Evaluation of a new assay for HBV DNA quantitation in patients with chronic hepatitis B

Clinical and Diagnostic Virology

Volumn 9, Issue 1, 1998, Pages 37-43

  Kessler, Harald H ^a   Pierer, Karen ^a   Dragon, Elizabeth ^b   Lackner, Herwig ^a   Santner, Brigitte ^a   Stünzner, Doris ^a   Stelzl, Evelyn ^a   Waitzl, Brigitte ^a   Marth, Egon ^a  

^a UNIVERSITY OF GRAZ   (Austria)

^b HOFFMANN LA ROCHE INC   (United States)

Author keywords

Amplicor(TM) HBV monitor test; Digene Hybrid Capture(TM) system HBV DNA assay; HBV therapy; Hepatitis B virus; Polymerase chain reaction; Routine diagnostic laboratory

Indexed keywords

ALPHA INTERFERON; PREDNISOLONE; VIRUS DNA;

ACCURACY; ARTICLE; ASSAY; CHILD; CHRONIC HEPATITIS; CONTROLLED STUDY; DNA DETERMINATION; HEPATITIS B; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MONITOR; PRIORITY JOURNAL; REPRODUCIBILITY;

CHILD; DNA, VIRAL; HEPATITIS B VIRUS; HEPATITIS B, CHRONIC; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0007466734     PISSN: 09280197     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-0197(97)10008-3     Document Type: Article

References (27)

