Advantages of the methylotrophic yeast Pichia pastoris for high-level expression and purification of heterologous proteins

Australasian Biotechnology

Volumn 6, Issue 2, 1996, Pages 82-87

  Sberna, Gian ^a   Cappai, Roberto ^a   Henry, Anna ^a   Small, David H ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0003799446     PISSN: 10367128     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (42)

1. Barr, K.A., Hopkins, S.A. and Sreekrishna, K. (1992) Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharm. Eng. 12, 48-51.
2. Buckholz, R.G. and Gleeson, M.A. (1991) Yeast systems for the commercial production of heterologous proteins. Biol technology 9, 1067-1072.
3. Clare, J.J., Rayment, F.B., Ballantine, S.P., Sreekrishna, K. and Romanas, M.A. (1991a) High-level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integration to the gene. Bio/technology 9, 455-460.
4. Clare, J.J., Romanas, M.A., Rayment, F.B., Rowedder, J.E., Smith, M.A., Payne, M.M., Sreekrishna, K. and Henwood, C. A. (1991b) Production of mouse epidermal growth factor in yeast: high level secretion using Pichia pastoris strains containing multiple gene copies. Gene 105, 205-212.
5. Couderc, R. and Baratti, J. (1980) Oxidation of methanol by the yeast Picliia pastoris: purification and properties of alcohol oxidase. Agric. Biol. Chem. 44, 2279-2289.
6. Cregg, J.M., Tscopp, J., Stillman, C., Siegel, R., Akong, M., Craig, W.S., Buckholz, R.G., Madden, K.R., Kellaris, P.A., Davis, G.R., Smiley, B.L., Cruze, J., Torregrossa, R., Velicelebi, G. and Thill, G.P. (1987) High-level expression and efficient assembly of Hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. Bio/technology 5, 479-485.
7. Cregg, J.M. and Madden, K.N. (1988) Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins. Dev. Ind. Microbiol. 29, 33-41.
8. Cregg, J.M., Vedvick, T.S. and Raschke, W.C. (1993) Recent advances in the expression of foreign genes in Pichia pastoris. Bio/technology 11, 905-910.
9. Despreaux, C.W. and Manning, R.F. (1993) The dacA gene of Bacillus stearothermophilus coding for D-alanine carboxypeptidase: cloning, structure and expression in Escherichia coli and Pichia pastoris. Gene 131, 35-41.
10. Digan, M.E., Tscopp, J., Grinna, L., Lair, S.V., Craig, W.S., Velicelebi, G., Siegel, R.S., Davis, G.R. and Thill, G.P. (1988) Secretion of heterologous proteins from the methylotrophic yeast, Pichia pastoris. Dev. Ind. Microbiol. 29, 59-65.
11. Digan, M.E., Lair, S.V., Brierley, R.A., Siegel, R.S., Williams, M.E., Ellis, S.B., Kellaris, P.A., Provow, S.A., Craig, W.S., Velicelebi, G., Harpold, M.M. and Thill, G.P. (1989) Continous production of a novel lysozyme via secretion from the yeast Pichia pastoris. Bio/technology 7, 160-164.
12. Cregg, J.M. and Madden, K.N. (1987) Development of transformation systems and construction of methanol-utilisation-defective mutants of Pichia pastoris by gene disruption. In Stewart, G.G., Russel, I., Klein, R.D. and Hiebsch, R.R. (Eds) Biological Research on Industrial Yeast., Vol. II (CRC Press) pages1-18.
13. Hagenson, M.J., Holden, K.A., Parker, K.A., Wood, P.J., Cruze, J.A., Fuke, M., Hopkins, T.R. and Stroman, D.W. (1989) Expression of streptokinase in Pichia pastoris yeast. Enzyme Microbiol. Technol. 11, 650-656.
14. Hensing, M.C.M., Rouvenhorst, R.J., Heijnen, J.J., van Dijken, J.P. and Pronk, J.T. (1995) Physiological and technological aspects of large-scale heterologous-protein production with yeasts. Antonie van Leenwenhoek 67, 261-279.
15. Hitzeman, R.A., Hagie, F.F., Levine, H.L., Goeddel, D.W., Ammerer, G. and Hall, B.D. (1981) Expression of human gene for interferon in yeast. Nature 293, 717-723.
16. Hopp, T.P., Prickett, K.S., Price, V.L., Libby, R.T., March, C.J., Cerretti, D.P., Urdal, D.L. and Conlan, P.J. (1988) A short polypeptide marker sequence useful for recombinant protein identification and purification. Bio/technology 6, 1204-1210.
17. Janknecht, R., Martynoff, G., Lou, J., Hipskin, R.A. and Nordheim, A. (1991) Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. USA 88, 8972-8976.
18. Laroche, Y., Storme, V., Meutter, J., Messens, J. and Lauwereys, M. (1994) High-level secretion of and very efficient isotopic labelling of tick anticoagulant peptide (TAP) expressed in the methylotrophic yeast, Picha pastoris. Bio/technology 12, 1119-1124.
19. Kukuruzinska, M.A., Bergh, M.L.E. and Jackson, B.J. (1987) Protein glycosylation in yeast. Ann. Rev. Biochem. 56, 915-944.
20. Mardon, C.J. (1995) Automated protocols for the production of recombinant proteins from Pichia pastoris. Australasian Biotech. 5, 95-98.
21. Ogata, K., Nishikawa, H. and Ohsugi, M. (1969) A yeast capable of utilising methanol. Agric. Biol. Chem. 33, 1519-1520.
22. Ohsawa, I., Hirose, Y., Ishiguro, M., Imai, Y., Ishiura, S. and Kohsaka,S. (1995) Expression, purification and neuro trophic activity of amyloid precursor protein-secreted forms produced by yeast. Biochem. Biophys. Res. Commun. 213, 52-58.
