The use of acid Polyacrylamide gel electrophoresis to determine the effect of locality of growth on the protein profiles of barley, wheat and honeybush tea

South African Journal of Plant and Soil

Volumn 14, Issue 3, 1997, Pages 131-136

  de Villiers, O T ^a   Bosman, M ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Acid Polyacrylamide gel electrophoresis (A PAGE); Barley; Extraction; Heuningbostee; Location; Seed identification; Spring wheat

Indexed keywords

CYCLOPIA; HORDEUM VULGARE SUBSP. VULGARE; TRITICUM AESTIVUM;

EID: 0005378214     PISSN: 02571862     EISSN: None     Source Type: Journal    
DOI: 10.1080/02571862.1997.10635095     Document Type: Article

References (22)

1. Bushuk, W., and Zillman, P. R., 1978. Wheat cultivar identification by gliadin electrophoregrams 1. Apparatus, method and nomenclature. Can. J. Plant Sci., 58:505–515.
2. Clements, R. L., 1987. A study of gliadins of soft wheats from the eastern United States using a modified Polyacrylamide gel electrophoresis procedure. Cereal Chem., 64:442–448.
3. Clydesdale, Al, Draper, S. R., and Craig, E. A., 1982. A standard method for the identification of wheat varieties by electrophoresis of their gliadins. J. Natn. Inst. Afric. Bot., 16:61–66.
4. Cooke, R. J, and Cliff, E. M., 1983. Barley cultivar characterization by electrophoresis. I. A method for acid Polyacrylamide gel electrophoresis of hordein proteins. J. Natn. Inst. Afric. Bot., 16:189–195.
5. Cooke, R. J, Cliff, E. M., and Draper, S. R., 1983. Barley cultivar characterization by electrophoresis. 11. Classification of hordein electrophoregrams. J. Natn. Inst. Agric. Bot., 16:197–206.
6. De Villiers, O. T., and Bosman, M., 1993. Wheat cultivar identification by electrophoretic analysis of gliadin proteins. S. Afr. Plant Soil, 10 (3):99–104. 1993
7. De Villiers, O. T., and Bosman, M., 1996. Analysis of the HMW glutenin subunit composition of wheat cultivars by SDS-PAGE. S. Afr. J. Plant Soil, 13 (1):1–4.
8. De Villiers, O. T., and Laubscher, E. W., 1989. Barley cultivar identification by acid Polyacrylamide gel electrophoresis of hordein proteins. S. Afr. J. Plant Soil, 6:70–74.
9. Du Cros, D. L., and Wrigley, C. W., 1979. Improved electrophoretic methods for identifying cereal varieties. J. Sci. Fd. Agric., 30:785–794.
10. Gebre, H., Khan, K., and Foster, A. E., 1986. Barley cultivar identification by Polyacrylamide gel electrophoresis of hordein proteins:Catalogue of cultivars. Crop Sci., 26:254–260.
11. Jones, B. L, Lookhart, G. L, Hall, S. B., and Finney, K. F., 1982. Identification of wheat cultivars by gliadin electrophoresis:Electrophoregrams of 88 wheat cultivars most commonly grown in the United States in 1979. Cereal Chem., 59:181–188.
12. Khan, K., Hamada, A. S., and Patek, J., 1985. Polyaciylamide gel electrophoresis for wheat variety identification:effects of variables on gel properties. Cereal Chem., 62:310–313.
13. Khan, K., Mcdonald, C. E., and Banasik, O. J., 1983. Polyacrylamide gel electrophoresis of gliadin proteins for wheat variety identification—procedural modifications and observations. Cereal Chem., 60:178–181.
14. Lookhart, G. L, Jones, B. L, Hall, S. B., and Finney, K. F., 1982. An improved method for standardizing Polyacrylamide gel electrophoresis of wheat gliadin proteins. Cereal Chem., 59:178–181.
15. Marais, J. S.C., and Smuts, D. B., 1940. 205–210. The biological values of the protein of oats, barley, wheatbran and pollard. Onderstepoort Journal of Veterinary Science and Animal Industry, 15 (1+2)
16. Marchylo, B. A., and La Berge, D. E., 1980. Barley cultivar identification by electrophoretic analysis of hordein proteins. 1. Extraction and separation of hordein proteins and environmental effects on the hordein electrophoregram. Can. J. Plant Sci., 60:1343–1350.
17. Marchylo, B. A., and La Berge, D. E., 1981. Barley cultivar identification by electrophoretic analysis of hordein proteins. II. Catalogue of electrophoregram formulae for Canadian-grown barley cultivars. Can. J. Plant Sci., 61:809–830.
18. Montembault, A., Autran, J. C., and Joudrier, P., 1983. Varietal identification of barley and malt. J. Inst. Brew., 89:299–302.
19. Salmon, S., and Burgridge, K. M., 1985. Wheat variety identification by Polyacrylamide gel electrophoresis. FMBRA Bulletin, 2:78–88.
20. Shewry, P. R., Ellis, J. R.S., Pratt, H. M., and Miflin, B. J., 1978. A comparison of methods for the extraction and separation of hordein fractions from 29 barley varieties. J. Sci. Fd. Agric., 29:433–441.
21. Smith, D. B., and Payne, P. I., 1984. A procedure for the routine determination of electrophoretic band patterns of barley and malt endosperm proteins. J. Natn. Inst. Agric. Bot., 16:487–498.
22. Zillman, R. R., and Bushuk, W., 1979. Wheat cultivar identification by gliadin electrophoregrams. III. Catalogue of electrophoregram formulas of Canadian wheat cultivars. Can. J. Plant Sci., 59:287–298.