophosphorylation on the regulatory subunit of cAMP-dependent protein kinase II from porcine skeletal muscle. J Biol Chem 1979;254:9000-9005.
38. Dell'Acqua ML, Scott, JD. Protein kinase A anchoring. J Biol Chem 1997;272:12881-12884.
39. Herberg FW, Zimmermann B, McGlone M, Taylor SS. Importance of the A-helix of the catalytic subunit of cAMP-dependent protein kinase for stability and for orienting subdomains at the cleft interface. Prot Sci 1997;6:569-579.
40. Gangal M, Clifford T, Deich J, Cheng X, Taylor SS, Johnson DA. Mobilization of the A-kinase N-myristate through an isoformspecific intermolecular switch. Proc Natl Acad Sci USA 1999;96:12394-12399.
41. Cheng X, Phelps C, Taylor SS. Differential binding of cAMP-dependent protein kinase regulatory subunit isoforms Ialpha and IIbeta to the catalytic subunit. J Biol Chem 2001;276:4102-4108.