Detection and typing of Prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA

Acta Horticulturae

Volumn 472, Issue , 1998, Pages 257-263

  Cambra, M ^a   Olmos, A ^a   Asensio, M ^a   Esteban, O ^a   Gorris, M T ^a   Boscia, D ^b   Candresse, T ^c  

^a INSTITUTO VALENCIANO DE INVESTIGACIONES AGRARIAS IVIA   (Spain)

^b UNIVERSITY OF BARI   (Italy)

^c CEMAGREF   (France)

Author keywords

Aphids; ELISA; Monoclonal antibodies; PCR; Prunus viruses; Sharka

Indexed keywords

APHIDIDAE; APHIS GOSSYPII; APPLE CHLOROTIC LEAF SPOT VIRUS; APPLE MOSAIC VIRUS; HEXAPODA; ILARVIRUS; MALUS X DOMESTICA; PLUM POX VIRUS; POTYVIRUS; PRUNUS; PRUNUS ARMENIACA; PRUNUS DOMESTICA; TRICHOVIRUS;

EID: 0042100455     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/actahortic.1998.472.28     Document Type: Conference Paper

References (14)

1. Boscia D., Zeramdini H., Cambra M., Potere O., Gorris M. T., Myrta A., Di Terlizzi B. and Savino V., 1997. Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. Eur. J. Plant Pathol. 103:477-480.
2. Cambra M., Asensio M., Gorris M. T., Pérez E., Camarasa E., García J. A., Moya J. J., López-Abella D., Vela C., and Sanz A., 1994. Detection of plum pox potyvirus using monoclonal antibodies to structural and non-structural proteins. Bulletin OEPP/EPPO Bulletin 24:569-577.
3. Cambra M., Camarasa E., Gorris M. T., Gamsey S. M. and Carbonell E., 1991. Comparison of different immunosorbent assays for citrus tristeza virus (CTV) using CTV-specific monoclonal and polyclonal antibodies.. In: Proceedings of the 11th International Organization of Citrus Virologists, IOCV, Riverside. Brlansky, R. H., Lee, R. F., Timmer, L. W., eds. Pp. 38-45.
4. Cambra M., Olmos A., Candresse T., Gorris M. T., 1997a. Detection of Prunus viruses by print-capture PCR. Bulletin OEPP/EPPO Bulletin (in press).
5. Cambra M., Olmos A., Gorris M. T., Duran N., Román M. P., Camarasa E., Dasí M. A., 1997b. Sensitive detection of plant pathogens by using immobilized targets in tissue imprinted membranes. In: Diagnosis and Identification of Plant Pathogens. H. W. Dehne et al., eds. Kluwer Academic Publishers, Dordrecht. Pp. 95-98.
6. Candresse T., Cambra M., Dallot S., Lanneau M., Asensio M., Gorris M. T., Revers E, Macquaire G., Olmos A., Boscia D., Quiot J. B., and Dunez J., 1998. Comparison of monoclonal antibodies and PCR assays for the typing of isolates belonging to the D and M serotypes of plum pox potyvirus. Phytopathology 88:198-204.
7. Candresse T., Kofalvi S. A., Lanneau M., Dunez J., 1998. A PCR-ELISA procedure for the simultaneous detection and identification of prunus necrotic ringspot (PNRSV) and apple mosaic (ApMV) ilarviruses. Acta Horticulturae (this volume).
8. Candresse T., Lanneau M., Revers F., Grasseau N., Macquaire G., German S., Malinovsky T., and Dunez J., 1995a. An immunocapture PCR assay adapted to the detection and the analysis of the molecular variability of the apple chlorotic leafspot virus. Acta Horticulturae 386:136-147.
9. Candresse T., Macquaire G., Lanneau M., Bousalem M., Quiot-Douine L., Quiot J. B., Dunez J. 1995b. Analysis of plum pox potyvirus variability and development of strain-specific PCR assays. Acta Horticulturae 386:357-369.
10. Clark M. F. and Adams A. N., 1977. Characteristics of the microplate method of enzymelinked immunosorbent assay for the detection of plant viruses. J. Gen. Virology 34:475-483.
11. Olmos A., Cambra M., Dasí M. A., Candresse T., Esteban O., Gorris M. T., and Asensio, M., 1997. Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by Heminested-PCR and PCR-ELISA. J. Virol. Methods 68:127-137.
12. Olmos A., Dasí M. A., Candresse T., Cambra M., 1996. Print-capture PCR: a simple and higly sensitive method for the detection of plum pox virus (PPV) in plant tissues. Nucleic Acids Res. 24:2192-2193.
13. Wetzel T., Candresse T., Macquaire G., Ravelonandro M., and Dunez J., 1992. A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. J. Virol. Methods 39:27-37.
14. Wetzel T., Candresse T., Ravelonandro M., and Dunez J., 1991. A polymerase chain reaction assay adapted to plum pox potyvirus detection. J. Virol. Methods 33:355-365.