23. Paifer, E., Margolles, E., Cremata, J., Montesino, R., Herrera, L. and Delgado, J.M. (1994) Efficient expression and secretion of recombinant alpha amylase in Pichia pastoris using two different signal sequences. Yeast 10, 1415-1419.
24. Ridder, R., Schmitz, R., Legay, F. and Gram, H. (1995) Generation of rabbit monoclonal antibody fragments from a combinatorial phage display and their production in the yeast Pichia pastoris. Bio/technology 13, 255-260.
25. Romanas, M.A., Clare, J.J., Beesley, K.M., Rayment, F.B., Ballantine, S.P., Makoff, A.J., Dougan, G., Fairweather, N.F. and Charles, I.G. (1991) Recombinant Bordetella pertussis pertactin (P69) from the yeast Pichia pastoris: high level production and immunological properties. Vaccine 9, 901-906.
26. Romanas, M.A., Scorer, C.A. and Clare, J.J. (1992) Foreign gene expression in yeast: a review. Yeast 8, 423-488.
27. Scorer, C.A., Buckholz, R.G., Clare, J.J. and Romanas, M. A. (1993) The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene 136, 111-119.
28. Siegel, R.S. and Brierley, R.A. (1989) Methylotrophic yeast Pichia pastoris produced in high-cell-density fermentations with high cell yields as vehicle for recombinant protein production. Biotech. Bioeng. 34, 403-404.
29. Sreekrishna, K., Nelles, L., Potenz, R., Cruze, J., Mazzaferro, P., Fish, W., Fuke, M.; Holden, K., Phelps, D., Wood, P. and Parker, K. (1989) High-level expression, purification and characterisation of recombinant human tumor necrosis factor synthesised in the methylotrophic yeast Pichia pastoris. Biochemistry 28, 4117-4125.
30. Sun, J., Coughlin, P., Salem, H.H. and Bird, P. (1995) Production and characterisation of recombinant human proteinase inhibitor 6 expressed in Pichia pastoris. Biochem. Biophys. Acta 1252, 28-34.
31. Talmadge, K., Stahl, S. and Gilbert, W. (1980) Eukaryotic signal sequence transports insulin antigen in Escherichia coli. Proc. Natl. Acad. Sci. USA 77, 3369-3373.
32. Thill, G.P., Davis, G.R., Stillman,. C., Holtz, G., Brierley, R., Engel, M., Buckholz, R.G., Kenney, J., Provow, S., Vedvick, T. and Siegel, R.S. (1990) Positive and negative effects of multi-copy integrated expression vectors of protein expression in Pichia pastoris. In Proceedings of the 6th International Symposium on Genetics of Microorganisms, Vol II. Heslot, H., Davies, J., Florent, J., Bobichon, L., Durand, G. Penasse, L. (Eds. Societe Francaise de Microbiologie, Paris) pages 477-490.
33. Tschopp, J.F., Brust, P.F., Cregg, J.M., Stillman, C. and Gingeras, T.R. (1987a) Expression of the LacZ gene from two methanol regulated promoters in Pichia pastoris. Nucleic Acid Res, 15, 3859-3876.
34. Tschopp,J.F., Sverlow,G., Kosson,R., Craig,W. and Grinna, L. (1987b) High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. Bio/technology 5, 1305-1308.
35. Schein, C.H., Kashiwagi, K., Fujisawa, A. and Weissmann, C. (1986) Secretion of mature IFN-a2 and accumulation of uncleaved precursor by Bacillus subtilis transformed with a hybrid a-amylase signal sequence-IFN-a2 gene. Bio/technology 4, 719-725.
36. Vedvick, T., Buckholz, R.G., Engel, M., Urcan, M., Kinney, J., Provow, S., Siegel, R.S. and Thill, G.P. (1991) High-level secretion of biologically active aprotinin from the yeast Pichia pastoris. J. Ind. Microbiol. 7, 197-201.
37. Vozza, L.A., Wittwer, L., Higgins, D.R., Purcell, T.J., Bergseid, M., Collinscracie, L.A., Lavallie, E.R. and Hoeffler, J.P. (1996) Production of a recombinant bovine enterokinase catalytic subunit in the methylotrophic yeast Pichia pastoris. Bio/technology 14, 77-81.
38. Wagner, S.L., Siegel, R.S., Vedvick, T.S., Raschke, W.C. and Van Nostrand, W.E. (1992) High-level expression, purification and characterisation of the Kunitz-type protease inhibitor domain of protease nexin-2/ amyloid b-protein precursor. Biochem. Biophys. Res. Commun. 186, 1138-1145.
39. Weiss, H.M., Haase, W., Michel, H. and Reilander, H. (1995) Expression of functional mouse 5-HT5 A serotonin receptor in the methylotrophic yeast Pichia pastoris: pharmacological characterisation and localisation. FEBS Letters 377, 451-456.
40. White, C.E., Kempi, N.M. and Komives, E. A. (1994) Expression of highly disulphide-bonded proteins in Pichia pastoris. Structure 2, 1003-1005.
41. Yamada, M., Azuma, T., Matsuba, T., Iida, H., Suzuki, H., Yamamoto, K., Kohli, Y. and Hori, H. (1994) Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast, Pichia pastoris and characterisation of produced recombinant cathepsin E. Biochem. Biophys. Acta 1206, 279-285.
42. Zhu, A., Monahan, C., Zhang, Z.F., Hurst, R., Leng, L. and Goldstein, J. (1995) High-level expression and purification of coffee bean alpha-galactosidase produced in the yeast Pichia pastoris. Archives Biochem. Biophys. 324, 65-70.