1. Barlet V., Cohard M., Thelu M.A., Chaix M.J., Baccard C., Zarski J.P., Seigneurin J.M. Quantitative detection of hepatitis B virus DNA in serum using chemiluminescence:comparison with radioactive solution hybridization assay. J. Virol. Methods. 49:1996;141-151.
2. Carman W.F., Dourakis S., Karayiannis P., Crossey M., Drobner R., Thomas H.C. Incidence of hepatitis B viremia, detected using the polymerase chain reaction, after successful therapy of hepatitis B virus carriers with interferon-a. J. Med. Virol. 34:1991;114-118.
3. De Lamballerie X., Gallian P., De Micco P.H. Evaluation of a chemiluminescent molecular hybridization assay for the detection and quantitation of hepatitis B virus-DNA in serum. New Perspect. Clin. Microbiol. 18:1995;207-213.
4. Erhardt A., Schaefer S., Athanassiou N., Kann M., Gerlich W.H. Quantitative assay of PCR-amplified hepatitis B virus DNA using a peroxidase-labelled DNA probe and enhanced chemiluminescence. J. Clin. Microbiol. 34:1996;1885-1891.
5. the Eurohep Group, Gerlich W.H., Heermann K.H., Thomssen R. Quantitative assays for hepatitis B virus DNA: standardization and quality control. Viral Hep. Rev. 1:1995;53-57.
6. Heermann K.H., Hagos Y., Thomssen R. Liquid-phase hybridization and capture of hepatitis B virus DNA with magnetic beads and fluorescence detection of PCR products. J. Virol. Methods. 50:1995;43-58.
7. Hendricks D.A., Stowe B.J., Hoo B.S., Kolberg J., Irvine B.D., Neuwald P.D., Urdea M.S., Perrillo R.P. Quantitation of HBV DNA in human serum using a branched DNA (bDNA) signal amplification assay. Am. J. Clin. Pathol. 104:1995;537-546.
8. Hepatitis Interventional Therapy Group, Perrillo R.P., Schiff E.R., Davis G.L., Bodenheimer H.C., Lindsay K., Payne J., Dienstag J.L., O'Brien C., Tamburro C., Jacobson I.M., Sampliner R., Feit D., Lefkowitch J., Kuhns M., Meschievitz C., Sanghvi B., Albrecht J., Gibas A. A randomized, controlled trial of interferon alfa-2 b alone and after prednisone withdrawal for the treatment of chronic hepatitis B. New Engl. J. Med. 323:1990;295-301.
9. Izopet J., Poggi C., Dussaix E., Mansuy J.M., Cubaynes L., Profizi N., Lafeuillade A., Marchou B., Massip P., Sayada C., Puel J. Assessment of a standardized reverse-transcriptase PCR assay for quantifying HIV-1 RNA in plasma and serum. J. Virol. Methods. 60:1996;119-129.
10. Jalava T., Ranki M., Bengtström M., Pohjanpelto P., Kallio A. A rapid and quantitative solution hybridization method for detection of HBV DNA in serum. J. Virol. Methods. 36:1992;171-180.
11. Jardi R., Buti M., Rodriguez-Frias F., Cortina M., Esteban R., Guardia J., Pascual C. The value of quantitative detection of HBV-DNA amplified by PCR in the study of hepatitis B infection. J. Hepatol. 24:1996;680-685.
12. Kaneko S., Miller R.H., Feinstone S.M., Unoura M., Kobayashi K., Hattori N., Purcell R.H. Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proc. Natl. Acad. Sci. USA. 86:1989;312-316.
13. Kapke G.F., Watson S., Sheffler S., Hunt D., Frederick C. Comparison of the Chiron Quantiplex branched DNA (bDNA) assay and the Abbott Genostics solution hybridization assay for quantification of hepatitis B viral DNA. J. Viral Hep. 4:1997;67-75.
14. Kessler H.H., Santner B.I., Umlauft F., Kronawetter M., Stünzner D., Pierer K., Stelzl E., Grünewald K., Marth E. Quantitation and genotyping of hepatitis C virus RNA in sera of hemodialysis and AIDS patients. Clin. Diagn. Virol. 5:1996;73-78.
15. Kuhns M.C., McNamara A.L., Perrillo R.P., Cabal C.M., Campbel C.R. Quantitation of hepatitis B viral DNA by solution hybridization: comparison with DNA polymerase and hepatitis B e antigen during antiviral therapy. J. Med. Virol. 27:1989;274-281.
16. Lazizi Y., Pillot J. Delayed clearance of HBV-DNA detected by PCR in the absence of viral replication. J. Med. Virol. 39:1993;208-213.
17. Lehtovaara P., Uusi-Oukari M., Buchert P., Laaksonen M., Bengtström M., Ranki M. Quantitative PCR for hepatitis B virus with colorimetric detection. PCR Methods Appl. 3:1993;169-175.
18. Lieberman H.M., LaBrecque D.R., Kew M.C., Hadziyannis S.J., Shafritz D.A. Detection of hepatitis B virus DNA directly in human serum by a simplified molecular hybridization test: Comparison to HBeAg/anti-HBe status in HBsAg carriers. Hepatology. 3:1983;285-291.
19. Lok A.S.F. Treatment of chronic hepatitis B. J. Viral Hep. 1:1994;105-124.
20. Miskovsky E.P., Carrella A.V., Gutekunst K., Sun C.A., Quinn T.C., Thomas D.L. Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus. J. Clin. Microbiol. 34:1996;1975-1979.
21. Niitsuma H., Ishii M., Miura M., Kobayashi K., Toyota T. Low level hepatitis B viremia detected by polymerase chain reaction accompanies the absence of HBe antigenemia and hepatitis in hepatitis B virus carriers. Am. J. Gastroenterol. 92:1997;119-123.
22. Pastore G., Santantonio T., Milella M., Monno L., Mariano N., Moschetta R., Pollice L. Anti-HBe-positive chronic hepatitis B with HBV-DNA in the serum response to a 6-month course of lymphoblastoid interferon. J. Hepatol. 14:1992;221-225.
23. Ranki M., Schätzl H.M., Zachoval R., Uusi-Oukari M., Lehtovaara P. Quantification of hepatitis B virus DNA over a wide range from serum for studying viral replicative activity in response to treatment and in recurrent infection. Hepatology. 21:1995;1492-1499.
24. Scotto J., Hadchouel M., Hery C., Yvart J., Tiollais P., Brechot C. Detection of hepatitis B virus DNA in serum by a simple spot hybridization technique: comparison with results for other viral markers. Hepatology. 3:1983;279-284.
25. Urdea M.S., Running J.A., Horn T., Clyne J., Ku L., Warner B.D. A novel method for the rapid detection of specific nucleotide sequences in crude biological samples without blotting or radioactivity: application to the analysis of hepatitis B virus in human serum. Gene. 61:1987;253-264.
26. Vandamme A.M., Schmit J.C., Van Dooren S., Van Laethem K., Gobbers E., Kok W., Goubau P., Witvrouw M., Peetermans W., De Clercq E., Desmyter J. Quantification of HIV-1 RNA in plasma: comparable results with the NASBA HIV-1 RNA QT and the AMPLICOR HIV monitor test. J. Acquired Immune Defic. Syndr. 13:1996;127-139.
27. Zaaijer H.L., Ter Borg F., Cuypers H.T.M., Hermus M.C.A.H., Lelie P.N. Comparison of methods for detection of hepatitis B virus DNA. J. Clin. Microbiol. 32:1994;2088-2091.