Ser11 plays several roles in the catalytic mechanism of rat glutathione transferase T2-2

Chemico-Biological Interactions

Volumn 133, Issue 1-3, 2001, Pages 178-181

  Jemth, Per ^a   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

Catalysis; Pre steady state kinetics; Product release; Theta class glutathione transferase

Indexed keywords

EID: 0003140521     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (4)

1. P. Jemth, G. Stenberg, G. Chaga, B. Mannervik, Heterologous expression, purification and characterization of rat class Theta glutathione transferase T2-2, Biochem. J. 316 (1996) 131-136.
2. P. Jemth, B. Mannervik, Fast product formation and slow product release are important features in a hysteretic reaction mechanism of GST T2-2, Biochemistry 38 (1999) 9982-9991.
3. P. Jemth, B. Mannervik, Active site serine promotes stabilization of the reactive glutathione thiolate in rat glutathione transferase T2-2. Evidence against proposed sulfatase activity of the corresponding human enzyme, J. Biol. Chem. 275 (2000) 8618-8624.
4. J.F. Parsons, G. Xiao, G.L. Gilliland, R.N. Armstrong, Enzymes harboring unnatural amino acids: mechanistic and structural analysis of the enhanced catalytic activity of a glutathione transferase containing 5-fluorotryptophan, Biochemistry 37 (1998) 6286-6294.