Exprssion of Immunologically Detectable Epitope Having Porcine Transforming Growth Factor-β1 (TGF-β1) in Escherichia coli

Korean Journal of Applied Microbiology and Biotechnology

Volumn 25, Issue 2, 1997, Pages 137-143

  Choi, Eun Young ^a   Kim, Pyung Hyun ^a   Byeon, Woo Hyeon ^a  

^a Kangwon National University   (South Korea)

Author keywords

E. coli system; pGEX 1 T; Porcine TGF 1; Recombinant plasmid

Indexed keywords

EID: 0008685479     PISSN: 02572389     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Bowden, G. A., A. M. Parades, and G. Georgiou. 1991. Structure and morphology of protein inclusion bodies in E. coli. Bio/Technology 9: 725-726.
2. Derynck, R. 1987. Structure of transforming growth factors-α and -β and their precursors, Pp. 133-163. In Garoff G.(ed.), Oncogenes, genes and growth factors, A Wiley-Interscience Publication.
3. Derynck, R., J A. Japrett, E. Y. Chen, D. H. Eaton, J. R. Bell, R. K. Assoian, A. B. Roberts, M. B, Sporn, and D. V. Goeddel. 1985. Human transforming growth factor-β1 complementary DNA sequence and expression of normal and transformed cell. Nature 316:701-705.
4. Derynck, R., L. Rhee, E. Y. Chen, and A. V. Tilburg. 1987. Intron-exon structure of the human transforming growth factor-β precursor gene. Nucleic Acids Res. 15: 3188-3189.
5. Derynck, S. and L. Rhee. 1987. Sequence of the porcine transforming growth factor-beta precursor. Nucleic. Acids Res. 15: 3187.
6. Eaton, D., H. Rodriguez, and G. A. Vehar. 1986. Proteolytic processing of human factor VIII. Correlation of specific cleavages by thrombin, factor Xa, and activated protein C with activation and inactivation of factor VIII coagulant activity. Biochemistry. 25: 505-512.
7. Frangioni, J. V. and B. G. Neel. 1993. Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal. Biochem. 210: 179-187.
8. Frankel, S., R. Sohn, and L. Leinwand. 1991. The use of sarcosyl in generating soluble protein after bacterial expression. Proc. Natl. Acad. Sci. 88: 1192-1196.
9. Kagnoff, M. F. and P-H. Kim. 1991. Effects of Transforming growth factor-β1 and interlukin-5 on IgA isotype switching at the clonal level. Immunologic Research 10: 396-399.
10. Kim, P-H. and M. F. Kagnoff. 1990. Transforming growth factor-β1 stimulates IgA isotype switching. J. Immunol. 145: 3773-3778.
11. Kondaiah, P., E. B. Obberghen-Schilling, R. L. Ludwig, R. Dhar, M. B. Sporn, and A. B. Roberts. 1988. cDNA cloning of porcine transforming growth factor-β1 mRNAs. J. Biol. Chem. 263: 18313-18317.
12. Lyons, R. M., J. Keski-Oja, and H. E. Moses. 1988. Proteolytic activation of latent transforming growth factor-β1 from fibroblast-conditioned medium. J. Cell Biol. 106: 1659-1665.
13. Marston, F. A. O. 1986. The purification of eucaryotic polypeptides synthesized in E. coli. Biochem. J. 240: 1-12.
14. Marston, F. A. O. and D. L. Hartley. 1990. Solubilization of protein aggregates. Methods in Enzymology 182: 265-276.
15. Miyazono, K., C. H. Heldin. 1989. Role of carbohydrate structures in TGF-β1 latency. Nature 338: 158-160.
16. Okayama, H. and P. Berg. 1983. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Mol. Cell Biol. 3: 280-289.
17. Roberts, A. B., S. J. Kim, P. Kondaiah, S. B. Jakowlew, F. Denhez, A. B. Glick, A. G. Geiser, S. Watanabe, T. Noma, R. Lechleider, and M. B. Sporn. 1990. Transcriptional control of expression of the TGF-βs. Ann. New York Acad. Sci. 593: 43-58.
18. Seo, K-B., S. E. Yie, I. Choi, K-H. Pyun, P-H. Kim, 1992. Regulation of transforming growth factor-β1 synthesis by murine T lymphocytes and its effect on IgA antibody response. Korean J. Immunology 14: 203-211.
19. Smith, D. B., L. C. Berger, and A. G. Wildman. 1993. Modified glutathions S-transferase fusion proteins for simplified analysis of protein-protein interactions. Nucleic Acids Res. 21: 359-360.
20. Smith, D. B., and K. S. Johnson. 1988. Single-step purification of polypeptides expressed in E. coli as fusion with glutathione S-transferase. Gene 67: 31-40.
21. Tabor, S. and C. C. Richardson: 1985. A bacteriophage T7 polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. 82: 1074-1078.