Amplification de séquences (RT-PCR) et analyse du polymorphisme des fragments de restriction (RFLP) de quelques isolats du Prunus necrotic ringspot ilarvirus

EPPO Bulletin

Volumn 31, Issue 2, 2001, Pages 173-178

  Boulila, M ^a   Marrakchi, M ^b  

^a INSTITUT DE L OLIVIER   (Tunisia)

^b FACULTÉ DES SCIENCES DE TUNIS   (Tunisia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0005670755     PISSN: 02508052     EISSN: 13652338     Source Type: Journal    
DOI: 10.1111/j.1365-2338.2001.tb00987.x     Document Type: Article

References (18)

1. Boulila M (1992a) Mise en évidence de trois ilarvirus associés à la mosaïque de l’amandier dans une pépinière fruitière au Cap Bon (Tunisie). Annales de L’inrat 65, 49–59.
2. Boulila M (1992b) Détection du virus des taches annulaires nécrotiques des Prunus (PNRSV) dans un verger de prunier au Cap Bon (Tunisie). Annales de L’inrat 65, 61–73.
3. Boulila M (1997) Occurrence and detection of three ilarviruses infecting almond and plum crops in Tunisia. In Proceedings of the 10th Congress of the Mediterranean Phytopathological Union, pp. 129–134. Société Française de Phytopathologie, ORSTOM, Montpellier (FR).
4. Cambra M, Olmos A, Asensio M, Esteban O & Gorris MT (1998) Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, Heminested-PCR and PCR-ELISA. Acta Horticulturae no. 472, 257–263.
5. Candresse T, Kofalvi S, Lanneau M & Dunez J (1998) Detection of fruit tree viruses and viroids using PCR-based assays. In Advances in the Detection of Plant Pathogens by Polymerase Chain Reaction (eds Manceau C & Spak P). COST 823, EU Commission, Luxembourg.
6. Crescenzi A, D’Aquino L, Comes S, Nuzzaci M, Piazzolla P & Hadidi A (1997) Characterization of the sweet cherry isolate of plum pox potyvirus. Plant Disease 81, 711–714.
7. DNASIS (1991, 1993). MacDNASIS (Macintosh, DNA and protein sequence analysis system). HITACHI Software Engineering Co.
8. Hadidi A, Levy L & Podleckis EV (1995) Polymerase chain reaction technology in plant pathology. In Molecular Methods in Plant Pathology (eds Singh RP & Singh US), pp. 167–187. CRC Press, Boca Raton (US).
9. Hammond RW & Crosslin JM (1995) The complete nucleotide sequence of RNA 3 of a peach isolate of prunus necrotic ringspot virus. Virology 208, 349–353.
10. Hammond RW, Crosslin JM, Howell WE & Mink GI (1998) Strain-specific polymerase chain reaction assays for discrimination of prunus necrotic ringspot virus isolates. Acta Horticulturae no. 472, 235–242.
11. Kölber M, Németh M, Krisbai L, Szemes M, Kiss-Tóth E, Dorgai L & Kálmán M (1998) Detectability of prunus necrotic ringspot and plum pox viruses by RT-PCR, Multiplex RT-PCR, ELISA and indexing on woody indicators. Acta Horticulturae no. 472, 243–247.
12. Nemchinov L, Hadidi A & Faggioli F (1998) PCR-detection of apple stem pitting virus from pome fruit hosts and sequence variability among viral isolates. Acta Horticulturae no. 472, 67–73.
13. Németh M (1986) Virus, Mycoplasma and Rickettsia Diseases of Fruit Trees. Akademiai Kiado, Budapest (HU).
14. Rosner A, Shiboleth Y, Spiegel S, Krisbai L & Kölber M (1998) Evaluating the use of immunocapture and sap-dilution PCR for the detection of prunus necrotic ringspot virus. Acta Horticulturae no. 472, 227–233.
15. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning. A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York (US).
16. Spiegel S, Scott SW, Bowman-Vance V, Tarn Y, Galiakparov N & Rosner A (1996) Improved detection of prunus necrotic ringspot virus by the polymerase chain reaction. European Journal of Plant Pathology 102, 681–685.
17. Spiegel S, Tam Y, Maslenin L, Kölber M, Németh M & Rosner A (1999) Typing prunus necrotic ringspot virus isolates by serology and restriction endonuclease analysis of PCR products. Annals Applied Biology 135, 395–400.
18. Zeramdini H, Di Terlizzi B & Savino V (1996) Phytosanitary status of almond and apricot in Tunisia. Bulletin OEPP/EPPO Bulletin 26, 155–160.