Expression of immunologically active porcine recombinant TGF-β1 precursor protein in baculovirus system

Journal of Microbiology

Volumn 35, Issue 4, 1997, Pages 341-346

  Lim, Hyun ^a   Kim, Pyeung Hyenn ^a   Chun, Gie Taek ^a   Choi, Eui Yul ^b   Yie, Se Won ^a  

^a Kangwon National University   (South Korea)

^b Hallym University   (South Korea)

Author keywords

Baculovirus expression; Precursor; Recombinant protein; TGF 1

Indexed keywords

EID: 0008713388     PISSN: 12258873     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Assoian, R.K., A. Komoriya, C.A. Meyers, D.M. Smith, and M.B. Sporn. 1983. Transforming growth factor beta in human platelets: Identification of a major storage site, purification and characterization. J. Biol. Chem. 258, 7155-7160.
2. Barber, G.N., J.C.S. Clegg, and G. Lloyd. 1990. Expression of the Lassa virus nucleocapsid protein in insect cells infected with a recombinant baculovirus: application to diagnostic assays for Lassa virus infection. J. Gen. Virol. 71, 19-28.
3. Bourdrel, L., C.-H. Lin, S.L. Lauren, H.H. Elmore, B. J. Sugarman, S. Hu, and K.R. Westcott. 1993. Recombinant human transforming growth factor-β1: Expression by Chinese hamster ovary cells, isolation, and characterization. Protein Expression and Purification 4, 130-140.
4. Brunner, A.M., H. Marquatrdt, A.R. Malacko, M.N. Lioubin, and A.F. Purchio. 1989. Site-directed mutagenesis of cysteine residues in the pro region of the trasforming growth factor beta1 precursor. Expression and characterization of mutant proteins. J. Biol. Chem. 264, 13660-4.
5. Choi, E.Y., P.H. Kim, and W-H. Byeon. 1997. Expression of immunologically detectable epitope having porcine transforming growth factor-β1 (TGF-β1) in Escherichia coli. Kor. J. Appl. Microbiol. Biotechnol. 25, 137-143.
6. Derynck, R., J.A. Jarrett, E.Y. Che, and D.V. Goeddel. 1986. The murine transforming growth factor-β precursor. J. Biol. Chem. 261, 4377-4379.
7. Kondaiah, P., E.V. Obberghen-Schilling, R.L. Ludwig, R. Dhar, M.B. Sporn, and A.B. Roberts. 1988. cDNA cloning of porcine transforming growth factor-1 mRNAs. J. Biol. Chem. 263, 18313-18317.
8. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680-685.
9. Luckow, V.A., S.C. Lee, G.F. Barry, and P.O. Olins. 1993. Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli. J. Virol. 67, 4566-4579.
10. McCartney-Francis, N.L. and S.M. Wahl. 1994. Transforming growth factor beta: a matter of life and death. J. Leukocyte Biology 55, 401-409.
11. Miyazono, K., A. Olofsson, P. Colosetti, and C.-H. Heldin. 1991. A role of the latent TGF-β1-binding protein in the assembly and secretion of TGF-β1. The EMBO J. 10, 1091-1101.
12. Rhee, K.J., S.-U. Nham, J.-S. Yoo, S.W. Yie, G.-T. Chun, E.Y. Choi, J.-H. Park, and P.-H. Kim. 1996. Characterization of mutated transforming growth factor-β1 transformants and its modulatory effect on IgA isotype synthesis by murime B lymphocytes. Mol. Cells 6, 746-752.
13. Seyedin, S.M., T.C. Thomas, A.Y. Thompson, D.M. Rosen, and K.A. Picz. 1985. Purification and characterization of two cartilage-inducing factors from bovine demineralized bone. Proc. Natl. Acad. Sci. USA 82, 267-2271.
14. Tuan, T.L., D.T. Cheung, L.T. Wu. A. Yee, S. Gabriel, B. Han, L. Morton, M.E. Nimni, and F.L. Hall. 1996. Engineering, expression and renaturation of targeted TGF-beta fusion proteins. Connet. Tissue. Res. 34, 1-9.