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note
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An anonymous referee suggests that the smaller amounts of ice which formed in the trehalose and sucrose solutions occur because the point at which ice first nucleates places the resulting solutions closer to the glass transition for the trehalose - water and sucrose - water systems than it does for the glucose - water and fructose - water systems. This would cause the disaccharide - water solutions which were in the process of freeze-concentrating to fall out of equilibrium with respect to the metastable ice - water liquidus at a higher temperature than the monosaccnaride - water solutions, and this would account for the significantly different amounts of frozen water in the different solutions. We thank the referee for this suggestion.
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